Abstract
3′-Phosphoadenosine 5′-phosphosulfate transporters (PAPSTs) play an important role in transporting 3′-phosphoadenosine 5′-phosphosulfate (PAPS), the universal sulfuryl donor for sulfation, from the cytosol into the lumen of the Golgi apparatus. Here, we describe three methods for the analysis of PAPST; a transporter activity assay with yeast or mammalian cell fraction, real-time reverse transcription polymerase chain reaction on tissue samples, and immunohistochemistry on brain sections.
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Acknowledgments
Our research was partially supported by 476 JST-Mirai Program Grant Number JPMJMI18GB (to S.N.), Japan.
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Egawa, H., Nishihara, S. (2022). Analysis of 3′-Phosphoadenosine 5′-Phosphosulfate Transporters: Transporter Activity Assay, Real-Time Reverse Transcription Polymerase Chain Reaction, and Immunohistochemistry. In: Balagurunathan, K., Nakato, H., Desai, U., Saijoh, Y. (eds) Glycosaminoglycans. Methods in Molecular Biology, vol 2303. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1398-6_51
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DOI: https://doi.org/10.1007/978-1-0716-1398-6_51
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