Abstract
High-resolution melt (HRM) curve analysis is a PCR-based assay that identifies sequence alterations based on subtle variations in the melting curves of mutated versus wild-type DNA sequences. HRM analysis is a high-throughput, sensitive, and efficient alternative to Sanger sequencing and is used to assess for mutations in clinically important genes involved in cancer diagnosis. The technique involves PCR amplification of a target sequence in the presence of a fluorescent double-stranded DNA (dsDNA) binding dye, melting of the fluorescent amplicons, and subsequent interpretation of melt curve profiles.
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Mehrotra, M., Patel, K.P. (2016). High-Resolution Melt Curve Analysis in Cancer Mutation Screen. In: Luthra, R., Singh, R., Patel, K. (eds) Clinical Applications of PCR. Methods in Molecular Biology, vol 1392. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3360-0_7
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DOI: https://doi.org/10.1007/978-1-4939-3360-0_7
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3358-7
Online ISBN: 978-1-4939-3360-0
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