Alginate in Gene and Vaccine Delivery

Abdelhamid, Hani Nasser

doi:10.1007/978-981-19-6937-9_14

Hani Nasser Abdelhamid^3,4,5

441 Accesses

Abstract

Gene and vaccine delivery offer promising technologies for the treatment of several diseases. However, they suffer from low transfection efficacy, enzyme degradation, and immunogenicity of the gene-based therapeutic agents or vaccines. Thus, several materials were reported as carriers for gene therapeutic agents. Alginates-based materials provide solutions for several challenges of other biomaterials. They exhibit high biocompatibility for many biological cells with low or minimal toxicity, show high stability under different environments, and can proceed quickly into various forms such as beads, capsules, fibers, and hydrogels. Bio-beads of calcium alginate is widely used to encapsulate gene-based therapeutic agents. They can be quickly processed as three-dimensional (3D) scaffolds, hydrogels, capsules, spheres, foams, sponges, and fibers. They can be used as carriers for gene and vaccine delivery. They offer several advantages, such as high biodegradability, good encapsulation efficiency, excellent biocompatibility, and good chelating capacity. The alginate-based system was used for gene delivery for tissue generation, bone generation, cartilage repair, and cancer therapy. Alginate-based biomaterials offered the development of gene-activated bio-inks (GABs) for 3D printing. This book chapter summarizes the applications of alginate as carriers for gene and vaccine delivery.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Chapter: USD 29.95; Price excludes VAT (USA)

eBook: USD 189.00; Price excludes VAT (USA)

Softcover Book: USD 249.99; Price excludes VAT (USA)

Hardcover Book: USD 249.99; Price excludes VAT (USA)

