Overview
- Editors:
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Michael Kyba
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Lillehei Heart Institute, University of Minnesota, Minneapolis, USA
- Includes cutting-edge methods and protocols
- Provides step-by-step details essential for reproducible results
- Contains key notes and implementation advice from the experts
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Table of contents (23 protocols)
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Injury Models
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- Jarrod A. Call, Dawn A. Lowe
Pages 3-18
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- Beth E. Pollot, Benjamin T. Corona
Pages 19-31
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- Gengyun Le, Dawn A. Lowe, Michael Kyba
Pages 33-41
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- Tyler J. Kirby, John J. McCarthy, Charlotte A. Peterson, Christopher S. Fry
Pages 43-52
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- Matthew T. Tierney, Alessandra Sacco
Pages 53-60
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- Glynnis A. Garry, Marie Lue Antony, Daniel J. Garry
Pages 61-71
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- Patrizia Pessina, Pura Muñoz-Cánoves
Pages 73-82
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Evaluating Progenitor Cells
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- Huascar P. Ortuste Quiroga, Katsumasa Goto, Peter S. Zammit
Pages 85-100
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- Pascal Stuelsatz, Zipora Yablonka-Reuveni
Pages 101-127
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- Mayank Verma, Bhavani SR Murkonda, Yoko Asakura, Atsushi Asakura
Pages 129-140
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- Thomas O. Vogler, Katherine E. Gadek, Adam B. Cadwallader, Tiffany L. Elston, Bradley B. Olwin
Pages 141-162
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- Robert W. Arpke, Michael Kyba
Pages 163-179
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- Andrew T. V. Ho, Helen M. Blau
Pages 181-189
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- Alessandro Magli, Tania Incitti, Rita C. R. Perlingeiro
Pages 191-208
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- Laura GarcÃa-Prat, Marta MartÃnez-Vicente, Pura Muñoz-Cánoves
Pages 223-240
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- Akiyoshi Uezumi, Takehiro Kasai, Kunihiro Tsuchida
Pages 241-253
About this book
This volume focuses
on the cell biology and physiology of skeletal muscle regeneration. This Book
is a collection of classic and cutting edge protocols optimized for mice, but
in most cases adaptable to rat or other mammalian models, that will allow an
investigator to develop and implement a research study on skeletal muscle
regeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing the
progenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury by
eccentric contraction; volumetric muscle loss; single myofiber isolation and
culture; satellite cell transplantation; muscle clearing for whole mount
immunostaining; luciferase tracking of muscle stem cells; mitochondrial
and mitophagy flux analysis; in vivo assessment of muscle contractility;
force measurements on single isolated myofibers; and analysis of aerobic
respiration in intact skeletal muscle tissue by microplate respirometry. Written in the highly
successful Methods in Molecular Biology series format,
chapters include introductions to each respective topic, lists of the necessary
materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and
practical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research in
skeletal muscle growth, damage, repair, degeneration, and regenerative therapy
in the mouse model system.
Editors and Affiliations
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Lillehei Heart Institute, University of Minnesota, Minneapolis, USA
Michael Kyba
About the editor
Michael Kyba, Ph.D.
Associate Professor and Lillehei Endowed Scholar, Lillehei Heart Institute NHH4.126, University of Minnesota, SE
Minneapolis, MN 55455, USA