Abstract
In this chapter we describe protocols for immunolabeling and FISH of pollen grains undergoing postmeiotic mitosis using Aegilops speltoides, Secale cereale, and Hordeum vulgare as models. Tissue sectioning of pollen overcomes the problem of the pollen grain wall impermeability that interferes with immunolocalization and in situ hybridization. The crucial element of the protocol is the generation and immobilization of tissue sections. Our method facilitates the investigation of the microspore cell divisions and pollen grain maturation.
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Acknowledgments
We thank the Deutsche Forschungsgemeinschaft DFG (Ho1779/26-1, 30-1) and the China Scholarship Council (CSC, 201606910015) for financial support.
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Wu, D.D., Ruban, A., Rutten, T., Zhou, Y.H., Houben, A. (2020). Analysis of Pollen Grains by Immunostaining and FISH in Triticeae Species. In: Pradillo, M., Heckmann, S. (eds) Plant Meiosis. Methods in Molecular Biology, vol 2061. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9818-0_24
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DOI: https://doi.org/10.1007/978-1-4939-9818-0_24
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