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Evaluating the genetic basis of multiple-locule fruit in a broad cross section of tomato cultivars

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Abstract

Lycopersicon esculentum accessions bearing fasciated (multiloculed) fruit were characterized based on their flower organ and locule number phenotypes. Greenhouse and field evaluations indicate that increases in locule number are associated with increases in the number of other floral organs (e.g., sepals, petals, stamens) in all stocks. F1 complementation, F2 segregation analysis, and genetic mapping indicate that at least four loci account for increases in the number of carpels/locules in these stocks. The most significant of these map to the bottoms of chromosomes 2 and 11 and correspond to the locule number and fasciated loci. All stocks tested were fixed for mutations at the fasciated locus, which maps to the 0.5-cM interval between the markers T302 and cLET24J2A and occurs in at least three allelic forms (wild type and two mutants). One of the fasciated mutant alleles is associated with nonfused carpels and repressed recombination and may be due to a small inversion or deletion. The other two loci controlling locule number correspond to the lcn1.1 and lcn2.2 loci located on chromosomes 1 and 2, respectively.

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Acknowledgements

We thank Drs. E. van der Knaap and B. Cong for comments on the manuscript. We also thank C. de Sousa and N. van Eck for excellent technical assistance, and Dr. F. Vermeylen for help with the multidimensional scaling analysis. This work was supported by grants from the National Research Initiative Cooperative Grants Program, US Department of Agriculture Plant Genome Program (no. 0035300-9264); the National Science Foundation (no. 0116076); and the Binational Agricultural Research and Development Fund (no. IS-3009-980). L. Barrero was supported by a Fulbright–Colciencias scholarship.

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Authors and Affiliations

  1. Department of Plant Breeding, Cornell University, 245 Emerson Hall, Ithaca, NY, 14853-1902, USA

    L. S. Barrero

  2. Department of Plant Biology, Cornell University, 245 Emerson Hall, Ithaca, NY, 14853-1902, USA

    S. D. Tanksley

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  1. L. S. Barrero
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  2. S. D. Tanksley
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Correspondence to S. D. Tanksley.

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Communicated by G. Wenzel

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Barrero, L.S., Tanksley, S.D. Evaluating the genetic basis of multiple-locule fruit in a broad cross section of tomato cultivars. Theor Appl Genet 109, 669–679 (2004). https://doi.org/10.1007/s00122-004-1676-y

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