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Cryopreservation of Testicular Stem Cells and Its Application in Veterinary Science

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Stem Cells in Veterinary Science

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Abstract

Testis-derived spermatogonial stem cells (SSCs) and its in vitro counterpart, male germline stem (GS) cells, can repopulate the empty seminiferous tubules of infertile males and, therefore, are rapidly emerging as newer biotechnological tools for the treatment of male infertility, posthumous reproduction, preservation of elite germplasm and animal transgenesis. It also has potential application in the preservation and restoration of fertility in males with diseases/treatments affecting spermatogenesis. Consequently, the cryopreservation of SSC and GS cells is becoming pivotal in assisted reproductive technology (ART) for male reproduction. Cryopreservation of testis-derived stem cells is particularly indispensable for fertility preservation in pre-pubertal males, who have not yet begun their sperm production. This chapter provides an overview of the indications, applications, and strategies of fertility preservation through the cryopreservation of testicular stem cells. Recent advances on freezing and vitrification techniques, assessment of cryopreservation-induced injuries, and methods of their amelioration are also discussed.

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Acknowledgments

This work was supported by grant from Indian Council of Medical Research (ICMR), Government of India.

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  1. Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha, India

    Tanushree Patra, Rakesh Bhaskar & Mukesh Kumar Gupta

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  1. Tanushree Patra
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Correspondence to Mukesh Kumar Gupta .

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  1. Department of Bioinformatics, College of Animal Biotechnology, Ludhiana, Punjab, India

    Ratan Kumar Choudhary

  2. Department of Microbial and Environmental Biotechnology, College of Animal Biotechnology, Ludhiana, Punjab, India

    Shanti Choudhary

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Patra, T., Bhaskar, R., Gupta, M.K. (2021). Cryopreservation of Testicular Stem Cells and Its Application in Veterinary Science. In: Choudhary, R.K., Choudhary, S. (eds) Stem Cells in Veterinary Science. Springer, Singapore. https://doi.org/10.1007/978-981-16-3464-2_9

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