Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2628)
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About this book
This third volume provides comprehensive protocols on pre-analytical, analytical, plasma, and serum proteomics. New and updated chapters are divided into nine sections, detailing blood processing and handling strategies, discovery- and targeted-based mass spectrometry, including workflows to aid in discovery and targeted data analysis, in addition to software and bioinformatics for the plasma proteome. This edition further integrates emerging areas in the development of technologies for plasma proteomics and assay platforms in biomarker discovery and translational proteomics, enrichment and detection strategies to understand the plasma proteome, and peptide, lipid and metabolite targeted assays. We also detail the emerging analysis of extracellular vesicles isolated from plasma.
Written in the format of the highly successful Methods in Molecular Biology series, each of the 33 chapters includes an introduction to the topic, lists necessary materials and methods, includes hints and tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Serum/Plasma Proteomics: Methods and Protocols, Third Edition aims to be comprehensive guide for researchers.
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Keywords
Table of contents (33 protocols)
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Blood Processing and Handling Strategies
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Discovery-Based Mass Spectrometry
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Developments in Technologies for Plasma Proteomics
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Enrichment & Detection Strategies
Editors and Affiliations
About the editors
Bibliographic Information
Book Title: Serum/Plasma Proteomics
Book Subtitle: Methods and Protocols
Editors: David W. Greening, Richard J. Simpson
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-2978-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023
Hardcover ISBN: 978-1-0716-2977-2Published: 14 February 2023
Softcover ISBN: 978-1-0716-2980-2Published: 14 February 2024
eBook ISBN: 978-1-0716-2978-9Published: 13 February 2023
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XVIII, 596
Number of Illustrations: 10 b/w illustrations, 50 illustrations in colour
Topics: Chemistry/Food Science, general, Bioinformatics, Mass Spectrometry