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

Abdelhamid HN, Dowaidar M, Hällbrink M, Langel Ü (2019) Cell penetrating peptides-hierarchical porous zeolitic imidazolate frameworks nanoparticles: an efficient gene delivery platform. SSRN Electron J. https://doi.org/10.2139/ssrn.3435895
Abdelhamid HN, Dowaidar M, Langel Ü (2020) Carbonized chitosan encapsulated hierarchical porous zeolitic imidazolate frameworks nanoparticles for gene delivery. Microporous Mesoporous Mater 302:110200. https://doi.org/10.1016/j.micromeso.2020.110200
Article CAS Google Scholar
Ahmad Zamri N, Rusli MEF, Mohamad Yusof L, Rosli R (2022) Immunization with a bicistronic DNA vaccine modulates systemic IFN-γ and IL-10 expression against Vibrio cholerae infection. J Med Microbiol 71. https://doi.org/10.1099/jmm.0.001536
Augst AD, Kong HJ, Mooney DJ (2006) Alginate hydrogels as biomaterials. Macromol Biosci 6:623–633. https://doi.org/10.1002/mabi.200600069
Article CAS Google Scholar
Aynié I, Vauthier C, Chacun H, Fattal E, Couvreur P (1999) Spongelike alginate nanoparticles as a new potential system for the delivery of antisense oligonucleotides. Antisense Nucleic Acid Drug Dev 9:301–312. https://doi.org/10.1089/oli.1.1999.9.301
Article Google Scholar
Azadi SA, Vasheghani-Farahani E, Hashemi-Najafbabadi S, Godini A (2016) Co-encapsulation of pancreatic islets and pentoxifylline in alginate-based microcapsules with enhanced immunosuppressive effects. Prog Biomater 5:101–109. https://doi.org/10.1007/s40204-016-0049-3
Article CAS Google Scholar
Bagheri-Josheghani S, Bakhshi B (2022) Formulation of selenium nanoparticles encapsulated by alginate-chitosan for controlled delivery of vibrio cholerae LPS: a novel delivery system candidate for nanovaccine. Int J Biol Macromol 208:494–508. https://doi.org/10.1016/j.ijbiomac.2022.03.087
Article CAS Google Scholar
Bagre AP, Jain K, Jain NK (2013) Alginate coated chitosan core shell nanoparticles for oral delivery of enoxaparin: in vitro and in vivo assessment. Int J Pharm 456:31–40. https://doi.org/10.1016/j.ijpharm.2013.08.037
Article CAS Google Scholar
Ballesteros NA, Alonso M, Saint-Jean SR, Perez-Prieto SI (2015) An oral DNA vaccine against infectious haematopoietic necrosis virus (IHNV) encapsulated in alginate microspheres induces dose-dependent immune responses and significant protection in rainbow trout (Oncorrhynchus mykiss). Fish Shellfish Immunol 45:877–888. https://doi.org/10.1016/j.fsi.2015.05.045
Article CAS Google Scholar
Bedell ML, Navara AM, Du Y, Zhang S, Mikos AG (2020) Polymeric systems for bioprinting. Chem Rev 120:10744–10792. https://doi.org/10.1021/acs.chemrev.9b00834
Article CAS Google Scholar
Bi Y, Lin Z, Deng S (2019) Fabrication and characterization of hydroxyapatite/sodium alginate/chitosan composite microspheres for drug delivery and bone tissue engineering. Mater Sci Eng C 100:576–583. https://doi.org/10.1016/j.msec.2019.03.040
Article CAS Google Scholar
Bøgwald J, Dalmo RA (2021) Protection of teleost fish against infectious diseases through oral administration of vaccines: update 2021. Int J Mol Sci 22:10932. https://doi.org/10.3390/ijms222010932
Article CAS Google Scholar
Boisguérin P, Deshayes S, Gait MJ, O’Donovan L, Godfrey C, Betts CA et al (2015) delivery of therapeutic oligonucleotides with cell penetrating peptides. Adv Drug Deliv Rev 87:52–67. https://doi.org/10.1016/j.addr.2015.02.008
Article CAS Google Scholar
Boontheekul T, Kong H-J, Mooney DJ (2005) Controlling alginate gel degradation utilizing partial oxidation and bimodal molecular weight distribution. Biomaterials 26:2455–2465. https://doi.org/10.1016/j.biomaterials.2004.06.044
Article CAS Google Scholar
Bouhadir KH, Lee KY, Alsberg E, Damm KL, Anderson KW, Mooney DJ (2001) Degradation of partially oxidized alginate and its potential application for tissue engineering. Biotechnol Prog 17:945–950. https://doi.org/10.1021/bp010070p
Article CAS Google Scholar
Bowersock T, Hogenesch H, Suckow M, Guimond P, Martin S, Borie D et al (1999) Oral vaccination of animals with antigens encapsulated in alginate microspheres. Vaccine 17:1804–1811. https://doi.org/10.1016/S0264-410X(98)00437-X
Article CAS Google Scholar
Bozo IY, Mavlikeev MO, Presnyakov EV, Yasinovsky MI, Trofimov VO, Indeykin FA et al (2021) Gene-activated hydrogels based on sodium alginate for reparative myogenesis of skeletal muscle. Inorg Mater Appl Res 12:1026–1032. https://doi.org/10.1134/S2075113321040092
Article Google Scholar
Bucher C, Gazdhar A, Benneker LM, Geiser T, Gantenbein-Ritter B (2013) Nonviral gene delivery of growth and differentiation factor 5 to human mesenchymal stem cells injected into a 3D bovine intervertebral disc organ culture system. Stem Cells Int 2013:1–9. https://doi.org/10.1155/2013/326828
Article Google Scholar
Byambaa B, Annabi N, Yue K, Trujillo-de Santiago G, Alvarez MM, Jia W et al (2017) Bioprinted osteogenic and vasculogenic patterns for engineering 3D bone tissue. Adv Healthc Mater 6:1700015. https://doi.org/10.1002/adhm.201700015
Article CAS Google Scholar
Cao L, Lu W, Mata A, Nishinari K, Fang Y (2020a) Egg-box model-based gelation of alginate and pectin: a review. Carbohydr Polym 242:116389. https://doi.org/10.1016/j.carbpol.2020.116389
Article CAS Google Scholar
Cao P, Han FY, Grøndahl L, Xu ZP, Li L (2020b) Enhanced oral vaccine efficacy of polysaccharide-coated calcium phosphate nanoparticles. ACS Omega 5:18185–18197. https://doi.org/10.1021/acsomega.0c01792
Article CAS Google Scholar
Chang TMS (1992) Artificial cells in immobilization biotechnology. Biomater Artif Cells Immobil Biotechnol 20:1121–1143. https://doi.org/10.3109/10731199209117340
Article CAS Google Scholar
Choe G, Oh S, Seok JM, Park SA, Lee JY (2019) Graphene oxide/alginate composites as novel bioinks for three-dimensional mesenchymal stem cell printing and bone regeneration applications. Nanoscale 11:23275–23285. https://doi.org/10.1039/C9NR07643C
Article CAS Google Scholar
