Skip to main content

Advertisement

SpringerLink
Log in

RETRACTED ARTICLE: Ameliorative effect of two structurally divergent hydrazide derivatives against DSS-induced colitis by targeting Nrf2 and NF-κB signaling in mice

  • Original Article
  • Published:
Naunyn-Schmiedeberg's Archives of Pharmacology Aims and scope Submit manuscript

This article was retracted on 10 January 2024

This article has been updated

Abstract

The environmental factors and genetic vulnerability trigger the inflammatory bowel diseases (IBDs) such as ulcerative colitis and Crohn’s disease. Furthermore, the oxidative stress and inflammatory cytokines have been implicated in the aggravation of the IBDs. The aim of the present study was to investigate the effect of N-(benzylidene)-2-((2-hydroxynaphthalen-1-yl)diazenyl)benzohydrazides (NCHDH and NTHDH) compounds against the DSS-induced colitis in mice. The colitis was induced by 5% dextran sulfate sodium (DSS) dissolved in normal saline for 5 days. The effect of the NCHDH and NTHDH on the behavioral, biochemical, histological, and immunohistological parameters was assessed. The NCHDH and NTHDH treatment improved the behavioral parameters such as food intake, disease activity index, and diarrhea score significantly compared to DSS control. The NCHDH and NTHDH treatments significantly increased the antioxidant enzymes, whereas oxidative stress markers were markedly reduced. Similarly, the NCHDH and NTHDH treatments significantly suppressed the activity of nitric oxide (NO), myeloperoxidase (MPO), and eosinophil peroxidase (EPO). The histological studies showed a significant reduction in inflammation, immune cell infiltration, and fibrosis in the NCHDH- and NTHDH-treated groups. The immunohistochemical results demonstrated that NCHDH and NTHDH treatments markedly increase the expression level of Nrf2, HO-1 (hemeoxygenase-1), TRX (thioredoxin reductase), and IκB compared to the DSS-induced group. In the same way, the NCHDH and NTHDH significantly reduced the NF-κB and COX-2 (cyclooxygenase-2) expression levels. The NCHDH and NTHDH treatment significantly improved the symptoms associated with colitis via inducing antioxidants and attenuating oxidative stress markers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12
Fig. 13
Fig. 14

Similar content being viewed by others

Data availability

Not applicable.

Change history

Abbreviations

IBD:

Inflammatory bowel diseases

Nrf2:

Nuclear factor erythroid 2-related factor 2

TNF-α:

Tumor necrosis factor-α

COX-2:

Cyclooxygenase-2

NF-κB:

Nuclear factor kappa-light-chain-enhancer of activated B cells

GST:

Glutathione-S-transferases

ARE:

Antioxidant response element

CTAB:

Cetyltrimethylammonium bromide

MPO:

Myeloperoxidase

NO:

Nitric oxide

SOD:

Sulfur oxide dismutase

POD:

Peroxidase

LPO:

Lipid peroxidase

EPO:

Eosinophil peroxidase

HO-1:

Hemeoxygenase-1

PAS:

Periodic acid-Schiff

MDA:

Malondialdehyde

DSS:

Dextran sodium sulfate

TRX:

Thioredoxin reductase

References

  • Afridi R, Khan AU, Khalid S, Shal B, Rasheed H, Ullah MZ, Shehzad O, Kim YS, Khan S (2019) Anti-hyperalgesic properties of a flavanone derivative poncirin in acute and chronic inflammatory pain models in mice. BMC Pharmacol Toxicol 20:57

  • Ali J, Khan AU, Shah FA, Ali H, Islam SU, Kim YS, Khan S (2019) Mucoprotective effects of saikosaponin-A in 5-fluorouracil-induced intestinal mucositis in mice model. Life Sci 239:116888

  • Ali H, Khan A, Ali J, Ullah H, Khan A, Ali H, Irshad N, Khan S (2020) Attenuation of LPS-induced acute lung injury by continentalic acid in rodents through inhibition of inflammatory mediators correlates with increased Nrf2 protein expression. BMC Pharmacol Toxicol 21:1–14

    Article  Google Scholar 

  • Andújar I, Ríos JL, Giner RM, Miguel Cerda J, Recio MdC (2012) Beneficial effect of shikonin on experimental colitis induced by dextran sulfate sodium in BALB/c mice. Evid Based Complement Alternat Med 2012:1–15

