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Cerebral Cortex Development

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Koh-ichi Nagata

    1. Institute for Developmental Research, Aichi Developmental Disability Center, Aichi, Japan

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2794)

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About this book

This volume explores the latest developments in the study of the cerebral cortex. The chapters in this book cover a wide array or established methods such as in situ hybridization of brain slices; two-photon FRET/FLIM imaging of cerebral neurons; BioID analysis of actin-binding proteins; generation of iPS cells using Sendai Virus vectors; in vivo whole-cell recording from the mouse brain; and locomotor assays in Drosphila larvae and adult flies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Cerebral Cortex Development: Methods and Protocols is a valuable resource that will help both expert and novice researchers further enhance their understanding of this important field.


Keywords

Table of contents (29 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Overview: Research on the Genetic Architecture of the Developing Cerebral Cortex in Norms and Diseases

    • Mariko Y. Momoi
    Pages 1-12

  3. In Situ Hybridization of Brain Slices

    • Tomohiro Kanayama, Hiroyuki Tomita, Akira Hara
    Pages 13-19

  4. Immunohistochemistry of Brain Tissues

    • Akira Hara, Toshiaki Taniguchi, Tomohiro Kanayama, Hiroyuki Tomita
    Pages 21-32

  5. Two-Photon FRET/FLIM Imaging of Cerebral Neurons

    • Thomas T. Luyben, Jayant Rai, Bingyue Zhou, Hang Li, Kenichi Okamoto
    Pages 33-43

  6. Pre- and Postembedding Immunoelectron Microscopy to Analyze the Central Nervous System

    • Fumino Fujiyama, Fuyuki Karube
    Pages 45-62

  7. Mouse Brain Tissue Preparation for Scanning Electron Microscopy

    • Kosuke Mori, Chihiro Takada, Hideshi Okada, Hiroyuki Tomita
    Pages 63-70

  8. Simple Method for the Preparation of Postsynaptic Density Fraction from Mouse Brain

    • Hidenori Ito, Rika Morishita, Koh-ichi Nagata
    Pages 71-78

  9. Reconstitution of Neuronal Motor Traffic on Septin-Associated Microtubules

    • Yani Suber, Elias T. Spiliotis
    Pages 79-94

  10. BioID Analysis of Actin-Binding Proteins

    • E. Emily Joo, Michael F. Olson
    Pages 95-104

  11. Proteomic Analyses of the Mouse Brain

    • Jing Li
    Pages 105-120

  12. Generation of iPSCs Using Sendai Virus Vectors

    • Hitomi Aoki
    Pages 121-140

  13. Directed Differentiation of Neurons from Human iPSCs for Modeling Neurological Disorders

    • Cheng Wang, Jonas Cerneckis, Yanhong Shi
    Pages 141-155

  14. Generating Homogeneous Brain Organoids from Human iPSCs

    • Xianwei Chen, Yanhong Shi
    Pages 157-167

  15. Primary Culture of Dissociated Neurons from the Embryonic Cerebral Cortex

    • Shiho Ito, Megumi Kawasaki, Takeshi Kawauchi
    Pages 169-175

  16. Immunocytochemistry of Primary Cultured Cerebral Cortical Neurons

    • Shiho Ito, Takeshi Kawauchi
    Pages 177-186

  17. Brain Sample Preparation After Performing in Utero Electroporation to Proliferating and Differentiated Cells in the Developing Mouse Neocortex

    • Minkyung Shin, Yoichi Kosodo
    Pages 187-200

  18. Live Imaging of Migrating Neurons and Glial Progenitors Visualized by in Utero Electroporation

    • Masashi Nishikawa, Koh-ichi Nagata, Hidenori Tabata
    Pages 201-209

  19. Ca2+ Imaging in Immature Cortical Neurons

    • Shin-ichiro Horigane, Sayaka Takemoto-Kimura
    Pages 211-219

  20. In Vitro Patch-Clamp

    • Toshihiro Nomura
    Pages 221-244
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Editors and Affiliations

  • Institute for Developmental Research, Aichi Developmental Disability Center, Aichi, Japan

    Koh-ichi Nagata

Bibliographic Information

  • Book Title: Cerebral Cortex Development

  • Book Subtitle: Methods and Protocols

  • Editors: Koh-ichi Nagata

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3810-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3809-5Published: 18 April 2024

  • Softcover ISBN: 978-1-0716-3812-5Due: 19 May 2024

  • eBook ISBN: 978-1-0716-3810-1Published: 17 April 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 355

  • Number of Illustrations: 24 b/w illustrations, 77 illustrations in colour

  • Topics: Neurosciences

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