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Two-Photon FRET/FLIM Imaging of Cerebral Neurons

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Cerebral Cortex Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2794))

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Abstract

Two-photon FRET (Förster resonance energy transfer) and FLIM (fluorescence lifetime imaging microscopy) enable the detection of FRET changes of fluorescence reporters in deep brain tissues, which provide a valuable approach for monitoring target molecular dynamics and functions. Here, we describe two-photon FRET and FLIM imaging techniques that allow us to visualize endogenous and optogenetically induced cAMP dynamics in living neurons with genetically engineered FRET-based cAMP reporters.

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Authors and Affiliations

  1. Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada

    Thomas T. Luyben, Jayant Rai, Bingyue Zhou, Hang Li & Kenichi Okamoto

  2. Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, ON, Canada

    Thomas T. Luyben, Jayant Rai, Bingyue Zhou & Kenichi Okamoto

Authors
  1. Thomas T. Luyben
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  2. Jayant Rai
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  3. Bingyue Zhou
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  4. Hang Li
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  5. Kenichi Okamoto
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Corresponding author

Correspondence to Kenichi Okamoto .

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Editors and Affiliations

  1. Institute for Developmental Research, Aichi Developmental Disability Center, Aichi, Japan

    Koh-ichi Nagata

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Luyben, T.T., Rai, J., Zhou, B., Li, H., Okamoto, K. (2024). Two-Photon FRET/FLIM Imaging of Cerebral Neurons. In: Nagata, Ki. (eds) Cerebral Cortex Development. Methods in Molecular Biology, vol 2794. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3810-1_4

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  • DOI: https://doi.org/10.1007/978-1-0716-3810-1_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3809-5

  • Online ISBN: 978-1-0716-3810-1

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