    Article  Google Scholar 

  • Arafa HM, Hemeida RA, El-Bahrawy AI, Hamada FM (2009) Prophylactic role of curcumin in dextran sulfate sodium (DSS)-induced ulcerative colitis murine model. Food Chem Toxicol 47:1311–1317

    Article  CAS  PubMed  Google Scholar 

  • Araldi GL, Liao Y, Brugger N (2008) Hydrazide derivatives as prostaglandin receptors modulators. Google Patents (WO 2005/012232)

  • Atiq A, Shal B, Naveed M, Khan A, Ali J, Zeeshan S, Al-Sharari SD, Kim YS, Khan S (2019) Diadzein ameliorates 5-fluorouracil-induced intestinal mucositis by suppressing oxidative stress and inflammatory mediators in rodents. Eur J Pharmacol 843:292–306

    Article  CAS  PubMed  Google Scholar 

  • Barrett CW, Ning W, Chen X, Smith JJ, Washington MK, Hill KE, Coburn LA, Peek RM, Chaturvedi R, Wilson KT (2013) Tumor suppressor function of the plasma glutathione peroxidase gpx3 in colitis-associated carcinoma. Cancer Res 73:1245–1255

    Article  CAS  PubMed  Google Scholar 

  • Bento AF, Leite DFP, Claudino RF, Hara DB, Leal PC, Calixto JB (2008) The selective nonpeptide CXCR2 antagonist SB225002 ameliorates acute experimental colitis in mice. J Leukoc Biol 84:1213–1221

    Article  CAS  PubMed  Google Scholar 

  • Camuesco D, Gálvez J, Nieto A, Mn C, Rodríguez-Cabezas ME, Concha A, Xaus J, Zarzuelo A (2005) Dietary olive oil supplemented with fish oil, rich in EPA and DHA (n-3) polyunsaturated fatty acids, attenuates colonic inflammation in rats with DSS-induced colitis. J Nutr 135:687–694

    Article  CAS  PubMed  Google Scholar 

  • Chang C-J, Wang P-C, Huang T-C, Taniguchi A (2019) Change in renal glomerular collagens and glomerular filtration barrier-related proteins in a dextran sulfate sodium-induced colitis mouse model. Int J Mol Sci 20:1458

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Chassaing B, Aitken JD, Malleshappa M, Vijay-Kumar M (2014) Dextran sulfate sodium (DSS)-induced colitis in mice. Curr Protoc Immunol 104:15.25. 11-15.25. 14

    Google Scholar 

  • Cheemanapalli S, Chinthakunta N, Shaikh NM, Shivaranjani V, Pamuru RR, Chitta SK (2019) Comparative binding studies of curcumin and tangeretin on up-stream elements of NF-kB cascade: a combined molecular docking approach. Netw Model Anal Health Inform Bioinformatics 8:15

    Article  Google Scholar 

  • Creyns B, Cremer J, Hoshino T, Geboes K, de Hertogh G, Ferrante M, Vermeire S, Ceuppens JL, Van Assche G, Breynaert C (2019) Fibrogenesis in chronic DSS colitis is not influenced by neutralisation of regulatory T cells, of major T helper cytokines or absence of IL-13. Sci Rep 9:1–14

    Article  CAS  Google Scholar 

  • de Bruyn M, Breynaert C, Arijs I, De Hertogh G, Geboes K, Thijs G, Matteoli G, Hu J, Van Damme J, Arnold B (2017) Inhibition of gelatinase B/MMP-9 does not attenuate colitis in murine models of inflammatory bowel disease. Nat Commun 8:15384

    Article  PubMed  PubMed Central  Google Scholar 

  • Deguchi Y, Andoh A, Inatomi O, Yagi Y, Bamba S, Araki Y, Hata K, Tsujikawa T, Fujiyama Y (2007) Curcumin prevents the development of dextran sulfate sodium (DSS)-induced experimental colitis. Dig Dis Sci 52:2993–2998

    Article  CAS  PubMed  Google Scholar 

  • Elian S, Souza E, Vieira A, Teixeira M, Arantes R, Nicoli J, Martins F (2014) Bifidobacterium longum subsp. infantis BB-02 attenuates acute murine experimental model of inflammatory bowel disease. Benefic Microbes 6:277–286