Choi Y, Park K, Choi H, Son D, Shin M (2021) Self-healing, stretchable, biocompatible, and conductive alginate hydrogels through dynamic covalent bonds for implantable electronics. Polymers (Basel) 13:1133. https://doi.org/10.3390/polym13071133
Article CAS Google Scholar
Chueh B, Zheng Y, Torisawa Y, Hsiao AY, Ge C, Hsiong S et al (2010) Patterning alginate hydrogels using light-directed release of caged calcium in a microfluidic device. Biomed Microdevices 12:145–151. https://doi.org/10.1007/s10544-009-9369-6
Article Google Scholar
Coleman RJ, Lawrie G, Lambert LK, Whittaker M, Jack KS, Grøndahl L (2011) Phosphorylation of alginate: synthesis, characterization, and evaluation of in vitro mineralization capacity. Biomacromolecules 12:889–897. https://doi.org/10.1021/bm1011773
Article CAS Google Scholar
Cui J, Wang M, Zheng Y, Rodríguez Muñiz GM, del Campo A (2013) Light-triggered cross-linking of alginates with caged Ca²⁺. Biomacromolecules 14:1251–1256. https://doi.org/10.1021/bm400022h
Article CAS Google Scholar
Cunniffe GM, Gonzalez-Fernandez T, Daly A, Sathy BN, Jeon O, Alsberg E et al (2017) Three-dimensional bioprinting of polycaprolactone reinforced gene activated bioinks for bone tissue engineering. Tissue Eng Part A 23:891–900. https://doi.org/10.1089/ten.tea.2016.0498
Article CAS Google Scholar
Danielsen S, Maurstad G, Stokke BT (2005) DNA-polycation complexation and polyplex stability in the presence of competing polyanions. Biopolymers 77:86–97. https://doi.org/10.1002/bip.20170
Article CAS Google Scholar
Dey P, Ramanujam R, Venkatesan G, Nagarathnam R (2019) Sodium alginate potentiates antioxidant defense and PR proteins against early blight disease caused by Alternaria solani in Solanum lycopersicum Linn. PLoS One 14:e0223216. https://doi.org/10.1371/journal.pone.0223216
Article CAS Google Scholar
Diniz FR, Maia RCAP, Rannier Andrade L, Andrade LN, Vinicius Chaud M, da Silva CF et al (2020) Silver nanoparticles-composing alginate/gelatine hydrogel improves wound healing in vivo. Nano 10:390. https://doi.org/10.3390/nano10020390
Article CAS Google Scholar
Dodero A, Alberti S, Gaggero G, Ferretti M, Botter R, Vicini S et al (2021) An up-to-date review on alginate nanoparticles and nanofibers for biomedical and pharmaceutical applications. Adv Mater Interfaces 8:2100809. https://doi.org/10.1002/admi.202100809
Article CAS Google Scholar
Douglas KL, Tabrizian M (2005) Effect of experimental parameters on the formation of alginate–chitosan nanoparticles and evaluation of their potential application as DNA carrier. J Biomater Sci Polym Ed 16:43–56. https://doi.org/10.1163/1568562052843339
Article CAS Google Scholar
Douglas KL, Piccirillo CA, Tabrizian M (2006) Effects of alginate inclusion on the vector properties of chitosan-based nanoparticles. J Control Release 115:354–361. https://doi.org/10.1016/j.jconrel.2006.08.021
Article CAS Google Scholar
Dowaidar M, Abdelhamid HN, Hällbrink M, Zou X, Langel Ü (1861) Graphene oxide nanosheets in complex with cell penetrating peptides for oligonucleotides delivery. Biochim Biophys Acta Gen Subj 2017:2334–2341. https://doi.org/10.1016/j.bbagen.2017.07.002
Article CAS Google Scholar
Dowaidar M, Abdelhamid HN, Hällbrink M, Kurrikoff K, Freimann K, Zou X et al (2017) Magnetic nanoparticle assisted self-assembly of cell penetrating peptides-oligonucleotides complexes for gene delivery. Sci Rep 7:9159. https://doi.org/10.1038/s41598-017-09803-z
Article CAS Google Scholar
Dowaidar M, Nasser Abdelhamid H, Hällbrink M, Langel Ü, Zou X (2018) Chitosan enhances gene delivery of oligonucleotide complexes with magnetic nanoparticles–cell-penetrating peptide. J Biomater Appl 33:392–401. https://doi.org/10.1177/0885328218796623
Article CAS Google Scholar
Dowaidar M, Abdelhamid HN, Langel Ü (2022) Improvement of transfection with pepfects using organic and inorganic materials. Methods Mol Biol 83:555–567. https://doi.org/10.1007/978-1-0716-1752-6_35
Article Google Scholar
Drury JL, Dennis RG, Mooney DJ (2004) The tensile properties of alginate hydrogels. Biomaterials 25:3187–3199. https://doi.org/10.1016/j.biomaterials.2003.10.002
Article CAS Google Scholar
Dvir-Ginzberg M, Gamlieli-Bonshtein I, Agbaria R, Cohen S (2003) Liver tissue engineering within alginate scaffolds: effects of cell-seeding density on hepatocyte viability, morphology, and function. Tissue Eng 9:757–766. https://doi.org/10.1089/107632703768247430
Article CAS Google Scholar
Eiselt P, Lee KY, Mooney DJ (1999) Rigidity of two-component hydrogels prepared from alginate and poly(ethylene glycol)−diamines. Macromolecules 32:5561–5566. https://doi.org/10.1021/ma990514m
Article CAS Google Scholar
Eiselt P, Yeh J, Latvala RK, Shea LD, Mooney DJ (2000) Porous carriers for biomedical applications based on alginate hydrogels. Biomaterials 21:1921–1927. https://doi.org/10.1016/S0142-9612(00)00033-8
Article CAS Google Scholar
Farokhi M, Jonidi Shariatzadeh F, Solouk A, Mirzadeh H (2020) Alginate based scaffolds for cartilage tissue engineering: a review. Int J Polym Mater Polym Biomater 69:230–247. https://doi.org/10.1080/00914037.2018.1562924
Article CAS Google Scholar
Franck CO, Fanslau L, Bistrovic Popov A, Tyagi P, Fruk L (2021) Biopolymer-based carriers for DNA vaccine design. Angew Chemie Int Ed Engl 60:13225–13243. https://doi.org/10.1002/anie.202010282
Article CAS Google Scholar
Freudenberger Catanzaro KC, Lahmers KK, Allen IC, Inzana TJ (2022) Alginate microencapsulation of an attenuated O-antigen mutant of Francisella tularensis LVS as a model for a vaccine delivery vehicle. PLoS One 17:e0259807. https://doi.org/10.1371/journal.pone.0259807
Article CAS Google Scholar
Furuya DC, da Costa SA, de Oliveira RC, Ferraz HG, Pessoa Junior A, da Costa SM (2017) Fibers obtained from alginate, chitosan and hybrid used in the development of scaffolds. Mater Res 20:377–386. https://doi.org/10.1590/1980-5373-mr-2016-0509
Article CAS Google Scholar
