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Protocols for In Vivo Doubled Haploid (DH) Technology in Maize Breeding: From Haploid Inducer Development to Haploid Genome Doubling

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Plant Gametogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2484))

Abstract

Doubled haploid (DH) technology reduces the time required to obtain homozygous genotypes and accelerates plant breeding among other advantages. It is established in major crop species such as wheat, barley, maize, and canola. DH lines can be produced by both in vitro and in vivo methods and the latter is focused here. The major steps involved in in vivo DH technology are haploid induction, haploid selection/identification, and haploid genome doubling. Herein, we elaborate on the various steps of DH technology in maize breeding from haploid induction to haploid genome doubling to produce DH lines. Detailed protocols on the following topics are discussed: in vivo haploid inducer line development, haploid selection using seed and root color markers and automated seed sorting based on embryo oil content using QSorter, artificial genome doubling, and the identification of genotypes with spontaneous haploid genome doubling (SHGD) ability.

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Acknowledgments

This work was supported by the Foundation for Food & Agriculture Research under award number CA19-SS-0000000128. The content of this book chapter is solely the responsibility of the authors and does not necessarily represent the official views of the Foundation for Food & Agriculture Research.

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Author notes

  1. Siddique I. Aboobucker and Talukder Z. Jubery have contributed equally to this work.

Authors and Affiliations

  1. Department of Agronomy, Iowa State University, Ames, IA, USA

    Siddique I. Aboobucker, Ursula K. Frei, Yu-Ru Chen, Tyler Foster & Thomas Lübberstedt

  2. Department of Mechanical Engineering, Iowa State University, Ames, IA, USA

    Talukder Z. Jubery & Baskar Ganapathysubramanian

Authors
  1. Siddique I. Aboobucker
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  2. Talukder Z. Jubery
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  3. Ursula K. Frei
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  4. Yu-Ru Chen
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  5. Tyler Foster
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  6. Baskar Ganapathysubramanian
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  7. Thomas Lübberstedt
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Corresponding author

Correspondence to Siddique I. Aboobucker .

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Editors and Affiliations

  1. Department of Plant Sciences, University of Cambridge, Cambridge, UK

    Christophe Lambing

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Aboobucker, S.I. et al. (2022). Protocols for In Vivo Doubled Haploid (DH) Technology in Maize Breeding: From Haploid Inducer Development to Haploid Genome Doubling. In: Lambing, C. (eds) Plant Gametogenesis. Methods in Molecular Biology, vol 2484. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2253-7_16

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  • DOI: https://doi.org/10.1007/978-1-0716-2253-7_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2252-0

  • Online ISBN: 978-1-0716-2253-7

  • eBook Packages: Springer Protocols

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