    Article  Google Scholar 

  • Guo L, Ye C, Hao X, Zheng R, Ju R, Wu D, Luo L, Wang C, Li J, Yu X (2012) Carboxyamidotriazole ameliorates experimental colitis by inhibition of cytokine production, nuclear factor-κB activation, and colonic fibrosis. J Pharmacol Exp Ther 342:356–365

    Article  CAS  PubMed  Google Scholar 

  • Hirata I, Murano M, Nitta M, Sasaki S-i, Toshina K, Maemura K, Katsu K-i (2001) Estimation of mucosal inflammatory mediators in rat DSS-induced colitis. Digestion 63:73–80

    Article  CAS  PubMed  Google Scholar 

  • Ijiri K, Potten C (1987) Further studies on the response of intestinal crypt cells of different hierarchical status to eighteen different cytotoxic agents. Br J Cancer 55:113–123

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ito R, Kita M, Shin-Ya M, Kishida T, Urano A, Takada R, Sakagami J, Imanishi J, Iwakura Y, Okanoue T (2008) Involvement of IL-17A in the pathogenesis of DSS-induced colitis in mice. Biochem Biophys Res Commun 377:12–16

    Article  CAS  PubMed  Google Scholar 

  • Jawhara S, Thuru X, Standaert-Vitse A, Jouault T, Mordon S, Sendid B, Desreumaux P, Poulain D (2008) Colonization of mice by Candida albicans is promoted by chemically induced colitis and augments inflammatory responses through galectin-3. J Infect Dis 197:972–980

    Article  CAS  PubMed  Google Scholar 

  • Joo Y-E, Karrasch T, Mühlbauer M, Allard B, Narula A, Herfarth HH, Jobin C (2009) Tomato lycopene extract prevents lipopolysaccharide-induced NF-κB signaling but worsens dextran sulfate sodium-induced colitis in NF-κBEGFP mice. PLoS One 4(2):e4562. https://doi.org/10.1371/journal.pone.0004562

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ju S, Mu J, Dokland T, Zhuang X, Wang Q, Jiang H, Xiang X, Deng Z-B, Wang B, Zhang L (2013) Grape exosome-like nanoparticles induce intestinal stem cells and protect mice from DSS-induced colitis. Mol Ther 21:1345–1357

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Khan A, Khan A, Shal B, Aziz A, Ahmed MN, Khan S (2022a) N‐(benzylidene)‐2‐((2‐hydroxynaphthalen‐1‐yl) diazenyl) benzohydrazides (1‐2)(NCHDH and NTHDH) attenuate DMBA‐induced breast cancer via Nrf2/NF‐κB/apoptosis signaling

  • Khan ZU, Razzaq A, Khan A, Rehman NU, Khan H, Khan T, Khan AU, Althobaiti NA, Menaa F, Iqbal H (2022b) Physicochemical characterizations and pharmacokinetic evaluation of pentazocine solid lipid nanoparticles against inflammatory pain model. Pharmaceutics 14:409

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Khan A, Ullah MZ, Afridi R, Rasheed H, Khalid S, Ullah H, Ali H, AlSharari SD, Kim YS, Khan S (2019) Antinociceptive properties of 25-methoxy hispidol A, a triterpinoid isolated from Poncirus trifoliata (Rutaceae) through inhibition of NF-κB signalling in mice. Phytother Res 33:327–341

    Article  CAS  PubMed  Google Scholar 

  • Khan AM, Khan AU, Ali H, Islam SU, Seo EK, Khan S (2020a) Continentalic acid exhibited nephroprotective activity against the LPS and E. coli-induced kidney injury through inhibition of the oxidative stress and inflammation. Int Immunopharmacol 80:106209

    Article  CAS  PubMed  Google Scholar 

  • Khan AU, Muhammad Khan A, Khan A, Shal B, Aziz A, Ahmed MN, Khan S (2020b) The newly synthesized compounds (NCHDH and NTHDH) attenuates LPS-induced septicemia and multi-organ failure via Nrf2/HO1 and HSP/TRVP1 signaling in mice. Chem Biol Interact 329:109220