Gaafar MR, El-Mansoury ST, Eissa MM, Shalaby TI, Younis LK, Rashed HA (2022) Effect of alginate nanoparticles on the immunogenicity of excretory-secretory antigens against acute toxoplasmosis in murine model. Acta Trop 225:106215. https://doi.org/10.1016/j.actatropica.2021.106215
Article CAS Google Scholar
Gacesa P (1998) Bacterial alginate biosynthesis—recent progress and future prospects. Microbiology 144:1133–1143. https://doi.org/10.1099/00221287-144-5-1133
Article CAS Google Scholar
Gonzalez-Fernandez T, Kelly DJ, O’Brien FJ (2018) Controlled non-viral gene delivery in cartilage and bone repair: current strategies and future directions. Adv Ther 1:1800038. https://doi.org/10.1002/adtp.201800038
Article Google Scholar
Gonzalez-Fernandez T, Rathan S, Hobbs C, Pitacco P, Freeman FE, Cunniffe GM et al (2019) Pore-forming bioinks to enable spatio-temporally defined gene delivery in bioprinted tissues. J Control Release 301:13–27. https://doi.org/10.1016/j.jconrel.2019.03.006
Article CAS Google Scholar
Gopal J, Abdelhamid HN, Hua P-Y, Wu H-F (2013) Chitosan nanomagnets for effective extraction and sensitive mass spectrometric detection of pathogenic bacterial endotoxin from human urine. J Mater Chem B 1:2463. https://doi.org/10.1039/c3tb20079e
Article CAS Google Scholar
Guastaferro M, Reverchon E, Baldino L (2021) Agarose, alginate and chitosan nanostructured aerogels for pharmaceutical applications: a short review. Front Bioeng Biotechnol 9:688477. https://doi.org/10.3389/fbioe.2021.688477
Article Google Scholar
Gurikov P, Raman SP, Weinrich D, Fricke M, Smirnova I (2015) A novel approach to alginate aerogels: carbon dioxide induced gelation. RSC Adv 5:7812–7818. https://doi.org/10.1039/C4RA14653K
Article CAS Google Scholar
Haji Ali Babaei R, Shapoori R, Beig M (2022) Evaluation of alginate-exotoxin a conjugated vaccine immunogenicity against pseudomonas aeruginosa infection in mouse model. Iran J Med Microbiol 16:1–16. https://doi.org/10.30699/ijmm.16.1.1
Article Google Scholar
Halimi M, Alishahi M, Abbaspour MR, Ghorbanpoor M, Tabandeh MR (2019) Valuable method for production of oral vaccine by using alginate and chitosan against lactococcus garvieae/streptococcus iniae in rainbow trout (oncorhynchus mykiss). Fish Shellfish Immunol 90:431–439. https://doi.org/10.1016/j.fsi.2019.05.020
Article CAS Google Scholar
Hao Y, Zhao W, Zhang L, Zeng X, Sun Z, Zhang D et al (2020) Bio-multifunctional alginate/chitosan/fucoidan sponges with enhanced angiogenesis and hair follicle regeneration for promoting full-thickness wound healing. Mater Des 193:108863. https://doi.org/10.1016/j.matdes.2020.108863
Article CAS Google Scholar
Hay ID, Ur Rehman Z, Ghafoor A, Rehm BHA (2010) Bacterial biosynthesis of alginates. J Chem Technol Biotechnol 85:752–759. https://doi.org/10.1002/jctb.2372
Article CAS Google Scholar
He W, Guo Z, Wen Y, Wang Q, Xie B, Zhu S et al (2012) Alginate-graft-PEI as a gene delivery vector with high efficiency and low cytotoxicity. J Biomater Sci Polym Ed 23:315–331. https://doi.org/10.1163/092050610X550359
Article CAS Google Scholar
Heid S, Boccaccini AR (2020) Advancing bioinks for 3D bioprinting using reactive fillers: a review. Acta Biomater 113:1–22. https://doi.org/10.1016/j.actbio.2020.06.040
Article CAS Google Scholar
Helmiyati AM (2017) Characterization and properties of sodium alginate from brown algae used as an ecofriendly superabsorbent. IOP Conf Ser Mater Sci Eng 188:012019. https://doi.org/10.1088/1757-899X/188/1/012019
Article Google Scholar
Hu X, Yang J, Chen C, Khan H, Guo Y, Yang L (2018a) Capillary electrophoresis-integrated immobilized enzyme microreactor utilizing single-step in-situ penicillinase-mediated alginate hydrogelation: application for enzyme assays of penicillinase. Talanta 189:377–382. https://doi.org/10.1016/j.talanta.2018.07.019
Article CAS Google Scholar
Hu W-W, Wu Y-C, Hu Z-C (2018b) The development of an alginate/polycaprolactone composite scaffold for in situ transfection application. Carbohydr Polym 183:29–36. https://doi.org/10.1016/j.carbpol.2017.11.030
Article CAS Google Scholar
Huang X, Ma Y, Wang Y, Niu C, Liu Z, Yao X et al (2021) Oral probiotic vaccine expressing Koi Herpesvirus (KHV) ORF81 protein delivered by chitosan-alginate capsules is a promising strategy for mass oral vaccination of carps against KHV infection. J Virol 95:e00415–e00421. https://doi.org/10.1128/JVI.00415-21
Article CAS Google Scholar
Javvaji V, Baradwaj AG, Payne GF, Raghavan SR (2011) Light-activated ionic gelation of common biopolymers. Langmuir 27:12591–12596. https://doi.org/10.1021/la201860s
Article CAS Google Scholar
Jazayeri SD, Lim HX, Shameli K, Yeap SK, Poh CL (2021) Nano and microparticles as potential oral vaccine carriers and adjuvants against infectious diseases. Front Pharmacol 12:682286. https://doi.org/10.3389/fphar.2021.682286
Article CAS Google Scholar
Jiang L, Lu Y, Liu X, Tu H, Zhang J, Shi X et al (2015) Layer-by-layer immobilization of quaternized carboxymethyl chitosan/organic rectorite and alginate onto nanofibrous mats and their antibacterial application. Carbohydr Polym 121:428–435. https://doi.org/10.1016/j.carbpol.2014.12.069
Article CAS Google Scholar
Kanasan N, Adzila S, Azimah Mustaffa N, Gurubaran P (2017) The effect of sodium alginate on the properties of hydroxyapatite. Procedia Eng 184:442–448. https://doi.org/10.1016/j.proeng.2017.04.115
Article CAS Google Scholar
Kesarwani A, Sahu P, Jain K, Sinha P, Mohan KV, Nagpal PS et al (2021) The safety and efficacy of BCG encapsulated alginate particle (BEAP) against M.tb H37Rv infection in macaca mulatta: a pilot study. Sci Rep 11:3049. https://doi.org/10.1038/s41598-021-82614-5
Article CAS Google Scholar
Khvorostina MA, Mironov AV, Nedorubova IA, Bukharova TB, Vasilyev AV, Goldshtein DV et al (2022) 3D printed gene-activated sodium alginate hydrogel scaffolds. Gels 8:421. https://doi.org/10.3390/gels8070421
Article CAS Google Scholar
Kilian D, Ahlfeld T, Akkineni AR, Bernhardt A, Gelinsky M, Lode A (2020) 3D bioprinting of osteochondral tissue substitutes—in vitro-chondrogenesis in multi-layered mineralized constructs. Sci Rep 10:8277. https://doi.org/10.1038/s41598-020-65050-9
