A New Procedure for Estimation of Nitrovin and Carbadox Residues and Their Metabolites in Food of Animal Origin

Abstract

In order to establishe the length of the protective period for which the animals are given food without chemical growth stimulators before slaughter two methods are proposed permitting wide use in sanitary control: 1/ A highly sensitive method of pulse-polarography has been worked out for the estimation of widely used growth stimulator nitrovin residues/1.5-bis/5-nitro-2-furyl/1.4-pentadiene-3-on amidinohydrazone/and its metabolites. In the tissues of the experimental animals examined were found residues at a level of about 50 ppb. The limit of sensitivity of the procedure used is 10 ppb.

2 / Based upon double-electron reduction of the quinoxaline circle a simple and sufficiently sensitive Polarographic method was worked out for the estimation of carbadox residues/quinoxaline-1.4-N-dioxide-2-methylcarbazate/and its main metabolite/quinoxaline-2-carboxylic acid/. With the sensitivity of the method of 0.1 ppm excretion curves were designed and residue levels were determined in tissues of pigs fed up to 56 days of age by feed containing 20 – 50 ppm of carbadox.