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Somatic Embryogenesis from Immature Olive Zygotic Embryos

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Somatic Embryogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2527))

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Abstract

The effects of plant growth regulators (PGRs) and the explant type on the embryogenesis and plant regeneration of olive (Olea europaea L. ssp europaea var. sativa) cv. “Chetoui” were studied using immature zygotic embryos. Embryogenic callus induction was achieved on OMc medium supplemented with different concentrations of BAP and NAA at low levels. Immature zygotic embryos as juvenile tissues are competent for somatic embryogenesis independently of PGRs supplementation. Repetitive somatic embryogenesis was obtained on PGRs-free media or at low concentrations in the dark.

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Acknowledgments

The authors gratefully acknowledge the support of Dr. N. Elloumi and Prof. M. Msallem and assistance of the technical staff of the Laboratory of Biotechnology and Plant Physiology in the National Agricultural Research Institute of Tunisia and the Olive Institute of Tunisia. In memory, Dr. Zina BELFELAH, former Senior Researcher at the Laboratory of Biotechnology and Plant Physiology in the National Agricultural Research Institute of Tunisia.

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Authors and Affiliations

  1. Tunis El Manar University, Tunis, Tunisia

    El Bahri Trabelsi

  2. National Agricultural Research Institute of Tunisia Laboratory of Horticulture, University of Carthage, Tunis, Tunisia

    Emna Jedidi

Authors
  1. El Bahri Trabelsi
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  2. Emna Jedidi
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Correspondence to El Bahri Trabelsi .

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Editors and Affiliations

  1. Laboratorio de Micropropagación Vegetal, Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana, Veracruz, Mexico

    Marco A. Ramírez-Mosqueda

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Trabelsi, E.B., Jedidi, E. (2022). Somatic Embryogenesis from Immature Olive Zygotic Embryos. In: Ramírez-Mosqueda, M.A. (eds) Somatic Embryogenesis. Methods in Molecular Biology, vol 2527. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2485-2_11

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  • DOI: https://doi.org/10.1007/978-1-0716-2485-2_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2484-5

  • Online ISBN: 978-1-0716-2485-2

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