Article CAS Google Scholar
Kong H (2003) Designing alginate hydrogels to maintain viability of immobilized cells. Biomaterials 24:4023–4029. https://doi.org/10.1016/S0142-9612(03)00295-3
Article CAS Google Scholar
Kong HJ, Mooney DJ (2003) The effects of poly(ethyleneimine) (PEI) molecular weight on reinforcement of alginate hydrogels. Cell Transplant 12:779–785. https://doi.org/10.3727/000000003108747253
Article Google Scholar
Krebs MD, Jeon O, Alsberg E (2009) Localized and sustained delivery of silencing RNA from macroscopic biopolymer hydrogels. J Am Chem Soc 131:9204–9206. https://doi.org/10.1021/ja9037615
Article CAS Google Scholar
Krebs MD, Salter E, Chen E, Sutter KA, Alsberg E (2010) Calcium phosphate-DNA nanoparticle gene delivery from alginate hydrogels induces in vivo osteogenesis. J Biomed Mater Res A 92(3):1131–1138. https://doi.org/10.1002/jbm.a.32441
Article CAS Google Scholar
Lakkakula JR, Gujarathi P, Pansare P, Tripathi S (2021) A comprehensive review on alginate-based delivery systems for the delivery of chemotherapeutic agent: doxorubicin. Carbohydr Polym 259:117696. https://doi.org/10.1016/j.carbpol.2021.117696
Article CAS Google Scholar
Lee KY, Mooney DJ (2012) Alginate: properties and biomedical applications. Prog Polym Sci 37:106–126. https://doi.org/10.1016/j.progpolymsci.2011.06.003
Article CAS Google Scholar
Lee CSD, Moyer HR, Gittens RAI, Williams JK, Boskey AL, Boyan BD et al (2010) Regulating in vivo calcification of alginate microbeads. Biomaterials 31:4926–4934. https://doi.org/10.1016/j.biomaterials.2010.03.001
Article CAS Google Scholar
LeRoux MA, Guilak F, Setton LA (1999) Compressive and shear properties of alginate gel: effects of sodium ions and alginate concentration. J Biomed Mater Res 47:46–53. https://doi.org/10.1002/(SICI)1097-4636(199910)47:1<46::AID-JBM6>3.0.CO;2-N
Article CAS Google Scholar
Leslie SK, Nicolini AM, Sundaresan G, Zweit J, Boyan BD, Schwartz Z (2016) Development of a cell delivery system using alginate microbeads for tissue regeneration. J Mater Chem B 4:3515–3525. https://doi.org/10.1039/C6TB00035E
Article CAS Google Scholar
Leung KC-F, Sham KWY, Chak C-P, Lai JMY, Lee S-F, Wáng Y-XJ et al (2015) Evaluation of biocompatible alginate- and deferoxamine-coated ternary composites for magnetic resonance imaging and gene delivery into glioblastoma cells. Quant Imaging Med Surg 5:382–391. https://doi.org/10.3978/j.issn.2223-4292.2015.03.12
Article Google Scholar
Li D, Zhuang J, He H, Jiang S, Banerjee A, Lu Y et al (2017) Influence of particle geometry on gastrointestinal transit and absorption following oral administration. ACS Appl Mater Interfaces 9:42492–42502. https://doi.org/10.1021/acsami.7b11821
Article CAS Google Scholar
Liu Z, Lv D, Liu S, Gong J, Wang D, Xiong M et al (2013) Alginic acid-coated chitosan nanoparticles loaded with legumain DNA vaccine: effect against breast cancer in mice. PLoS One 8:e60190. https://doi.org/10.1371/journal.pone.0060190
Article CAS Google Scholar
Liu G, Zhou H, Wu H, Chen R, Guo S (2016) Preparation of alginate hydrogels through solution extrusion and the release behavior of different drugs. J Biomater Sci Polym Ed 27:1808–1823. https://doi.org/10.1080/09205063.2016.1237452
Article CAS Google Scholar
Liu X, Liu H, Qu X, Lei M, Zhang C, Hong H et al (2017) Electrical signals triggered controllable formation of calcium-alginate film for wound treatment. J Mater Sci Mater Med 28:146. https://doi.org/10.1007/s10856-017-5956-x
Article CAS Google Scholar
Liu W, Madry H, Cucchiarini M (2022) Application of alginate hydrogels for next-generation articular cartilage regeneration. Int J Mol Sci 23:1147. https://doi.org/10.3390/ijms23031147
Article CAS Google Scholar
Loozen LD, van der Helm YJM, Öner FC, Dhert WJA, Kruyt MC, Alblas J (2015) Bone morphogenetic protein-2 nonviral gene therapy in a goat iliac crest model for bone formation. Tissue Eng Part A 21:1672–1679. https://doi.org/10.1089/ten.tea.2014.0593
Article CAS Google Scholar
Mahlicli FY, Altinkaya SA (2013) Surface modification of polysulfone based hemodialysis membranes with layer by layer self assembly of polyethyleneimine/alginate-heparin: a simple polyelectrolyte blend approach for heparin immobilization. J Mater Sci Mater Med 24:533–546. https://doi.org/10.1007/s10856-012-4804-2
Article CAS Google Scholar
Maity C, Das N (2022) Alginate-based smart materials and their application: recent advances and perspectives. Top Curr Chem 380:3. https://doi.org/10.1007/s41061-021-00360-8
Article CAS Google Scholar
Mallakpour S, Azadi E, Hussain CM (2021) State-of-the-art of 3D printing technology of alginate-based hydrogels—an emerging technique for industrial applications. Adv Colloid Interf Sci 293:102436. https://doi.org/10.1016/j.cis.2021.102436
Article CAS Google Scholar
Mazza G, Al-Akkad W, Rombouts K, Pinzani M (2018) Liver tissue engineering: from implantable tissue to whole organ engineering. Hepatol Commun 2:131–141. https://doi.org/10.1002/hep4.1136
Article Google Scholar
Moe ST, Skjåk-Bræk G, Smidsrød O (1991) Covalently cross-linked sodium alginate beads. Food Hydrocoll 5:119–123. https://doi.org/10.1016/S0268-005X(09)80296-8
Article CAS Google Scholar
Mørch ÝA, Donati I, Strand BL, Skjåk-Bræk G (2006) Effect of Ca²⁺, Ba²⁺, and Sr²⁺ on alginate microbeads. Biomacromolecules 7:1471–1480. https://doi.org/10.1021/bm060010d
Article CAS Google Scholar
Mosahebi A, Wiberg M, Terenghi G (2003) Addition of fibronectin to alginate matrix improves peripheral nerve regeneration in tissue-engineered conduits. Tissue Eng 9:209–218. https://doi.org/10.1089/107632703764664684
Article CAS Google Scholar
Najafi A, Ghazvini K, Sankian M, Gholami L, Amini Y, Zare S et al (2021) T helper type 1 biased immune responses by PPE17 loaded core-shell alginate-chitosan nanoparticles after subcutaneous and intranasal administration. Life Sci 282:119806. https://doi.org/10.1016/j.lfs.2021.119806
