Abstract
Mouse embryonic stem cells (mESCs) and mouse epiblast-like cells (mEpiLCs) recapitulate in vitro the epiblast first cell lineage decision, providing a powerful tool to investigate the mechanisms underlying the pluripotent state transition. Here, we describe a defined and robust protocol to transiently induce mEpiLCs from mESCs, together with a concise overview for their unbiased characterization for subsequent downstream applications.
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Acknowledgments
This work was supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number JP18K06139 (to S.N.) and Japan Science and Technology Agency (JST)-Mirai Program Grant Number JPMJMI18GB (to S.N.). F.P. was supported by Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) Scholarship (Top Global University Project).
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Pecori, F., Nishihara, S. (2021). Transient Induction and Characterization of Mouse Epiblast-Like Cells from Mouse Embryonic Stem Cells. In: Turksen, K. (eds) Embryonic Stem Cell Protocols . Methods in Molecular Biology, vol 2520. Humana, New York, NY. https://doi.org/10.1007/7651_2021_403
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DOI: https://doi.org/10.1007/7651_2021_403
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2436-4
Online ISBN: 978-1-0716-2437-1
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