    Article  CAS  PubMed  Google Scholar 

  • Khan AU, Khan A, Khan A, Shal B, Aziz A, Ahmed MN, Islam SU, Ali H, Shehzad A, Khan S (2021) Inhibition of NF-κB signaling and HSP70/HSP90 proteins by newly synthesized hydrazide derivatives in arthritis model. 1–23

  • Khan S, Shehzad O, Chun J, Kim YS (2013) Mechanism underlying anti-hyperalgesic and anti-allodynic properties of anomalin in both acute and chronic inflammatory pain models in mice through inhibition of NF-κB, MAPKs and CREB signaling cascades. Eur J Pharmacol 718:448–458

    Article  CAS  PubMed  Google Scholar 

  • Khan S, Shin EM, Choi RJ, Jung YH, Kim J, Tosun A, Kim YS (2011) Suppression of LPS-induced inflammatory and NF-κB responses by anomalin in RAW 264.7 macrophages. J Cell Biochem 112:2179–2188

    Article  CAS  PubMed  Google Scholar 

  • Khor TO, Huang M-T, Kwon KH, Chan JY, Reddy BS, Kong A-N (2006) Nrf2-deficient mice have an increased susceptibility to dextran sulfate sodium–induced colitis. Can Res 66:11580–11584

    Article  CAS  Google Scholar 

  • Kim CJ, Kovacs-Nolan JA, Yang C, Archbold T, Fan MZ, Mine Y (2010) l-Tryptophan exhibits therapeutic function in a porcine model of dextran sodium sulfate (DSS)-induced colitis. J Nutr Biochem 21:468–475

    Article  CAS  PubMed  Google Scholar 

  • Kimura T, Ishii Y, Morishima Y, Shibuya A, Shibuya K, Taniguchi M, Mochizuki M, Hegab AE, Sakamoto T, Nomura A (2004) Treatment with α-galactosylceramide attenuates the development of bleomycin-induced pulmonary fibrosis. J Immunol 172:5782–5789

    Article  CAS  PubMed  Google Scholar 

  • Kumar A, Elangaimannan R (2017) A review on biological activities of hydrazone derivatives. Int J Pharm Clin Res 9:183–192

    Google Scholar 

  • Lan A, Blais A, Coelho D, Capron J, Maarouf M, Benamouzig R, Lancha AH Jr, Walker F, Tomé D, Blachier F (2016) Dual effects of a high-protein diet on DSS-treated mice during colitis resolution phase. Am J Physiol Gastrointest Liver Physiol 311:G624–G633

    Article  PubMed  Google Scholar 

  • Larrosa M, Yañéz-Gascón MJ, Selma MV, González-Sarrías A, Toti S, Cerón JJ, Tomás-Barberán F, Dolara P, Espín JC (2009) Effect of a low dose of dietary resveratrol on colon microbiota, inflammation and tissue damage in a DSS-induced colitis rat model. J Agric Food Chem 57(6):2211–2220. https://doi.org/10.1021/jf803638d

    Article  CAS  PubMed  Google Scholar 

  • Lawrance IC, West G, Willis J, Fiocchi C, Chakravarti S (2000) A novel murine model of chronic inflammation-induced intestinal fibrosis. Gastroenterology 118:A575

    Article  Google Scholar 

  • Li B, Alli R, Vogel P, Geiger TL (2014) IL-10 modulates DSS-induced colitis through a macrophage–ROS–NO axis. Mucosal Immunol 7:869

    Article  CAS  PubMed  Google Scholar 

  • Liu X-c, Mei Q, Xu J-m, Hu J (2009) Balsalazine decreases intestinal mucosal permeability of dextran sulfate sodium-induced colitis in mice. Acta Pharmacol Sin 30:987

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Londhe AM, Gadhe CG, Lim SM, Pae AN (2019) Investigation of molecular details of Keap1-Nrf2 inhibitors using molecular dynamics and umbrella sampling techniques. Molecules 24:4085

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Murano M, Maemura K, Hirata I, Toshina K, Nishikawa T, Hamamoto N, Sasaki S, Saitoh O, Katsu K (2000) Therapeutic effect of intracolonically administered nuclear factor κB (p65) antisense oligonucleotide on mouse dextran sulphate sodium (DSS)-induced colitis. Clin Exp Immunol 120:51–58

