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Heterologous Protein Production in Lactobacillus (plantarum) Using pSIP Vectors

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Insoluble Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2406))

Abstract

While lactobacilli are not generally regarded as efficient cell factories for heterologous proteins, these food-grade Gram-positive bacteria are attractive as expression hosts for medicinal proteins. Furthermore, tools have been developed not only to secrete the protein of interest, but also to anchor the protein to the cell membrane or the cell wall. Research efforts aimed at the production and surface display of complex vaccine proteins have shown that lactobacilli are capable of producing heterologous proteins that are otherwise difficult to produce in soluble form. Many recent studies on expressing a wide variety of proteins in lactobacilli have employed the pSIP vector system, which offers a wide range of possibilities for inducible expression, including various options for secretion and surface anchoring. The modular nature of the pSIP vectors allows for rapid screening of multiple expression strategies. This chapter describes the pSIP vector system and how it can be used to accomplish protein expression in lactobacilli.

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Acknowledgments

We thank previous lab members, as well as Dietmar Haltrich and Thu-Ha Nguyen at the University of Natural Resources and Life Sciences (BOKU) in Vienna, for their contributions to our work, which was supported by the Research Council of Norway, most recently by grants 196363 and 234502 awarded by the Globvac program.

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Authors and Affiliations

  1. Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Ås, Norway

    Geir Mathiesen & Vincent G. H. Eijsink

  2. Nofima AS, Norwegian Institute of Food, Fisheries and Aquaculture Research, Ås, Norway

    Lars Axelsson

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  1. Geir Mathiesen
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  2. Lars Axelsson
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  3. Vincent G. H. Eijsink
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Correspondence to Vincent G. H. Eijsink .

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Editors and Affiliations

  1. Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Caldes de Montbui, Spain

    Elena Garcia Fruitós

  2. Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Caldes de Montbui, Spain

    Anna Arís Giralt

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Mathiesen, G., Axelsson, L., Eijsink, V.G.H. (2022). Heterologous Protein Production in Lactobacillus (plantarum) Using pSIP Vectors. In: Garcia Fruitós, E., Arís Giralt, A. (eds) Insoluble Proteins. Methods in Molecular Biology, vol 2406. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1859-2_12

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  • DOI: https://doi.org/10.1007/978-1-0716-1859-2_12

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