Abstract
Key message
A novel wax locus GLOSSY1 was finely mapped to an approximately 308.1-kbp genomic interval on chromosome 2DS of wheat.
Abstract
The epicuticular wax, the outermost layer of aerial organs, gives plants their bluish-white (glaucous) appearance. Epicuticular wax is ubiquitous and provides an essential protective function against environmental stresses. In this study, we identified the glossy1 mutant on the basis of its glossy glume from an EMS population in the elite wheat (Triticum aestivum L.) cultivar Jimai22. The mutant had a dramatically different profile in total wax load and composition of individual wax constituents relative to the wild type, resulting in the increased cuticle permeability of glumes. The glossy glume phenotype was controlled by a single, semidominant locus mapping to the short arm of chromosome 2D, within a 308.1-kbp genomic interval that contained ten annotated protein-coding genes. These results pave the way for an in-depth analysis of the underlying genetic basis of wax formation patterns and enrich our understanding of mechanisms regulating wax metabolism.
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Acknowledgements
The authors would like to thank Prof. Jianxin Shi (Shanghai Jiao Tong University) for the GC-FID and GC-MS analyses in his laboratory. This work was financially supported by the National Natural Science Foundation of China (31991214) and the National Key Research and Development Program of China (Grant No. 2017YFD0101004).
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ZN and XC conceived the project; MZ provided glossy1 mutant; LL carried out experiments; LC, HX, and XC participated in field trials; QS, HP, YY, ZH, MX, MY, WG, HZ, and TW assisted in revising the manuscript; LL analyzed experimental results; LL and ZN wrote the manuscript.
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122_2020_3734_MOESM1_ESM.tif
Zoomed-in views of leaf blades (a), leaf sheaths (b) and stems (c) from Jimai22 (left) and the glossy1 mutant (right), respectively. Scale bar 1 cm. (TIF 1003 kb)
122_2020_3734_MOESM2_ESM.tif
Phenotypic characterization of the glossy1 mutant, an F1 plant, and the cultivar Jing 411. a Representative photograph of the glossy1 mutant (left), an F1 plant (middle) and Jing411 (right) at the flowering stage (GS65). Scale bar 10 cm; b Zoomed-in view of spikes from the glossy1 mutant (left), an F1 plant (middle), and Jing411 (right). Scale bar 1 cm. (TIF 1376 kb)
122_2020_3734_MOESM3_ESM.tif
Relative expression levels of five candidate genes in the glumes of Jimai22 and the glossy1 mutant. TaActin (TraesCS5D02G132200) was used as an internal reference. The bars represent standard deviation of the means, calculated from three biological replicates. The P value from a t-test between genotypes is shown above each gene. (TIF 59 kb)
122_2020_3734_MOESM4_ESM.tif
Comparison of the genomic positions of Iw2 and GLOSSY1. a Physical map of Iw2 according to the positions of Chinese Spring genes (Huang et al. 2017); b Corresponding physical positions are in Mbp on chromosome 2DS according to the Chinese Spring IWGSC RefSeq v1.1 sequence; c Physical map used for fine mapping of GLOSSY1. (TIF 77 kb)
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Li, L., Chai, L., Xu, H. et al. Phenotypic characterization of the glossy1 mutant and fine mapping of GLOSSY1 in common wheat (Triticum aestivum L.). Theor Appl Genet 134, 835–847 (2021). https://doi.org/10.1007/s00122-020-03734-6
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DOI: https://doi.org/10.1007/s00122-020-03734-6