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nerandzic MM, Donskey CJ (2009) Effective and reduced-cost modified selective medium for isolation of Clostridium difficile. J Clin Microbiol 47:397–400

    Article  CAS  PubMed  Google Scholar 

  • Ogawa Y, Kanatsu K, Iino T, Kato S, Jeong Y, Shibata N, Takada K, Takeuchi K (2002) Protection against dextran sulfate sodium-induced colitis by microspheres of ellagic acid in rats. Life Sci 71:827–839

    Article  CAS  PubMed  Google Scholar 

  • Osman N, Adawi D, Ahrné S, Jeppsson B, Molin G (2008) Probiotics and blueberry attenuate the severity of dextran sulfate sodium (DSS)-induced colitis. Dig Dis Sci 53:2464–2473

    Article  PubMed  Google Scholar 

  • Osman N, Adawi D, Molin G, Ahrne S, Berggren A, Jeppsson B (2006) Bifidobacterium infantis strains with and without a combination of oligofructose and inulin (OFI) attenuate inflammation in DSS-induced colitis in rats. BMC Gastroenterol 6:31

    Article  PubMed  PubMed Central  Google Scholar 

  • Palić D, Andreasen CB, Ostojić J, Tell RM, Roth JA (2007) Zebrafish (Danio rerio) whole kidney assays to measure neutrophil extracellular trap release and degranulation of primary granules. J Immunol Methods 319:87–97

    Article  PubMed  Google Scholar 

  • Rasheed H, Afridi R, Khan AU, Ullah MZ, Khalid S, Atiq A, Kashif H, Ahmed MN, Kim YS, Khan S (2018) Anti-inflammatory, anti-rheumatic and analgesic activities of 2-(5-mercapto-1, 3, 4-oxadiazol-2-yl)-N-propylbenzenesulphonamide (MOPBS) in rodents. Inflammopharmacology 26:1037–1049

    Article  CAS  PubMed  Google Scholar 

  • Rees V (1998) Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines. Clin Exp Immunol 114:385–391

    PubMed  Google Scholar 

  • Renes IB, Boshuizen JA, Van Nispen DJ, Bulsing NP, Buller HA, Dekker J, Einerhand AW (2002) Alterations in Muc2 biosynthesis and secretion during dextran sulfate sodium-induced colitis. Am J Physiol Gastrointest Liver Physiol 282:G382–G389

    Article  CAS  PubMed  Google Scholar 

  • Rochat T, Bermúdez-Humarán L, Gratadoux J-J, Fourage C, Hoebler C, Corthier G, Langella P (2007) Anti-inflammatory effects of Lactobacillus casei BL23 producing or not a manganese-dependant catalase on DSS-induced colitis in mice. Microb Cell Fact 6:22

    Article  PubMed  PubMed Central  Google Scholar 

  • Rollas S, Küçükgüzel SG (2007) Biological activities of hydrazone derivatives. Molecules 12:1910–1939

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rumi G, Tsubouchi R, Okayama M, Kato S, Mózsik G, Takeuchi K (2004) Protective effect of lactulose on dextran sulfate sodium-induced colonic inflammation in rats. Dig Dis Sci 49:1466–1472

    Article  CAS  PubMed  Google Scholar 

  • Saffari H, Leiferman KM, Clayton F, Baer K, Leonard F III, Gleich GJ, Peterson KA (2016) Measurement of inflammation in eosinophilic esophagitis using an eosinophil peroxidase assay. Am J Gastroenterol 111:933–939

    Article  CAS  PubMed  Google Scholar 

  • Sana E, Zeeshan M, Ain QU, Khan AU, Hussain I, Khan S, Lepeltier E, Ali H (2021) Topical delivery of curcumin-loaded transfersomes gel ameliorated rheumatoid arthritis by inhibiting NF-kappabeta pathway. Nanomedicine (Lond) 16:819–837

    Article  CAS  PubMed  Google Scholar 

  • Sánchez-Fidalgo S, Cárdeno A, Sánchez-Hidalgo M, Aparicio-Soto M, de la Lastra CA (2013) Dietary extra virgin olive oil polyphenols supplementation modulates DSS-induced chronic colitis in mice. J Nutr Biochem 24:1401–1413