Article CAS Google Scholar
Niculescu A-G, Grumezescu AM (2022) Applications of chitosan-alginate-based nanoparticles—an up-to-date review. Nano 12:186. https://doi.org/10.3390/nano12020186
Article CAS Google Scholar
Niidome T, Huang L (2002) Gene therapy progress and prospects: non-viral vectors. Gene Ther 9:1647–1652. https://doi.org/10.1038/sj.gt.3301923
Article CAS Google Scholar
Nograles N, Abdullah S, Shamsudin MN, Billa N, Rosli R (2012) Formation and characterization of pDNA-loaded alginate microspheres for oral administration in mice. J Biosci Bioeng 113:133–140. https://doi.org/10.1016/j.jbiosc.2011.10.003
Article CAS Google Scholar
Núñez C, López-Pliego L, Ahumada-Manuel CL, Castañeda M (2022) Genetic regulation of alginate production in azotobacter vinelandii a bacterium of biotechnological interest: a mini-review. Front Microbiol 13:845473. https://doi.org/10.3389/fmicb.2022.845473
Article Google Scholar
O’Brien FJ (2011) Biomaterials & scaffolds for tissue engineering. Mater Today 14:88–95. https://doi.org/10.1016/S1369-7021(11)70058-X
Article CAS Google Scholar
Oh H, Lu AX, Javvaji V, DeVoe DL, Raghavan SR (2016) Light-directed self-assembly of robust alginate gels at precise locations in microfluidic channels. ACS Appl Mater Interfaces 8:17529–17538. https://doi.org/10.1021/acsami.6b03826
Article CAS Google Scholar
Onuigbo E, Iseghohimhen J, Chah K, Gyang M, Attama A (2018) Chitosan/alginate microparticles for the oral delivery of fowl typhoid vaccine: Innate and acquired immunity. Vaccine 36:4973–4978. https://doi.org/10.1016/j.vaccine.2018.05.087
Article CAS Google Scholar
Otto DP, de Villiers MM (2020) Layer-by-layer nanocoating of antiviral polysaccharides on surfaces to prevent coronavirus infections. Molecules 25:3415. https://doi.org/10.3390/molecules25153415
Article CAS Google Scholar
Paques JP, van der Linden E, van Rijn CJM, Sagis LMC (2014) Preparation methods of alginate nanoparticles. Adv Colloid Interf Sci 209:163–171. https://doi.org/10.1016/j.cis.2014.03.009
Article CAS Google Scholar
Pardee KI, Ellis P, Bouthillier M, Towers GH, French CJ (2004) Plant virus inhibitors from marine algae. Can J Bot 82:304–309. https://doi.org/10.1139/b04-002
Article Google Scholar
Pawar SN, Edgar KJ (2011) Chemical modification of alginates in organic solvent systems. Biomacromolecules 12:4095–4103. https://doi.org/10.1021/bm201152a
Article CAS Google Scholar
Pawar SN, Edgar KJ (2012) Alginate derivatization: a review of chemistry, properties and applications. Biomaterials 33:3279–3305. https://doi.org/10.1016/j.biomaterials.2012.01.007
Article CAS Google Scholar
Pietropaolo V, Seganti L, Marchetti M, Sinibaldi L, Orsi N, Nicoletti R (1993) Effect of natural and semisynthetic polymers on rabies virus infection in CER cells. Res Virol 144:151–158. https://doi.org/10.1016/S0923-2516(06)80023-3
Article CAS Google Scholar
Plotkin SA (2005) Vaccines: past, present and future. Nat Med 11:S5–S11. https://doi.org/10.1038/nm1209
Article CAS Google Scholar
Prang P, Muller R, Eljaouhari A, Heckmann K, Kunz W, Weber T et al (2006) The promotion of oriented axonal regrowth in the injured spinal cord by alginate-based anisotropic capillary hydrogels. Biomaterials 27(19):3560–3569. https://doi.org/10.1016/j.biomaterials.2006.01.053
Article CAS Google Scholar
Raguavaran R, Mondal DB, Sharma DK, Jithin MV, Yadav N (2022) Biomedical applications of alginate nanoparticles. In: Venkatesan J, Kim S-K, Anil S, Rekha PD (eds) Polysaccharide nanoparticles. Elsevier, Amsterdam, pp 311–328. https://doi.org/10.1016/B978-0-12-822351-2.00002-4
Chapter Google Scholar
Reakasame S, Boccaccini AR (2018) Oxidized alginate-based hydrogels for tissue engineering applications: a review. Biomacromolecules 19:3–21. https://doi.org/10.1021/acs.biomac.7b01331
Article CAS Google Scholar
Reig-Vano B, Tylkowski B, Montané X, Giamberini M (2021) Alginate-based hydrogels for cancer therapy and research. Int J Biol Macromol 170:424–436. https://doi.org/10.1016/j.ijbiomac.2020.12.161
Article CAS Google Scholar
Remminghorst U, Rehm BHA (2006) Bacterial alginates: from biosynthesis to applications. Biotechnol Lett 28:1701–1712. https://doi.org/10.1007/s10529-006-9156-x
Article CAS Google Scholar
Rocha CEV, Silva MF, Guedes ACB, Carvalho TP, Eckstein C, Ribeiro NQ et al (2021) Alginate-chitosan microcapsules improve vaccine potential of gamma-irradiated listeria monocytogenes against listeriosis in murine model. Int J Biol Macromol 176:567–577. https://doi.org/10.1016/j.ijbiomac.2021.02.056
Article CAS Google Scholar
Romalde JL, Luzardo-Alvárez A, Ravelo C, Toranzo AE, Blanco-Méndez J (2004) Oral immunization using alginate microparticles as a useful strategy for booster vaccination against fish lactoccocosis. Aquaculture 236:119–129. https://doi.org/10.1016/j.aquaculture.2004.02.028
Article CAS Google Scholar
Ronghua H, Yumin D, Jianhong Y (2003) Preparation and in vitro anticoagulant activities of alginate sulfate and its quaterized derivatives. Carbohydr Polym 52:19–24. https://doi.org/10.1016/S0144-8617(02)00258-8
Article Google Scholar
Sahoo DR, Biswal T (2021) Alginate and its application to tissue engineering. SN Appl Sci 3:30. https://doi.org/10.1007/s42452-020-04096-w
Article CAS Google Scholar
Serrano-Aroca Á, Ferrandis-Montesinos M, Wang R (2021) Antiviral properties of alginate-based biomaterials: promising antiviral agents against SARS-CoV-2. ACS Appl Bio Mater 4:5897–5907. https://doi.org/10.1021/acsabm.1c00523
Article CAS Google Scholar
Shao F, Yu L, Zhang Y, An C, Zhang H, Zhang Y et al (2020) Microfluidic encapsulation of single cells by alginate microgels using a trigger-gellified strategy. Front Bioeng Biotechnol 8:583065. https://doi.org/10.3389/fbioe.2020.583065
Article Google Scholar
Siew CK, Williams PA, Young NWG (2005) New insights into the mechanism of gelation of alginate and pectin: charge annihilation and reversal mechanism. Biomacromolecules 6:963–969. https://doi.org/10.1021/bm049341l
