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Multiresidue Pesticide Analysis in Okra (Ladyfinger) Using Gas Chromatography Tandem Mass Spectrometry (GC-MS/MS)

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Pesticide Toxicology

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

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Abstract

The presence of pesticide residues in food and vegetables is a growing concern for consumers. In order to monitor these residues reliably, a selective and sensitive, multi-residue system has been developed and validated in okra (ladyfinger) by gas chromatography-tandem mass spectrometry (GC-MS/MS). The sample preparation procedure involves the extraction of the sample using the QuEChERS procedure with ethyl acetate, and the cleaning process involves the use of primary secondary amine (PSA) and the processing of two multiple reaction monitoring (MRM) transformations for each analyte by GC-MS/MS in electron impact (EI) mode. Precision and accuracy were tested by recovery studies. The process provides a cheaper and better alternative to the current multi-residue extraction techniques in the okra samples.

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Authors and Affiliations

  1. University of Agricultural Sciences, Dharwad, Karnataka, India

    Mahadev C. Khetagoudar & Mahadev B. Chetti

  2. Department of Applied Chemistry, School of Applied Natural Science, Adama Science and Technology University, Adama, Ethiopia

    Avvaru Praveen Kumar

  3. Department of Chemistry, Karnatak University, Dharwad, Karnataka, India

    Dinesh C. Bilehal

Authors
  1. Mahadev C. Khetagoudar
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  2. Mahadev B. Chetti
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  3. Avvaru Praveen Kumar
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  4. Dinesh C. Bilehal
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Editor information

Editors and Affiliations

  1. Laboratory of Drug-Toxicology, Universidade da Beira Interior, Covilhã, Portugal

    Eugenia Gallardo

  2. National Institute of Legal Medicine and Forensic Sciences- South Branch, Lisbon, Portugal

    Mário Barroso

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Khetagoudar, M.C., Chetti, M.B., Kumar, A.P., Bilehal, D.C. (2022). Multiresidue Pesticide Analysis in Okra (Ladyfinger) Using Gas Chromatography Tandem Mass Spectrometry (GC-MS/MS). In: Gallardo, E., Barroso, M. (eds) Pesticide Toxicology. Methods in Pharmacology and Toxicology. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1928-5_3

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  • DOI: https://doi.org/10.1007/978-1-0716-1928-5_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1927-8

  • Online ISBN: 978-1-0716-1928-5

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