Abstract
This article describes the use of microfluidic paper-based analytical devices (μPADs) to perform quantitative chemical assays with internal standards. MicroPADs are well-suited for colorimetric biochemical assays; however, errors can be introduced from the background color of the paper due to batch difference and age, and from color measurement devices. To reduce errors from these sources, a series of standard analyte solutions and the sample solution are assayed on a single device with multiple detection zones simultaneously; an analyte concentration calibration curve can thus be established from the standards. Since the μPAD design allows the colorimetric measurements of the standards and the sample to be conducted simultaneously and under the same condition, errors from the above sources can be minimized. The analytical approach reported in this work shows that μPADs can perform quantitative chemical analysis at very low cost.
Similar content being viewed by others
References
Martinez AW, Phillips ST, Butte M, Whitesides GM (2007) Angew Chem Int Ed 46:1318–1320
Martinez AW, Phillips ST, Carrilho E, Thomas SW III, Sindi H, Whitesides GM (2008) Anal Chem 80:3699–3707
Li X, Tian J, Nguyen T, Shen W (2008) Anal Chem 80:9131–9134
Shen W, Tian J, Li X, Khan MS, Garnier G (2009) Method of fabricating microfluidic systems PCT/AU2009/000889
Dittrich PS (2009) Lab Chip 9:15–16
Abe K, Suzuki K, Citterio D (2008) Anal Chem 80:6928–6934
Blicharz TM, Rissin DM, Bowden M, Hayman RB, DiCesare C, Bhatia JS, Grand-Pierre N, Siqueira WL, Helmerhorst EJ, Loscalzo J, Oppenheim FG, Walt DR (2008) Clin Chem 54:1473–1480
Nagler RM (2008) Clin Chem 54:1415–1417
Khan MS, Fon D, Li X, Forsythe J, Thouas G, Garnier G, Shen W (2008) Chemeca 2008: Towards a Sustainable Australasia 744–753
Shen W, Filonanko Y, Truong Y, Parker IH (2000) Colloid and Surface A: Phys Eng Asp 173:117–126
Kannangara D, Zhang H, Shen W (2006) Colloid and Surface A: Phys Eng Asp 280:203–215
Sun J, Zhang X, Broderick M, Fein H (2003) Sensors 3:276–284
Gindler EM (1970) Clin Chem 16:536
Acknowledgments
The research scholarships of Monash University and the Department of Chemical Engineering are gratefully acknowledged. BASF and Hercules Australia are thanked for providing the hydrophobization agents.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li, X., Tian, J. & Shen, W. Quantitative biomarker assay with microfluidic paper-based analytical devices. Anal Bioanal Chem 396, 495–501 (2010). https://doi.org/10.1007/s00216-009-3195-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-009-3195-9