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Common Methods of Extraction and Determination of Phytomelatonin in Plants

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ROS Signaling in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2798))

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Abstract

The presence of melatonin in plants, called phytomelatonin, has gained great interest in recent years. The determination of phytomelatonin levels in plant extracts for both physiological and plant foodstuff studies requires sophisticated techniques due to the low endogenous levels of this indolic compound with hormonal nature. This chapter presents the most common and advanced techniques in the determination of phytomelatonin, with special emphasis on the techniques of extraction, cleaning, separation, detection, identification, and quantification. Multiple examples and recommendations are presented for a clear overview of the pros and cons of phytomelatonin determinations in plant tissues, seeds, and fruits, mainly.

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Acknowledgments

The research was funded by the Ministerio de Ciencia e Innovación (Spain), “Proyectos de I+D+,” Programas Estatales de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i y de I+D+i Orientada a los Retos de la Sociedad, del Plan Estatal de Investigación Científica y Técnica y de Innovación 2017–2020, Grant PID2020-113029RB-I00, funded by MCIN/AEI/ 10.13039/501100011033. More information can be found on Phytohormones and Plant Development Research Group web page at https://www.um.es/en/web/phytohormones (accessed October 22, 2023)a.

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Authors and Affiliations

  1. Phytohormones and Plant Development Laboratory, Department of Plant Biology (Plant Physiology), Faculty of Biology, University of Murcia, Murcia, Spain

    Antonio Cano, Josefa Hernández-Ruiz & Marino B. Arnao

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  1. Antonio Cano
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  2. Josefa Hernández-Ruiz
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  3. Marino B. Arnao
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Correspondence to Marino B. Arnao .

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  1. Spanish National Research Council, Estación Experimental del Zaidín, Granada, Granada, Spain

    Francisco J. Corpas

  2. Spanish National Research Council, Estación Experimental del Zaidín, Granada, Spain

    José M. Palma

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Cano, A., Hernández-Ruiz, J., Arnao, M.B. (2024). Common Methods of Extraction and Determination of Phytomelatonin in Plants. In: Corpas, F.J., Palma, J.M. (eds) ROS Signaling in Plants . Methods in Molecular Biology, vol 2798. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3826-2_11

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