Abstract
A Gram-stain-negative, catalase- and oxidase-positive, rod-shaped bacterium, designated as JK6T was isolated from a coastal marine sediment in Jeju Island. Strain JK6T was characterized by polyphasic investigation including genome features. It grew at pH 5.0–9.0 (optimum 7.5), 18–30 °C (optimum 25 °C) and 1.0–7.0% (w/v) NaCl (optimum 2.0%). Strain JK6T utilized d-mannose, d-glucose, l-fucose, propionate and acetate as carbon and energy sources. The sole quinone was ubiquinone-10, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, and sphingolipid. Strain JK6T was closely related to Parasphingorhabdus flavimaris SW-151T (98.2%), Parasphingorhabdus marina DSM 22363T (97.6%) and Parasphingorhabdus litoris FR1093T (97.6%) based on 16S rRNA gene sequence similarity. Genome length and GC content were 3.29 Mbp and 53.0%, respectively. Digital DNA-DNA relatedness, average nucleotide identity, and average amino acid identity between strain JK6T and P. flavimaris SW-151T were 16.6%, 73.9%, and 77.6%, respectively. These results showed that the strain can be recognized as a novel bacterium named Parasphingorhabdus halotolerans. The type strain of Parasphingorhabdus halotolerans sp. nov. is JK6T (= KCTC 72818T = VTCC 910111T).
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence and the complete genome of strain JK6T is MK905445 and CP051217.
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Funding
This work was supported by Grants from the National Research Foundation of Korea (No. 2020R1I1A3062110 to SJP) and the National Institute of Biological Resources funded by the Ministry of Environment (No. NIBR202102109 and NIBR202104104 to the SJP). This work was supported by the Basic Research Project of the Korea Institute of Geoscience and Mineral Resources (21–3412-1 to SJK).
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S-JK, MK and S-JP designed research; MK and S-JP performed research; S-JK, MK, K-EL and I-TC analyzed data; S-JK, MK and S-JP wrote the paper.
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Communicated by Erko Stackebrandt.
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Kim, SJ., Kim, M., Lee, KE. et al. Parasphingorhabdus halotolerans sp. nov. isolated from marine sediment in Jeju Island. Arch Microbiol 203, 3803–3809 (2021). https://doi.org/10.1007/s00203-021-02366-8
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DOI: https://doi.org/10.1007/s00203-021-02366-8