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Clostridium spp. Toxins: A Practical Guide for Expression and Characterization in Escherichia coli

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Vaccine Design

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2411))

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Abstract

Farm animals are frequently affected by a group of diseases with a rapid clinical course, caused by Clostridium spp. and immunization is essential to provide protection. However, the current manufacturing platform for these vaccines has disadvantages and the main alternative is the use of an expression system that uses Escherichia coli to obtain recombinant vaccine antigens. In this chapter we describe procedures for cloning, expression and characterization of recombinant toxins from Clostridium spp. produced in E. coli for veterinary vaccine applications.

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References

  1. Uzal FA, Songer JG, Prescott JF, Popoff MR (2016) Clostridial diseases of animals. Wiley, Iowa

    Book  Google Scholar 

  2. Moreira GMSG et al (2016) Immunogenicity of a trivalent recombinant vaccine against clostridium perfringens alpha, beta, and epsilon toxins in farm ruminants. Sci Rep 6:1–9. https://doi.org/10.1038/srep22816

    Article  CAS  Google Scholar 

  3. Ferreira MRA et al (2016) Recombinant alpha, beta, and epsilon toxins of clostridium perfringens: production strategies and applications as veterinary vaccines. Toxins (Basel) 8(11):340. https://doi.org/10.3390/toxins8110340

    Article  CAS  Google Scholar 

  4. Zaragoza NE, Orellana CA, Moonen GA, Moutafis G, Marcellin E (2019) Vaccine production to protect animals against pathogenic clostridia. Toxins 11(9):525. https://doi.org/10.3390/toxins11090525

    Article  CAS  PubMed Central  Google Scholar 

  5. Moreira GMSG et al (2014) Production of recombinant botulism antigens: a review of expression systems. Anaerobe 28:130–136. https://doi.org/10.1016/j.anaerobe.2014.06.003

    Article  CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil

    Mariliana Luiza Ferreira Alves, Marcos Roberto Alves Ferreira, Rafael Amaral Donassolo, Clóvis Moreira Jr., Rafael Rodrigues Rodrigues & Fabricio Rochedo Conceição

  2. Institut fϋr Biochemie, Biotechnologie und Bioinformatik, Abteilung Biotechnologie, Technische Universität Braunschweig, Braunschweig, Germany

    Gustavo Marçal Schmidt Garcia Moreira

Authors
  1. Mariliana Luiza Ferreira Alves
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  2. Gustavo Marçal Schmidt Garcia Moreira
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  3. Marcos Roberto Alves Ferreira
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  4. Rafael Amaral Donassolo
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  5. Clóvis Moreira Jr.
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  6. Rafael Rodrigues Rodrigues
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  7. Fabricio Rochedo Conceição
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Corresponding author

Correspondence to Fabricio Rochedo Conceição .

Editor information

Editors and Affiliations

  1. Lankenau Institute for Medical Research, Wynnewood, PA, USA

    Sunil Thomas

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Alves, M.L.F. et al. (2022). Clostridium spp. Toxins: A Practical Guide for Expression and Characterization in Escherichia coli. In: Thomas, S. (eds) Vaccine Design. Methods in Molecular Biology, vol 2411. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1888-2_7

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  • DOI: https://doi.org/10.1007/978-1-0716-1888-2_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1887-5

  • Online ISBN: 978-1-0716-1888-2

  • eBook Packages: Springer Protocols

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