Article CAS Google Scholar
Skjåk-Bræk G, Zanetti F, Paoletti S (1989) Effect of acetylation on some solution and gelling properties of alginates. Carbohydr Res 185:131–138. https://doi.org/10.1016/0008-6215(89)84028-5
Article Google Scholar
Smetanajr K (1993) Cell biology of hydrogels. Biomaterials 14:1046–1050. https://doi.org/10.1016/0142-9612(93)90203-E
Article CAS Google Scholar
Smidsrød O, Haug A, Larsen B, von Hofsten B, Williams DH, Bunnenberg E et al (1966) The influence of pH on the rate of hydrolysis of acidic polysaccharides. Acta Chem Scand 20:1026–1034. https://doi.org/10.3891/acta.chem.scand.20-1026
Article Google Scholar
Smith KA (2011) Edward jenner and the small pox vaccine. Front Immunol 2:21. https://doi.org/10.3389/fimmu.2011.00021
Article Google Scholar
Sone T, Nagamori E, Ikeuchi T, Mizukami A, Takakura Y, Kajiyama S et al (2002) A novel gene delivery system in plants with calcium alginate micro-beads. J Biosci Bioeng 94:87–91. https://doi.org/10.1016/S1389-1723(02)80123-2
Article CAS Google Scholar
Stanford ECC (1883) On algin, a new substance obtained from some of the commoner species of marine algae. Chem News 47:254
Google Scholar
Steinle H, Ionescu T-M, Schenk S, Golombek S, Kunnakattu S-J, Özbek MT et al (2018) Incorporation of synthetic mRNA in injectable chitosan-alginate hybrid hydrogels for local and sustained expression of exogenous proteins in cells. Int J Mol Sci 19:1313. https://doi.org/10.3390/ijms19051313
Article CAS Google Scholar
Sultan S, Abdelhamid HN, Zou X, Mathew AP (2018) Cello MOF: nanocellulose enabled 3D printing of metal-organic frameworks. Adv Funct Mater 29:1805372. https://doi.org/10.1002/adfm.201805372
Article CAS Google Scholar
Sutirman ZA, Sanagi MM, Wan Aini WI (2021) Alginate-based adsorbents for removal of metal ions and radionuclides from aqueous solutions: a review. Int J Biol Macromol 174:216–228. https://doi.org/10.1016/j.ijbiomac.2021.01.150
Article CAS Google Scholar
Tahamtan A, Charostad J, Hoseini Shokouh SJ, Barati M (2017) An overview of history, evolution, and manufacturing of various generations of vaccines. J Arch Mil Med 5(3):e12315. https://doi.org/10.5812/jamm.12315
Article Google Scholar
Tamay DG, Dursun Usal T, Alagoz AS, Yucel D, Hasirci N, Hasirci V (2019) 3D and 4D printing of polymers for tissue engineering applications. Front Bioeng Biotechnol 7:164. https://doi.org/10.3389/fbioe.2019.00164
Article Google Scholar
Tan W-H, Takeuchi S (2007) Monodisperse alginate hydrogel microbeads for cell encapsulation. Adv Mater 19:2696–2701. https://doi.org/10.1002/adma.200700433
Article CAS Google Scholar
Teng K, An Q, Chen Y, Zhang Y, Zhao Y (2021) Recent development of alginate-based materials and their versatile functions in biomedicine, flexible electronics, and environmental uses. ACS Biomater Sci Eng 7:1302–1337. https://doi.org/10.1021/acsbiomaterials.1c00116
Article CAS Google Scholar
Venkatesan J, Bhatnagar I, Kim S-K (2014) Chitosan-alginate biocomposite containing fucoidan for bone tissue engineering. Mar Drugs 12:300–316. https://doi.org/10.3390/md12010300
Article CAS Google Scholar
Wang K, Kievit FM, Florczyk SJ, Stephen ZR, Zhang M (2015) 3D porous chitosan–alginate scaffolds as an in vitro model for evaluating nanoparticle-mediated tumor targeting and gene delivery to prostate cancer. Biomacromolecules 16:3362–3372. https://doi.org/10.1021/acs.biomac.5b01032
Article CAS Google Scholar
Wegman F, Bijenhof A, Schuijff L, Öner F, Dhert W, Alblas J (2011) Osteogenic differentiation as a result of BMP-2 plasmid DNA based gene therapy in vitro and in vivo. Eur Cells Mater 21:230–242. https://doi.org/10.22203/eCM.v021a18
Article CAS Google Scholar
Wegman F, Geuze RE, van der Helm YJ, Cumhur Öner F, Dhert WJA, Alblas J (2014) Gene delivery of bone morphogenetic protein-2 plasmid DNA promotes bone formation in a large animal model. J Tissue Eng Regen Med 8:763–770. https://doi.org/10.1002/term.1571
Article CAS Google Scholar
Xu M, Qin M, Cheng Y, Niu X, Kong J, Zhang X et al (2021) Alginate microgels as delivery vehicles for cell-based therapies in tissue engineering and regenerative medicine. Carbohydr Polym 266:118128. https://doi.org/10.1016/j.carbpol.2021.118128
Article CAS Google Scholar
Xu C, Qiao M, Huo X, Liao Z, Su J (2022) An oral microencapsulated vaccine loaded by sodium alginate effectively enhances protection against GCRV infection in grass carp (Ctenopharyngodon idella). Front Immunol 13:848958. https://doi.org/10.3389/fimmu.2022.848958
Article CAS Google Scholar
Yang J, Hu X, Xu J, Liu X, Yang L (2018) Single-step in situ acetylcholinesterase-mediated alginate hydrogelation for enzyme encapsulation in CE. Anal Chem 90:4071–4078. https://doi.org/10.1021/acs.analchem.7b05353
Article CAS Google Scholar
Yang R, Chen F, Guo J, Zhou D, Luan S (2020) Recent advances in polymeric biomaterials-based gene delivery for cartilage repair. Bioact Mater 5:990–1003. https://doi.org/10.1016/j.bioactmat.2020.06.004
Article Google Scholar
You J-O, Liu Y-C, Peng C-A (2006) Efficient gene transfection using chitosan? alginate core-shell nanoparticles. Int J Nanomedicine 1:173–180. https://doi.org/10.2147/nano.2006.1.2.173
Article CAS Google Scholar
Zhao D, Zhuo R-X, Cheng S-X (2012a) Alginate modified nanostructured calcium carbonate with enhanced delivery efficiency for gene and drug delivery. Mol Bio Syst 8:753–759. https://doi.org/10.1039/C1MB05337J
Article CAS Google Scholar
Zhao D, Liu C-J, Zhuo R-X, Cheng S-X (2012b) Alginate/CaCO₃ hybrid nanoparticles for efficient codelivery of antitumor gene and drug. Mol Pharm 9:2887–2893. https://doi.org/10.1021/mp3002123
Article CAS Google Scholar
Zohri M, Arefian E, Akbari Javar H, Gazori T, Aghaee-Bakhtiari SH, Taheri M et al (2021) Potential of chitosan/alginate nanoparticles as a non-viral vector for gene delivery: formulation and optimization using D-optimal design. Mater Sci Eng C 128:112262. https://doi.org/10.1016/j.msec.2021.112262
Article CAS Google Scholar

