Abstract
Transcriptional activation by STAT5 is repressed by deacetylase inhibitors. Investigating the role of deacetylases (HDACs) in STAT5-mediated transcription implies the analysis of molecular events taking place at the chromatin level. We describe here two alternative methods of chromatin immunoprecipitation that allow the characterization of chromatin modifications ensuing STAT5 activation and its inhibition by deacetylase inhibitors, in particular changes in histone acetylation, in histone occupancy, and in the association/dissociation of transcription factors and other chromatin-associated factors.
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Acknowledgement
This work was supported by the Deutsche Forschungsgemeinschaft [RA 2010/2–1 to A.R.], the Deutsche Krebshilfe [109750 to A.R.], and institutional research funds [Foerderlinie C to A.R.; Frauenfoerderung, Bayerisches Programm zur Realisierung der Chancengleichheit fuer Frauen in Forschung und Lehre, to S.P.]. We thank Samy Unser for his permission to use part of an unpublished agarose gel picture (Fig. 1a).
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Pinz, S., Rascle, A. (2017). Assessing HDAC Function in the Regulation of Signal Transducer and Activator of Transcription 5 (STAT5) Activity Using Chromatin Immunoprecipitation (ChIP). In: Krämer, O. (eds) HDAC/HAT Function Assessment and Inhibitor Development. Methods in Molecular Biology, vol 1510. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6527-4_19
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DOI: https://doi.org/10.1007/978-1-4939-6527-4_19
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