    Article  PubMed  Google Scholar 

  • Schreiber O, Petersson J, Waldén T, Ahl D, Sandler S, Phillipson M, Holm L (2013) iNOS-dependent increase in colonic mucus thickness in DSS-colitic rats. PLoS ONE 8:e71843

    Article  PubMed  PubMed Central  Google Scholar 

  • Sedhom MA, Pichery M, Murdoch JR, Foligné B, Ortega N, Normand S, Mertz K, Sanmugalingam D, Brault L, Grandjean T (2013) Neutralisation of the interleukin-33/ST2 pathway ameliorates experimental colitis through enhancement of mucosal healing in mice. Gut 62:1714–1723

    Article  CAS  PubMed  Google Scholar 

  • Seril DN, Liao J, Ho K-LK, Warsi A, Yang CS, Yang G-Y (2002) Dietary iron supplementation enhances DSS-induced colitis and associated colorectal carcinoma development in mice. Dig Dis Sci 47:1266–1278

    Article  CAS  PubMed  Google Scholar 

  • Shal B, Khan A, Khan AU, Ullah R, Ali G, Islam SU, Haq Iu, Ali H, Seo E-K, Khan S (2021) Alleviation of memory deficit by bergenin via the regulation of reelin and Nrf-2/NF-κB pathway in transgenic mouse model. Int J Mol Sci 22:6603

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shea-Donohue T, Thomas K, Cody MJ, Zhao A, DeTolla LJ, Kopydlowski KM, Fukata M, Lira SA, Vogel SN (2008) Mice deficient in the CXCR2 ligand, CXCL1 (KC/GRO-α), exhibit increased susceptibility to dextran sodium sulfate (DSS)-induced colitis. Innate Immun 14:117–124

    Article  CAS  PubMed  Google Scholar 

  • Singh N, Ranjana R, Kumari M, Kumar B (2016) A review on biological activities of hydrazone derivatives. Int J Pharm Clin Res 8:162–166

    Google Scholar 

  • Soriano A, Salas A, Salas A, Sans M, Gironella M, Elena M, Anderson DC, Piqué JM, Panés J (2000) VCAM-1, but not ICAM-1 or MAdCAM-1, immunoblockade ameliorates DSS-induced colitis in mice. Lab Invest 80:1541

    Article  CAS  PubMed  Google Scholar 

  • Stevceva L, Pavli P, Husband AJ, Doe WF (2001) The inflammatory infiltrate in the acute stage of the dextran sulphate sodium induced colitis: B cell response differs depending on the percentage of DSS used to induce it. BMC Clin Pathol 1:3

    Article  PubMed  PubMed Central  Google Scholar 

  • Tonolo F, Folda A, Cesaro L, Scalcon V, Marin O, Ferro S, Bindoli A, Rigobello MP (2020) Milk-derived bioactive peptides exhibit antioxidant activity through the Keap1-Nrf2 signaling pathway. J Funct Foods 64:103696

    Article  CAS  Google Scholar 

  • Ullah H, Khan A, Baig MW, Ullah N, Ahmed N, Tipu MK, Ali H, Khan S (2020) Poncirin attenuates CCL4-induced liver injury through inhibition of oxidative stress and inflammatory cytokines in mice. BMC Complement Med Ther 20:1–14

    Article  Google Scholar 

  • Ullah MZ, Khan AU, Afridi R, Rasheed H, Khalid S, Naveed M, Ali H, Kim YS, Khan S (2018) Attenuation of inflammatory pain by puerarin in animal model of inflammation through inhibition of pro-inflammatory mediators. Int Immunopharmacol 61:306–316

    Article  CAS  PubMed  Google Scholar 

  • Varga SM, Beckman NA, Chu M, Braciale TJ (2002) Sensitive detection and quantitation of mouse eosinophils in tissues using an enzymatic eosinophil peroxidase assay: its use to rapidly measure pulmonary eosinophilia during experimental respiratory syncytial virus infection of mice. J Immunol Methods 262:111–120

    Article  CAS  PubMed  Google Scholar 

  • Verdu E, Bercik P, Cukrowska B, Farre-Castany M, Bouzourene H, Saraga E, Blum A, Corthésy-Theulaz I, Tlaskalova-Hogenova H, Michetti P (2000) Oral administration of antigens from intestinal flora anaerobic bacteria reduces the severity of experimental acute colitis in BALB/c mice. Clin Exp Immunol 120:46–50

