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Simultaneous Behavioral and Neuronal Imaging by Tracking Microscopy

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Zebrafish

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2707))

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Abstract

Tracking microscopy enables whole-brain cellular resolution imaging in freely swimming animals. This technique enables both structural and functional imaging without immobilizing the animal, and greatly expands the range of the behaviors accessible to neuroscientists. We use infrared imaging to track the target animal in a behavioral arena. Based on the predicted trajectory of the brain, we apply optimal control theory to a motorized stage system to cancel brain motion in three dimensions. We have combined this motion cancellation system with Differential Illumination Focal Filtering (DIFF), a form of structured illumination microscopy, which enables us to image the brain of a freely swimming larval zebrafish for over an hour. Here we describe the typical experimental procedure for data acquisition and processing using the tracking microscope.

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Author information

Author notes

  1. Drew N. Robson and Jennifer M. Li contributed equally to this work

Authors and Affiliations

  1. Max Planck Institute for Biological Cybernetics, Tuebingen, Germany

    Drew N. Robson & Jennifer M. Li

Authors
  1. Drew N. Robson
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  2. Jennifer M. Li
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Corresponding author

Correspondence to Drew N. Robson .

Editor information

Editors and Affiliations

  1. Department of Pediatrics Children’s Hospital Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

    James F. Amatruda

  2. Centre for Developmental Neurobiology, MRC Centre for NeuroDevelopmental Disorders, King’s College London, London, UK

    Corinne Houart

  3. Laboratory of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka, Japan

    Koichi Kawakami

  4. Duke Regeneration Center, Department of Cell Biology, Duke University Medical Center, Durham, NC, USA

    Kenneth D. Poss

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Robson, D.N., Li, J.M. (2024). Simultaneous Behavioral and Neuronal Imaging by Tracking Microscopy. In: Amatruda, J.F., Houart, C., Kawakami, K., Poss, K.D. (eds) Zebrafish. Methods in Molecular Biology, vol 2707. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3401-1_10

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  • DOI: https://doi.org/10.1007/978-1-0716-3401-1_10

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3400-4

  • Online ISBN: 978-1-0716-3401-1

  • eBook Packages: Springer Protocols

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