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Embryonic Stem Cell Protocols

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  • © 2022
  • Latest edition

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Editors:
  1. Kursad Turksen

    1. Ottawa, ON, Canada

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2520)

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About this book

This fully updated volume explores improved and extended protocols for embryonic stem cell (ESC) research, provided with the most direct and informative methodologies currently available. The book examines how these models for cell lineage and differentiation studies have continued to mature into a critical research workhorse. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Embryonic Stem Cell Protocols, Fourth Edition serves as an ideal resource for researching mining the depths of the ESC field.

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Dissecting Molecular Phenotypes Through FACS-Based Pooled CRISPR Screens

    • Oriana Genolet, Liat Ravid Lustig, Edda G. Schulz
    Pages 1-24

  3. Feeder-Free Human Embryonic Stem Cell Culture Under Defined Culture Conditions

    • Taha Bartu Hayal, AyÅŸegül DoÄŸan
    Pages 25-35

  4. Rat-Induced Pluripotent Stem Cells-Derived Cardiac Myocytes in a Cell Culture Dish

    • Fatima Bianca A. Dessouki, Pawan K. Singal, Dinender K. Singla
    Pages 37-51

  5. Transient Induction and Characterization of Mouse Epiblast-Like Cells from Mouse Embryonic Stem Cells

    • Federico Pecori, Shoko Nishihara
    Pages 53-58

  6. High Content Image Analysis of Spatiotemporal Proliferation and Differentiation Patterns in 3D Embryoid Body Differentiation Model

    • Olga Gordeeva
    Pages 59-79

  7. Derivation of Multipotent Neural Progenitors from Human Embryonic Stem Cells for Cell Therapy and Biomedical Applications

    • Loriana Vitillo, Ludovic Vallier
    Pages 81-100

  8. Feeder-Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol

    • Hatice Burcu ÅžiÅŸli, Selinay Åženkal, Derya SaÄŸraç, Taha Bartu Hayal, AyÅŸegül DoÄŸan
    Pages 101-115

  9. ChIP-qPCR for Polycomb Group Proteins During Neuronal Differentiation of Human Pluripotent Stem Cells

    • Divya Desai, Prasad Pethe
    Pages 117-133

  10. Directed Differentiation of Human Pluripotent Stem Cells into Inner Ear Organoids

    • Yoshitomo Ueda, Stephen T. Moore, Eri Hashino
    Pages 135-150

  11. Glycolytic Profiling of Mouse Embryonic Stem Cells (mESCs)

    • Bibiana Correia, Maria Inês Sousa, João Ramalho-Santos
    Pages 151-159

  12. Replating Protocol for Human Induced Pluripotent Stem Cell–Derived Cardiomyocytes

    • Arzuhan Koc, Esra Cagavi
    Pages 161-170

  13. Hematopoietic Cell Isolation by Antibody-Free Flow Cytometry in the Zebrafish Embryo

    • Katsuhiro Konno, Jingjing Kobayashi-Sun, Fumio Arai, Isao Kobayashi, Daisuke Sugiyama
    Pages 171-180

  14. Human Trophectoderm Spheroid Derived from Human Embryonic Stem Cells

    • Wen Huang, Sze Wan Fong, William Shu Biu Yeung, Yin Lau Lee
    Pages 181-187

  15. Embryoid Bodies–Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions

    • Luciana Isaja, Sofía Luján Ferriol-Laffouillere, Sofía Mucci, María Soledad Rodríguez-Varela, Leonardo Romorini
    Pages 189-198

  16. A Simple, Rapid, and Cost-Effective Method for Loss-of-Function Assays in Pluripotent Cells

    • Helena Covelo-Molares, Yara Souto, Miguel Fidalgo, Diana Guallar
    Pages 199-213

  17. Embryoid Body Formation from Mouse and Human Pluripotent Stem Cells for Transplantation to Study Brain Microenvironment and Cellular Differentiation

    • Magdalena Guerra-Crespo, Omar Collazo-Navarrete, Rodrigo Ramos-Acevedo, Carmen Alejandra Morato-Torres, Birgitt Schüle
    Pages 215-232

  18. A Modified SMART-Seq Method for Single-Cell Transcriptomic Analysis of Embryoid Body Differentiation

    • Jianqun Zheng, Ying Ye, Qiushi Xu, Wei Xu, Wensheng Zhang, Xi Chen
    Pages 233-259

  19. Osteogenic Differentiation from Mouse Embryonic Stem Cells

    • Zahra Alvandi, Michal Opas
    Pages 261-264

  20. Murine Embryonic Stem Cell Culture, Self-Renewal, and Differentiation

    • Manar Elkenani, Belal A. Mohamed
    Pages 265-273
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Editors and Affiliations

  • Ottawa, ON, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Embryonic Stem Cell Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2437-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2436-4Published: 26 July 2022

  • Softcover ISBN: 978-1-0716-2439-5Published: 26 July 2023

  • eBook ISBN: 978-1-0716-2437-1Published: 25 July 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 4

  • Number of Pages: XIII, 363

  • Number of Illustrations: 1 b/w illustrations

  • Topics: Developmental Biology, Stem Cells, Cell Biology

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