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Inflammation and Cancer

Methods and Protocols

  • Book
  • © 2023
  • Latest edition

Overview

Editors:
  1. Brendan J. Jenkins

    1. Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Clayton, Australia

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2691)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Experimental Model Systems

    1. Front Matter

      Pages 1-1
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    2. Identifying Adult Stomach Tissue Stem/Progenitor Cells Using the Iqgap3-2A-CreERT2 Mouse

      • Junichi Matsuo, Linda Shyue Huey Chuang, Jasmine Jie Lin Tong, Daisuke Douchi, Yoshiaki Ito
      Pages 3-17

    3. In Vitro and In Vivo Models for Metastatic Intestinal Tumors Using Genotype-Defined Organoids

      • Atsuya Morita, Mizuho Nakayama, Hiroko Oshima, Masanobu Oshima
      Pages 19-30

    4. On Target: An Intrapulmonary Transplantation Method for Modelling Lung Tumor Development in its Native Microenvironment

      • Jackson A. McDonald, Leanne Scott, Jessica Van Zuylekom, Steven Holloway, Benjamin J. Blyth, Kate D. Sutherland
      Pages 31-41

    5. Endoscopic Ultrasound-Guided Fine-Needle Biopsies to Generate Preclinical Disease Models to Study Inflammation in Pancreatic Ductal Adenocarcinoma

      • Joanne Lundy, Daniel Croagh
      Pages 43-54

    6. Modeling Intestinal Carcinogenesis Using In Vitro Organoid Cultures

      • Wing Hei Chan, Diana Micati, Rebekah M. Engel, Genevieve Kerr, Reyhan Akhtar, Thierry Jardé et al.
      Pages 55-69

    7. An In Vitro Model for Assessing Acute Lung Injury During Pancreatitis Development Using Primary Mouse Cell Co-cultures

      • Mohamed I. Saad, Brendan J. Jenkins
      Pages 71-80

    8. Tracking the Host Response to Infection in Peritoneal Models of Acute Resolving Inflammation

      • David Millrine, Christopher M. Rice, Javier U. Fernandez, Simon A. Jones
      Pages 81-95

    9. Assessing Lung Inflammation and Pathology in Preclinical Models of Chronic Obstructive Pulmonary Disease

      • Ross Vlahos, Hao Wang, Steven Bozinovski
      Pages 97-109

    10. Preclinical Mouse Model of Silicosis

      • Maggie Lam, Ashley Mansell, Michelle D. Tate
      Pages 111-120

  3. Experimental Techniques

    1. Front Matter

      Pages 121-121
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    2. Tracking Cardiovascular Comorbidity in Models of Chronic Inflammatory Disease

      • Aisling S. Morrin, Simon Eastham, Anwen S. Williams, Gareth W. Jones
      Pages 123-137

    3. Live Imaging of Pyroptosis in Primary Murine Macrophages

      • Caroline L. Holley, Kate Schroder
      Pages 139-153

    4. A Streamlined Method for Detecting Inflammasome-Induced ASC Oligomerization Using Chemical Crosslinking

      • Sebastian A. Hughes, James E. Vince
      Pages 155-164

    5. DNA Methylation Analysis

      • Naoko Hattori, Yu-Yu Liu, Toshikazu Ushijima
      Pages 165-183

    6. Flow Cytometry Identification of Cell Compartments in the Murine Brain

      • Joel J. D. Moffet, Zachery Moore, Shannon J. Oliver, Tahnee Towers, Misty R. Jenkins, Saskia Freytag et al.
      Pages 185-198

    7. Expression and Purification of Inflammasome Sensor NOD-Like Receptor Protein-1 Using the Baculovirus-Insect Cell Expression System

      • Hariharan Sivaraman, Wilson Wong
      Pages 199-206

    8. Dissecting Interleukin-6 Classic and Trans-signaling in Inflammation and Cancer

      • Christoph Garbers, Stefan Rose-John
      Pages 207-224

    9. Selecting Therapeutic Antisense Oligonucleotides with Gene Targeting and TLR8 Potentiating Bifunctionality

      • Sunil Sapkota, Michael P. Gantier
      Pages 225-234
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Keywords

About this book

This second edition volume expands on the previous edition with an update on the broad spectrum of research models, techniques, and protocols used in laboratories by basic and clinical researchers. The chapters in this book are divided into two parts. Part One discusses the latest findings on the development and characterization of representative research models for chronic immune-based diseases and inflammation-associated cancers. Part Two covers biochemical, molecular, and cellular biological techniques that are commonly used to dissect the molecular mechanisms and cellular processes that drive the pathogenesis of certain disease states. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and comprehensive, Inflammation and Cancer: Methods and Protocols, Second Edition is a valuable resource for those with a diverse range of laboratory-based experience, ranging from novice undergraduate students to established basic or clinical researchers who wish to diversify their existing portfolio of practical knowledge in the field. 


Editors and Affiliations

  • Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Clayton, Australia

    Brendan J. Jenkins

Bibliographic Information

  • Book Title: Inflammation and Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Brendan J. Jenkins

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3331-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3330-4Published: 26 June 2023

  • Softcover ISBN: 978-1-0716-3333-5Due: 26 July 2023

  • eBook ISBN: 978-1-0716-3331-1Published: 24 June 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVI, 373

  • Number of Illustrations: 11 b/w illustrations, 61 illustrations in colour

  • Topics: Cancer Research

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