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A Differential Scanning Calorimetry (DSC) Experimental Protocol for Evaluating the Modified Thermotropic Behavior of Liposomes with Incorporated Guest Molecules

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Supramolecules in Drug Discovery and Drug Delivery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2207))

Abstract

Differential scanning calorimetry (DSC) is a well-established technique, suitable to monitor the interactions that may take place among the drug delivery systems of liposomes and the potential bioactive molecules that are incorporated inside them. Moreover, the DSC technique is considered to be a useful tool to characterize the thermal behavior of lipidic bilayers in the absence and presence of drugs and to highlight parameters, such as the cooperativity between the lipids and the guest molecules (i.e. drugs, polymers, dendrimers), providing also a prediction of the behavior of potential future drug delivery liposomal platforms. In this study, a protocol for DSC measurements on liposomal systems with incorporated guest molecules is described.

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Acknowledgments

This work has been co-financed by the European Union and Greek national funds through the program “Support for Researchers with Emphasis on Young Researchers” (call code: EDBM34, ΚΕ 14995) and under the research title “Preparation and study of innovative forms of administration of pharmaceutical molecules targeting at improved pharmacological properties.”

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Authors and Affiliations

  1. Section of Pharmaceutical Technology, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Zografou, Greece

    Maria Chountoulesi, Nikolaos Naziris & Costas Demetzos

  2. Department of Chemistry, Laboratory of Organic Chemistry, National and Kapodistrian University of Athens, Panepistimioupolis, Zografou, Greece

    Thomas Mavromoustakos

Authors
  1. Maria Chountoulesi
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  2. Nikolaos Naziris
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  3. Thomas Mavromoustakos
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  4. Costas Demetzos
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Corresponding author

Correspondence to Costas Demetzos .

Editor information

Editors and Affiliations

  1. Department of Chemistry, National and Kapodistrian University of Athens, Zografou, Greece

    Thomas Mavromoustakos

  2. Department of Chemistry Section of Organic Chemistry and Biochemistry, University of Ioannina, Ioannina, Greece

    Andreas G. Tzakos

  3. Computational Biology and Molecular Simulations Laboratory Department of Biophysics, School of Medicine, Bahcesehir University, Istanbul, Turkey

    Serdar Durdagi

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Chountoulesi, M., Naziris, N., Mavromoustakos, T., Demetzos, C. (2021). A Differential Scanning Calorimetry (DSC) Experimental Protocol for Evaluating the Modified Thermotropic Behavior of Liposomes with Incorporated Guest Molecules. In: Mavromoustakos, T., Tzakos, A.G., Durdagi, S. (eds) Supramolecules in Drug Discovery and Drug Delivery. Methods in Molecular Biology, vol 2207. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0920-0_21

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  • DOI: https://doi.org/10.1007/978-1-0716-0920-0_21

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0919-4

  • Online ISBN: 978-1-0716-0920-0

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