Abstract
The availability of protocols for virus-induced gene silencing (VIGS) in rice has opened up an important channel for the elucidation of gene functions in this important crop plant. Here, we present an updated protocol of a VIGS system based on Rice tungro bacilliform virus (RTBV) for gene silencing in rice. We present complete updated protocols for VIGS in rice, compare the system with other existing ones for monocots, identify some of the challenges faced by this system and discuss ways in which the vector could be improved for better silencing efficiency.
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Acknowledgments
GK acknowledges CSIR-Research Associateship from Council of Scientific and Industrial Research, New Delhi and KK acknowledges Research Fellowship from University Grants Commission, New Delhi for this work. The work in ID lab is funded by the J. C. Bose Fellowship awarded by the Science and Engineering Research Board, Department of Science and Technology, Government of India. The Departmental infrastructural facilities funded by the FIST programme of Department of Science and Technology, Government of India are also gratefully acknowledged.
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Kumar, G., Kumari, K., Dasgupta, I. (2022). RTBV-Based VIGS Vector for Functional Genomics in Rice: Methodology, Advances, Challenges, and Future Implications. In: Mysore, K.S., Senthil-Kumar, M. (eds) Plant Gene Silencing. Methods in Molecular Biology, vol 2408. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1875-2_8
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DOI: https://doi.org/10.1007/978-1-0716-1875-2_8
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Online ISBN: 978-1-0716-1875-2
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