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wei G, An P, Vaid KA, Nasser I, Huang P, Tan L, Zhao S, Schuppan D, Popov YV (2020) Comparison of murine steatohepatitis models identifies a dietary intervention with robust fibrosis, ductular reaction, and rapid progression to cirrhosis and cancer. Am J Physiol Gastrointest Liver Physiol 318:G174–G188

    Article  PubMed  Google Scholar 

  • Winkel AF, Engel CK, Margerie D, Kannt A, Szillat H, Glombik H, Kallus C, Ruf S, Güssregen S, Riedel J (2015) Characterization of RA839, a noncovalent small molecule binder to Keap1 and selective activator of Nrf2 signaling. J Biol Chem 290:28446–28455

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhang Y, Tao M, Chen C, Zhao X, Feng Q, Chen G, Fu Y (2022) BAFF blockade attenuates DSS-induced chronic colitis via inhibiting NLRP3 inflammasome and NF-κB activation. 672

  • Zhu S, Huang M, Feng G, Miao Y, Wu H, Zeng M, Lo YM (2018) Gelatin versus its two major degradation products, prolyl-hydroxyproline and glycine, as supportive therapy in experimental colitis in mice. Food Sci Nutr 6:1023–1031

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zimmermann M (1986) Ethical considerations in relation to pain in animal experimentation. Acta Physiol Scand Suppl 554:221–233

    CAS  PubMed  Google Scholar 

Download references

Funding

This work was assisted by Higher Education Commission (HEC), Pakistan, through HEC indigenous scholarship with funding (No. 518–85883-2MD5-039 (50043702)).

Author information

Authors and Affiliations

  1. Pharmacological Sciences Research Laboratory, Department of Pharmacy, Faculty of Biological Sciences, Quaid-I-Azam University, Islamabad, 45320, Pakistan

    Ashrafullah Khan, Adnan Khan, Bushra Shal & Salman Khan

  2. Faculty of Pharmaceutical Sciences, Abasyn University, Peshawar, 25000, KPK, Pakistan

    Ashrafullah Khan & Muhammad Usman Amin

  3. Department of Chemistry, The University of Azad Jammu and Kashmir, Muzaffarabad, 13100, Pakistan

    Abdul Aziz & Muhammad Naeem Ahmed

  4. Department of Health and Biological Sciences, Abasyn University, Peshawar, 25000, Pakistan

    Sajjad Ahmad

  5. Department of Chemistry, Quaid-I-Azam University, Islamabad, Pakistan

    Zia-ur-Rehman

Authors
  1. Ashrafullah Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Adnan Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bushra Shal
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Abdul Aziz
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sajjad Ahmad
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Muhammad Usman Amin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Muhammad Naeem Ahmed
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Zia-ur-Rehman
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Salman Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

AUK, AK, and BS performed the animal’s activities and biochemical analysis. SA and AUK performed the computational analysis. AZ, MNA, and ZUR synthesized and characterized the compounds. AUK and MUA revised the manuscript. Salman Khan drafted the manuscript and supervised the project. All authors read and approved the manuscript. All the data were generated in-house and that no paper mill was used.

Corresponding author

Correspondence to Salman Khan.

Ethics declarations

Ethics approval

All the experimental protocols were approved by the Quaid-i-Azam University, Islamabad (Pakistan) bioethical committee of animal care (BEC-FBS-QAU2019-193).

Consent to participate

Not applicable.

Consent for publications

Not applicable.

Competing interests

The authors declare no competing interests.

Additional information

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

This article has been retracted. Please see the retraction notice for more detail:https://doi.org/10.1007/s00210-024-02942-x

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (XLSX 56 KB)

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Khan, A., Khan, A., Shal, B. et al. RETRACTED ARTICLE: Ameliorative effect of two structurally divergent hydrazide derivatives against DSS-induced colitis by targeting Nrf2 and NF-κB signaling in mice. Naunyn-Schmiedeberg's Arch Pharmacol 395, 1167–1188 (2022). https://doi.org/10.1007/s00210-022-02272-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00210-022-02272-w

Keywords

Search

Navigation