Skip to main content
SpringerLink
Log in

Karyotype evolution in cell lines of Drosophila melanogaster

  • Published:
Chromosoma Aims and scope Submit manuscript

Abstract

The chromosomal changes occurred in two independent cell lines (GM2 and GM3) of Drosophila melanogaster maintained in medium supplemented with serum and in serum-free medium were compared. In both culture conditions and in both lines a chromosomal evolution was revealed. Structural and numerical variations were analysed. The breaks giving rise to rearrangements were at heterochromatic level. Moreover, a tetraploidisation followed by loss of chromosomes or of portions of chromosomes recalls an analogous cycle observed in human cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adkisson, K.P., Perreault, W.J., Gay, H.: Differential fluorescent staining of Drosophila chromosomes with quinacrine mustard. Chromosoma (Berl.) 34, 190–205 (1971)

    Google Scholar 

  • Dolfini, S.: Karyotype polymorphism in a cell population of Drosophila melanogaster cultured in vitro. Chromosoma (Berl.) 33, 196–208 (1971)

    Google Scholar 

  • Dolfini, S., Gottardi, A.: Changes of chromosome number in cells of Drosophila melanogaster cultured in vitro. Experientia (Basel) 22, 144 (1966)

    Google Scholar 

  • Echalier, G., Ohanessian, A.: In vitro culture of Drosophila melanogaster embryonic cells. In Vitro 6, 162–172 (1970)

    Google Scholar 

  • Ellison, J.R., Barr, H.J.: Difference in the quinacrine staining of the chromosomes of a pair of sibling species: Drosophila melanogaster and Drosophila simulans. Chromosoma (Berl.) 34, 424–435 (1971)

    Google Scholar 

  • Faccio Dolfini, S.: Spontaneous chromosome rearrangements in an established cell line of Drosophila melanogaster, Chromosoma (Berl.) 47, 253–261 (1974)

    Google Scholar 

  • Gatti, M., Tanzarella, C, Olivieri, G.: Analysis of the chromosome aberrations induced by X-rays in somatic cells of Drosophila melanogaster. Genetics 77, 701–719 (1974)

    Google Scholar 

  • Halfer, C., Tiepolo, L., Barigozzi, C., Fraccaro, M.: Timing of the DNA replication of translocated Y chromosome sections in somatic cells of Drosophila melanogaster. Chromosoma (Berl.) 27, 395–408 (1969)

    Google Scholar 

  • Horikawa, M., Fox, A.S.: Culture of embryonic cells of Drosophila melanogaster in vitro. Science 145, 1437–1439 (1964)

    Google Scholar 

  • Kaufmann, B.P.: Somatic mitoses of Drosophila melanogaster. J. Morph. 56, 125–155 (1934)

    Google Scholar 

  • Martin, G.M., Sprague, C.A.: Parasexual cycle in cultivated human somatic cells. Science 166, 761–763 (1969)

    Google Scholar 

  • Mosna, G.: Medium for Drosophila cells in vitro without serum. Dros. Inf. Serv. 49, 60 (1972)

    Google Scholar 

  • Mosna, G., Dolfini, S.: Morphological and chromosomal characterization of three new continuous cell lines of Drosophila melanogaster. Chromosoma (Berl.) 38, 1–9 (1972)

    Google Scholar 

  • Terzi, M., Hawkins, T.S.C.: Chromosomal variation and the establishment of somatic cell lines in vitro. Nature (Lond.) 253, 361–362 (1975)

    Google Scholar 

  • Vosa, C.G.: The discriminating fluorescence patterns of the chromosomes of Drosophila melanogaster. Chromosoma (Berl.) 31, 446–451 (1970)

    Google Scholar 

  • Zuffardi, O., Tiepolo, L., Dolfini, S., Barigozzi, C., Fraccaro, M.: Changes in the fluorescence patterns of translocated Y chromosome segments in Drosophila melanogaster. Chromosoma (Berl.) 34, 274–280 (1971)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Genetics, University of Milano, Via Celoria, 10, 20133, Milano, Italy

    Silvana Faccio Dolfini

Authors
  1. Silvana Faccio Dolfini
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by a grant of the Consiglio Nazionale delle Ricerche, Roma.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dolfini, S.F. Karyotype evolution in cell lines of Drosophila melanogaster . Chromosoma 58, 73–86 (1976). https://doi.org/10.1007/BF00293442

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00293442

Keywords

Search

Navigation