Abstract
In this paper, we introduce a novel method named chemiluminescence mediated sono dynamic diagnosis (SDD), which is designed as follows: sonosensitizer is used to localize the cancer tissue, and is sonosensitized by the ultrasound field to produce oxygen free radicals. Then, another agent, FCLA (fluoresceinyl cypridina luciferin analog), reacts with1O2 to efficiently transform the chemical energy of1O2 to photons, thus a strong chemiluminescence is emitted. One can then detect this emission with a high sensitive CCD imaging system to localize the tumor. Based on the principle of chemiluminescence mediated SDD, we obtained a clear diagnostic image of a transplanted tumor in a nude mouse in the experiments. This method could have potential applications in clinics for early stage tumor diagnosis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Tuchin, V. V., Handbook of Optical Biomedical Diagnosis, Bellingham, Washington USA: SPIE Press, 2002, 32–146.
Yumita, N., Nishigaki, R., Umemura, K. et al., Hematoporphyrin as sensitizer of cell-damaging effect of ultrasound, Jpn. J. Cancer Res., 1989, 80: 219–222.
Umemura, S., Yumita, N., Nishigaki, R. et al., Mechanism of cell damage by ultrasound in combination with hematoporphyrin, Jpn. J. Cancer Res., 1990, 81(9): 962–966.
Misik, V., Riesz, P., Free radical intermediates in sonodynamic therapy, Ann. N Y. Acad. Sci., 2000, 899: 335–348.
Miyoshi, N., Misik, V., Fukuda, M. et al., Effect of galliumporphyrin analogue ATX-70 on nitroxide formation from a cyclic secondary amine by ultrasound: on the mechanism of sonodynamic activation, Radiat. Res., 1995, 143: 194–202.
Qi, H., Tan, S. J., Ma, Y. Y. et al., Synergistic effects of focus ultrasound with different frequency and hematoporphyrin on human tumor cells, Science in China, Ser. C., 1998, 28(5): 477–480.
He, Y. H., Xing, D., Yan, G. H. et al., FCLA chemiluminescence from sonodynamic actionin vitro andin vivo, Cancer Letters, 2002, 182(2): 141–145.
Kessel, D., Jeffers, R., Fowlkes, B. et al., Porphyrin-induced enhancement of ultrasound cytotoxicity, Int. J. Radiat. Biol., 1994, 66(2): 221–228.
Wang, J., Xing, D., He, Y. H. et al., Experimental study on photodynamic diagnosis of cancer mediated by chemiluminescence probe, FEBS Letters, 2002, 523(1–3): 128–132.
He, Y. H., Xing, D., Tan, S. C. et al.,In vivo sonoluminescence imaging with the assistance of FCLA, Phys. Med. Biol., 2002, 47(9): 1535–1541.
Nakano, M., Sugioka, K., Ushuima, Y. et al., Chemiluminescence probe with cypridina luciferin analog estimating the ability of human granulocytes to generate O−2, Analytical Biochemistry, 1986, 159: 363–369.
Crum, L. A., Sonoluminescence, Physics Today, 1994, 47: 22–29.
Ritz, J. P., Roggan, A., Isbert, C. et al., Optical properties of native and coagulated porcine liver tissue between 400 and 2400 nm, Lasers Surg. Med., 2001, 29(3): 205–212.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
He, Y., Xing, D. & Wang, J. A novel cancer imaging method using chemiluminescence-mediated sonosensitization. Chin.Sci.Bull. 48, 777–780 (2003). https://doi.org/10.1007/BF03187051
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF03187051