Abstract
Super-resolution microscopy enables the study of vaccinia architecture at subviral resolution with molecular specificity. Here, we outline how to use structured illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM) to detect fluorescently tagged or immunolabeled viral proteins on purified virions. Tens to hundreds of individual virions can be imaged in a single field of view providing data for single-particle averaging or quantitative analysis of viral protein spatial organization.
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Acknowledgments
R.G. is funded by the Engineering and Physical Sciences Research Council (EP/M506448/1). D.A. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 750673.
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Gray, R., Albrecht, D. (2019). Super-resolution Microscopy of Vaccinia Virus Particles. In: Mercer, J. (eds) Vaccinia Virus. Methods in Molecular Biology, vol 2023. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9593-6_16
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DOI: https://doi.org/10.1007/978-1-4939-9593-6_16
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