Abstract
Odor detection and discrimination by olfactory systems in vertebrates and invertebrates depend both on the selective expression of individual olfactory receptor genes in subpopulations of olfactory sensory neurons, and on the targeting of the encoded proteins to the exposed, ciliated endings of sensory dendrites. Techniques to visualize the expression and localization of olfactory receptor gene products in vivo have been essential to reveal the molecular logic of peripheral odor coding and to permit investigation of the developmental and cellular neurobiology of this sensory system. Here, we describe methods for detection of olfactory receptor transcripts and proteins in the antennal olfactory organ of the fruit fly, Drosophila melanogaster, an important genetic model organism. We include protocols both for antennal cryosections and whole-mount antennae. These methods can be adapted for detection of receptor expression in other olfactory and gustatory tissues in Drosophila, as well as in the chemosensory systems of other insects.
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References
Nei M, Niimura Y, Nozawa M (2008) The evolution of animal chemosensory receptor gene repertoires: roles of chance and necessity. Nat Rev Genet 9:951–963
Fuss SH, Ray A (2009) Mechanisms of odorant receptor gene choice in Drosophila and vertebrates. Mol Cell Neurosci 41:101–112
Mombaerts P (2004) Odorant receptor gene choice in olfactory sensory neurons: the one receptor-one neuron hypothesis revisited. Curr Opin Neurobiol 14:31–36
Hallem EA, Dahanukar A, Carlson JR (2006) Insect odor and taste receptors. Annu Rev Entomol 51:113–135
Touhara K, Vosshall LB (2009) Sensing odorants and pheromones with chemosensory receptors. Annu Rev Physiol 71:307–332
Spehr M, Munger SD (2009) Olfactory receptors: G protein-coupled receptors and beyond. J Neurochem 109:1570–1583
Hansson BS (2002) A bug’s smell/research into insect olfaction. Trends Neurosci 25: 270–274
Benton R (2007) Sensitivity and specificity in Drosophila pheromone perception. Trends Neurosci 30:512–519
Vosshall LB, Stocker RF (2007) Molecular architecture of smell and taste in Drosophila. Annu Rev Neurosci 30:505–533
Stocker RF (1994) The organization of the chemosensory system in Drosophila melanogaster: a review. Cell Tissue Res 275:3–26
Nakagawa T, Vosshall LB (2009) Controversy and consensus: noncanonical signaling Âmechanisms in the insect olfactory system. Curr Opin Neurobiol 19:284–292
Benton R, Vannice KS, Gomez-Diaz C, Vosshall LB (2009) Variant ionotropic glutamate receptors as chemosensory receptors in Drosophila. Cell 136:149–162
Croset V, Rytz R, Cummins SF, Budd A, Brawand D, Kaessmann H, Gibson TJ, Benton R (2010) Ancient protostome origin of chemosensory ionotropic glutamate receptors and the evolution of insect taste and olfaction. PLoS Genet 6:e1001064
Larsson MC, Domingos AI, Jones WD, Chiappe ME, Amrein H, Vosshall LB (2004) Or83b encodes a broadly expressed odorant receptor essential for Drosophila olfaction. Neuron 43:703–714
Benton R, Sachse S, Michnick SW, Vosshall LB (2006) Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. PLoS Biol 4:e20
Couto A, Alenius M, Dickson BJ (2005) Molecular, anatomical, and functional organization of the Drosophila olfactory system. Curr Biol 15:1535–1547
Fishilevich E, Vosshall LB (2005) Genetic and functional subdivision of the Drosophila antennal lobe. Curr Biol 15:1548–1553
Abuin L, Bargeton B, Ulbrich MH, Isacoff EY, Kellenberger S, Benton R (2011) Functional architecture of olfactory ionotropic glutamate receptors. Neuron 69:44–60
Dobritsa AA, Goes van Naters W van der, Warr CG, Steinbrecht RA, Carlson JR (2003) Integrating the molecular and cellular basis of odor coding in the Drosophila antenna. Neuron 37:827–841
Ai M, Min S, Grosjean Y, Leblanc C, Bell R, Benton R, Suh GS (2010) Acid sensing by the Drosophila olfactory system. Nature 468:691–695
Grosjean Y, Rytz R, Farine JP, Abuin L, Cortot J, Jefferis GS, Benton R (2011) An olfactory receptor for food-derived odours promotes male courtship in Drosophila. Nature 478: 236–240
Hallem EA, Carlson JR (2006) Coding of odors by a receptor repertoire. Cell 125: 143–160
Silbering AF, Rytz R, Grosjean Y, Abuin L, Ramdya P, Jefferis GS, Benton R (2011) Complementary function and integrated wiring of the evolutionarily distinct Drosophila olfactory subsystems. J Neurosci 31: 13357–13375
Ramdya P, Benton R (2010) Evolving olfactory systems on the fly. Trends Genet 26: 307–316
Hansson BS, Stensmyr MC (2011) Evolution of insect olfaction. Neuron 72:698–711
Acknowledgments
We thank Liliane Abuin, Vincent Croset, Jaime Reina, and Raphael Rytz for comments on this chapter. We acknowledge Elane Fishilevich and Kirsten Vannice (Vosshall Lab, Rockefeller University) and Jeanne de Lavallaz, who contributed to the development of these methods. Research in R.B.’s laboratory is supported by the University of Lausanne, a European Research Council Starting Independent Researcher Grant and the Swiss National Science Foundation.
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Saina, M., Benton, R. (2013). Visualizing Olfactory Receptor Expression and Localization in Drosophila . In: Crasto, C. (eds) Olfactory Receptors. Methods in Molecular Biology, vol 1003. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-377-0_16
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DOI: https://doi.org/10.1007/978-1-62703-377-0_16
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