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Phyllobacterium pellucidum sp. nov., isolated from soil

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Abstract

A bacterial strain, BT25T, was isolated from soil in Korea. The bacterial cells were Gram-negative and rod-shaped. Phylogenetic analysis using 16S rRNA gene sequences showed that the BT25T strain was related to the genus Phyllobacterium. BT25T was 96.6 and 96.5% similar to Phyllobacterium brassicacearum STM 196T and Phyllobacterium myrsinacearum DSM 5892T, respectively. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between BT25T and the two closest phylogenetic neighbors were calculated to be 78.5 and 77.7, 21.1 and 21.2%, respectively. The major cellular fatty acids were summed feature 8 (C18:1 ω7c/C18:1 ω6c) (29.3%), cyclo-C19:0 ω8c (27.5%), and C16:0 (16.5%). The BT25T strain had menaquinone Q-10 as the predominant quinone, as well as phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, and phosphatidylcholine as the major polar lipids. Based on the phenotypic, phylogenetic, and chemotaxonomic data, the BT25T strain was classified as a novel Phyllobacterium species. The name Phyllobacterium pellucidum sp. nov. was proposed. The type strain is BT25T (= KCTC 62765T = NBRC 114381T).

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Acknowledgements

This work was supported by a research grant from Seoul Women’s University (2021) and by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR201902203).

We are grateful to Dr. Aharon Oren (The Hebrew University of Jerusalem, Israel) for helping with the etymology.

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Author notes

  1. Yuna Park and Leonid N. Ten are Co-authors who shared work equally.

Authors and Affiliations

  1. Department of Bio and Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul, 01797, Republic of Korea

    Yuna Park, Soohyun Maeng & Myung Kyum Kim

  2. School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea

    Leonid N. Ten & Hee‑Young Jung

  3. Department of Food and Nutrition, Kookmin University, Seoul, 02707, Republic of Korea

    Yoonjee Chang

  4. Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea

    Hee‑Young Jung

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  1. Yuna Park
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Correspondence to Yoonjee Chang, Hee‑Young Jung or Myung Kyum Kim.

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Communicated by Erko Stackebrandt.

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Park, Y., Ten, L.N., Maeng, S. et al. Phyllobacterium pellucidum sp. nov., isolated from soil. Arch Microbiol 203, 2647–2652 (2021). https://doi.org/10.1007/s00203-021-02205-w

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  • DOI: https://doi.org/10.1007/s00203-021-02205-w

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