Abstract
The growth and development of plants depends on diversified gene expression in different cell types. Compared to traditional bulk RNA sequencing, droplet-based single-cell RNA sequencing (scRNA-seq) allows for transcriptome profiling of individual cells within heterogeneous tissues. scRNA-seq provides a high-resolution atlas of cellular characterization and vastly improves our understandings of the interactions between individual cells and the microenvironment. However, the difficulty in protoplast isolation has limited the application of single-cell sequencing technology in plant research. Here we describe a high-efficiency protoplast isolation protocol for scRNA-seq.
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Acknowledgments
This work was supported by the grants 2019YFA0903902Â and 2022YFE0101100 from National Key R&D Program of China (NKP), the grant 32270345Â from National Natural Science Foundation of China (NSFC), the grant 110202001021 (JY-04) from Bureau of National Tobacco, and the Fundamental Research Funds for the Central Universities to YW.
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Ren, S., Wang, Y. (2023). Protoplast Isolation for Plant Single-Cell RNA-seq. In: Riechmann, J.L., Ferrándiz, C. (eds) Flower Development . Methods in Molecular Biology, vol 2686. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3299-4_14
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DOI: https://doi.org/10.1007/978-1-0716-3299-4_14
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