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Glycosaminoglycans

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Kuberan Balagurunathan

    1. Departments of Biology, Bioengineering, and Medicinal Chemistry, University of Utah, Salt Lake City, USA

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  2. Hiroshi Nakato

    1. Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, USA

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  3. Umesh Desai

    1. Department of Medicinal Chemistry and Institute for Structural Biology, Drug Discovery and Development, Virginia Commonwealth University, Richmond, USA

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  4. Yukio Saijoh

    1. Department of Neurobiology & Department of Nutrition & Integrative Physiology, University of Utah, Salt Lake City, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2303)

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Table of contents (60 protocols)

  1. Front Matter

    Pages i-xxi
    Download chapter PDF

  2. GAG Structures

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. One-Pot Enzymatic Synthesis of Heparin from N-Sulfoheparosan

      • Li Fu, Robert J. Linhardt
      Pages 3-11

    3. Solution NMR Spectroscopy for Characterizing Protein–Glycosaminoglycan Interactions

      • Prem Raj B. Joseph, Krishna Mohan Sepuru, Krishna Mohan Poluri, Krishna Rajarathnam
      Pages 13-23

    4. Metabolic Labeling of Proteoglycans and Analysis of Their Synthesis and Sorting in Filter-Grown and Polarized Epithelial Cells

      • Kristian Prydz, Ravi Adusumalli
      Pages 25-36

    5. Fluorous-Tag-Assisted Synthesis of GAG-Like Oligosaccharides

      • José L. de Paz, Pedro M. Nieto
      Pages 37-47

    6. Aqueous Molecular Dynamics for Understanding Glycosaminoglycan Recognition by Proteins

      • Balaji Nagarajan, Umesh Desai
      Pages 49-62

    7. A Nonradioactive Method to Measure Hyaluronan Synthase Activity

      • Davide Vigetti, Evgenia Karousou, Manuela Viola, Alberto Passi
      Pages 63-70

    8. A Glycoproteomic Approach to Identify Novel Proteoglycans

      • Fredrik Noborn, Mahnaz Nikpour, Andrea Persson, Carina Sihlbom, Jonas Nilsson, Göran Larson
      Pages 71-85

    9. Analysis of Protein–Glycosaminoglycan Interactions Using Traveling Wave Ion-Mobility Mass Spectrometry

      • Robert V. Williams, I. Jonathan Amster
      Pages 87-92

    10. Ascidian (Chordata-Tunicata) Glycosaminoglycans: Extraction, Purification, Biochemical, and Spectroscopic Analysis

      • Felipe C. O. B. Teixeira, Mauro S. G. Pavão
      Pages 93-109

    11. Quantitative Disaccharide Profiling of Glycosaminoglycans from Two Different Preparations by PMP and Deuterated PMP Labeling

      • Meng Zhang, Yi An, Lijuan Zhang
      Pages 111-119

    12. Preparation and Characterization of Heparan Sulfate-Derived Oligosaccharides to Investigate Protein–GAG Interaction and HS Biosynthesis Enzyme Activity

      • Cédric Laguri, Rabia Sadir, Evelyne Gout, Romain R. Vivès, Hugues Lortat-Jacob
      Pages 121-137

    13. Heparan Sulfate Structure: Methods to Study N-Sulfation and NDST Action

      • Anders Dagälv, Anders Lundequist, Beata Filipek-Górniok, Tabea Dierker, Inger Eriksson, Lena Kjellén
      Pages 139-150

    14. Methods for the Production of Recombinant Heparosan, a Critical Heparin Precursor, from Nonpathogenic E. coli Strains

      • Anindita Roy, Varun Garg, Sai K. Mehta, Alessandro Rossi, Kuberan Balagurunathan
      Pages 151-161

    15. De Novo Sequencing of Heparin /Heparan Sulfate Oligosaccharides by Chemical Derivatization and LC-MS /MS

      • Quntao Liang, Joshua S. Sharp
      Pages 163-172

    16. Production and HPLC-Based Disaccharide Analysis of Xyloside-Primed Glycosaminoglycans

      • Andrea Persson, Emil Tykesson, Ulf Ellervik, Katrin Mani
      Pages 173-182

    17. Nuclear Magnetic Resonance Methods in Structural Characterization of Glycosaminoglycans

      • Vitor H. Pomin, Barbara Mulloy
      Pages 183-207

    18. Aggrecan: Approaches to Study Biophysical and Biomechanical Properties

      • Hadi Tavakoli Nia, Christine Ortiz, Alan Grodzinsky
      Pages 209-226

    19. Chromatographic Molecular Weight Measurements for Heparin , Its Fragments and Fractions, and Other Glycosaminoglycans

      • Barbara Mulloy, John Hogwood
      Pages 227-240
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Keywords

About this book

This second edition volume expands on the previous edition with new information on the structural analysis of glycosaminoglycans (GAGs); chemical and enzymatic synthesis of GAGs; biophysical, biochemical, and computational analysis of GAG-protein interactions; molecular and genetic approaches to manipulating GAG cell biology; and molecular and genetic methods involving animal models and organoids. This volume also offers insight and guidance to non-glycoscience researchers who are performing advanced experiments on GAGs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \

Cutting-edge and authoritative, Glycosaminoglycans: Methods and Protocols, Second Edition is a valuable experimental manual for practicing researchersincluding graduate and post-doctoral fellows, and provides them with the latest methodologies and practical tips to overcoming barriers in understanding the chemistry and biology of GAGs. 


Editors and Affiliations

  • Departments of Biology, Bioengineering, and Medicinal Chemistry, University of Utah, Salt Lake City, USA

    Kuberan Balagurunathan

  • Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, USA

    Hiroshi Nakato

  • Department of Medicinal Chemistry and Institute for Structural Biology, Drug Discovery and Development, Virginia Commonwealth University, Richmond, USA

    Umesh Desai

  • Department of Neurobiology & Department of Nutrition & Integrative Physiology, University of Utah, Salt Lake City, USA

    Yukio Saijoh

Bibliographic Information

  • Book Title: Glycosaminoglycans

  • Book Subtitle: Methods and Protocols

  • Editors: Kuberan Balagurunathan, Hiroshi Nakato, Umesh Desai, Yukio Saijoh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1398-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1397-9Published: 10 October 2021

  • Softcover ISBN: 978-1-0716-1400-6Published: 10 October 2022

  • eBook ISBN: 978-1-0716-1398-6Published: 09 October 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XXI, 829

  • Number of Illustrations: 66 b/w illustrations, 114 illustrations in colour

  • Topics: Biochemistry, general, Chemistry/Food Science, general

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