Download references

Author information

Authors and Affiliations

Advanced Multifunctional Materials Laboratory, Department of Chemistry, Faculty of Science, Assiut University, Assiut, Egypt
Hani Nasser Abdelhamid
Proteomics Laboratory for Clinical Research and Materials Science, Department of Chemistry, Assiut University, Assiut, Egypt
Hani Nasser Abdelhamid
Nanotechnology Research Centre (NTRC), The British University in Egypt, Cairo, Egypt
Hani Nasser Abdelhamid

Authors

Hani Nasser Abdelhamid
View author publications
You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hani Nasser Abdelhamid .

Editor information

Editors and Affiliations

Department of Health & Family Welfare, Directorate of Health Services, Kolkata, West Bengal, India
Sougata Jana
Department of Chemistry, Indira Gandhi National Tribal University, Amarkantak, Madhya Pradesh, India
Subrata Jana

Rights and permissions

Reprints and permissions

Copyright information

About this chapter

Cite this chapter

Abdelhamid, H.N. (2023). Alginate in Gene and Vaccine Delivery. In: Jana, S., Jana, S. (eds) Alginate Biomaterial. Springer, Singapore. https://doi.org/10.1007/978-981-19-6937-9_14

Download citation

DOI: https://doi.org/10.1007/978-981-19-6937-9_14
Published: 01 February 2023
Publisher Name: Springer, Singapore
Print ISBN: 978-981-19-6936-2
Online ISBN: 978-981-19-6937-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)

Publish with us

Policies and ethics