Abstract
In the field of proteomics, numerous advanced technologies have evolved that aim to provide the molecular data necessary for an in-depth understanding of biological processes. Protein–protein interactions (PPI) are at the heart of cellular function and a milestone yet to be achieved is the mapping of a complete human interactome. Bioluminescence resonance energy transfer (BRET) has become a popular technique to investigate PPI. As BRET enables the detection of PPI in living cells, problems associated with in vitro biochemical assays can be circumvented, thus making BRET a powerful tool for monitoring interactions of virtually all kinds of protein species.
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Acknowledgments
Financial support from the Bavarian Genome Research Network (BayGene) and the LMUexcellent grant 42595-6 to A.C.M. is gratefully acknowledged.
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Gersting, S.W., Lotz-Havla, A.S., Muntau, A.C. (2012). Bioluminescence Resonance Energy Transfer: An Emerging Tool for the Detection of Protein–Protein Interaction in Living Cells. In: Kaufmann, M., Klinger, C. (eds) Functional Genomics. Methods in Molecular Biology, vol 815. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-424-7_19
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DOI: https://doi.org/10.1007/978-1-61779-424-7_19
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