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Polarization-Resolved SHG Microscopy for Biomedical Applications

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Optical Polarimetric Modalities for Biomedical Research

Part of the book series: Biological and Medical Physics, Biomedical Engineering ((BIOMEDICAL))

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Abstract

Polarization-resolved second harmonic generation (PSHG) microscopy is a powerful technique capable of differentiating biological tissues as well as determining ultrastructural parameters important to the biological function of the tissue. As such, PSHG microscopy is particularly useful in biomedical research. Here, innovative and developing PSHG microscopy techniques and their application in the field of biomedicine are described. The polarization-resolved SHG microscopy techniques include modulation of laser linear polarization, circular dichroism, double Stokes Mueller polarimetry, and detection of outgoing linear laser polarization. These techniques have been used to investigate collagenous tissues, muscle tissue, and much more. This chapter concludes with an outlook of the future of PSHG microscopy and briefly describes advances being performed to use this technique for in vivo functional tissue studies.

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Acknowledgements

This book chapter was supported by the Natural Science and Engineering Research Council of Canada (NSERC) Discovery Grants Program (RGPIN-2018-05444), Canada’s Research Support Fund, and Saint Mary’s University.

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  1. Department of Chemistry, Saint Mary’s University, Halifax, NS, Canada

    Richard Cisek, MacAulay Harvey, Elisha Bennett, Hwanhee Jeon & Danielle Tokarz

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Correspondence to Danielle Tokarz .

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  1. Department of Biophysics, Manipal Academy of Higher Education, Manipal, Karnataka, India

    Nirmal Mazumder

  2. Laboratory of Biophotonics, National Research Tomsk State University, Tomsk, Russia

    Yury V. Kistenev

  3. Bulgarian Academy of Sciences, Institute of Electronics, Sofia, Bulgaria

    Ekaterina Borisova

  4. Department of Cell and Molecular Biology, Manipal Academy of Higher Education, Manipal, Karnataka, India

    Shama Prasada K.

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Cisek, R., Harvey, M., Bennett, E., Jeon, H., Tokarz, D. (2023). Polarization-Resolved SHG Microscopy for Biomedical Applications. In: Mazumder, N., Kistenev, Y.V., Borisova, E., Prasada K., S. (eds) Optical Polarimetric Modalities for Biomedical Research. Biological and Medical Physics, Biomedical Engineering. Springer, Cham. https://doi.org/10.1007/978-3-031-31852-8_9

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