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Patterning alginate hydrogels using light-directed release of caged calcium in a microfluidic device

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Abstract

This paper describes a simple reversible hydrogel patterning method for 3D cell culture. Alginate gel is formed in select regions of a microfluidic device through light-triggered release of caged calcium. In the pre-gelled alginate solution, calcium is chelated by DM-nitrophen (DM-n) to prevent cross-linking of alginate. After sufficient UV exposure the caged calcium is released from DM-n causing alginate to cross-link. The effect of using different concentrations of calcium and chelating agents as well as the duration of UV exposure is described. Since the cross-linking is based on calcium concentration, the cross-linked alginate can easily be dissolved by EDTA. We also demonstrate application of this capability to patterned microscale 3D co-culture using endothelial cells and osteoblastic cells in a microchannel.

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Acknowledgement

This material is based on work supported by the U.S. Army Research Laboratory and the U.S. Army Research Office under Contract Grant DAAD19-03-1-0168 and the NIH (HG004653).

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Author notes

  1. Bor-han Chueh

    Present address: Department of Chemistry, Stanford University, Stanford, CA, 94305, USA

Authors and Affiliations

  1. Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA

    Bor-han Chueh, Ying Zheng, Yu-suke Torisawa, Amy Y. Hsiao & Shuichi Takayama

  2. Periodontics and Oral Medicine, University of Michigan, Ann Arbor, MI, 48109, USA

    Chunxi Ge & Renny Franceschi

  3. School of Engineering and Applied Science, Harvard University, Cambridge, MA, 02115, USA

    Susan Hsiong, Nathaniel Huebsch & David J. Mooney

  4. Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, 02139, USA

    Nathaniel Huebsch

  5. Macromolecular Science & Engineering program, University of Michigan, Ann Arbor, MI, 48109, USA

    Shuichi Takayama

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  1. Bor-han Chueh
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  2. Ying Zheng
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Correspondence to Shuichi Takayama.

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Chueh, Bh., Zheng, Y., Torisawa, Ys. et al. Patterning alginate hydrogels using light-directed release of caged calcium in a microfluidic device. Biomed Microdevices 12, 145–151 (2010). https://doi.org/10.1007/s10544-009-9369-6

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  • DOI: https://doi.org/10.1007/s10544-009-9369-6

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