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Comparison of the thermal behavior and conformational changes in partially and fully hydrated dipalmitoylphosphatidylcholine systems

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Abstract

The temperature dependence of conformational changes for partially and fully hydrated DPPC systems through two physicochemical techniques, namely DSC and Raman spectroscopy, is studied. DSC experiments have shown a different thermal behavior between the two considered systems, indicating the effective role of water in the thermal behavior. A temperature resolution of inter- and intramolecular interactions during the main melting phase transition was achieved by using three different Raman intensity ratios, which confirm that the main phase transition represents a two-stage transition. Van’t Hoff plots for the C–C, C–H, C=O and C4N+ stretching modes, in a temperature range just below the main transition temperature, have been used to compare the thermodynamic parameters extracted by the two physicochemical techniques. The significance of these results can be summarized as follows: (a) DSC and Raman spectroscopy have shown complementary results indicating that DPPC exists in partially or fully hydrated states; (b) thermodynamic parameters ΔΗ and ΔS calculated in both techniques for the two different hydration states of DPPC were in harmony; (c) water more significantly affects the thermal and dynamic properties of fully hydrated DPPC bilayers than of the partially hydrated DPPC; and (d) water disturbs the head-group packing, the alkyl chains interactions and the mesophase region. It appears that the amount of water plays a vital role in the bilayer structure. As more and more extensive studies appear in the literature on biomolecules or drug membrane interactions, this information will be valuable in understanding the role of water in these interactions.

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Aknowledgements

This research was co-financed by the European Union (European Social Fund—ESF) and Greek National funds through the Operational Program “Education and Lifelong Learning” of the National Strategic Reference Framework (NSRF)—Research Funding Program: ARCHIMEDES III. Investing in knowledge society through the European Social Fund. The authors would like to acknowledge Ms. Shiri Arnon from UC Santa Barbara for proofreading the manuscript (Grant No. HTYSBIOP 021215).

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Authors and Affiliations

  1. Department of Chemistry, Laboratory of Physical Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis, 15771, Athens, Greece

    Petros Chatzigeorgiou & Kyriakos Viras

  2. Department of Food Technology, Technological Educational Institute of Ionian Islands, Vergotis Avenue, 28100, Argostoli, Kefalonia, Greece

    Athinaida Mourelatou & Evangelos Pollatos

  3. Department of Food Technology, Laboratory of Organic Chemistry, Technological Educational Institute of Athens, 12210, Egaleo, Greece

    Dimitra Margari & Charis E. Semidalas

  4. Department of Food Technology, Laboratory of Food Engineering, Technological Educational Institute of Athens, 12210, Egaleo, Greece

    Nikolaos Zogzas

  5. Department of Chemistry, Laboratory of Organic Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis, 15771, Athens, Greece

    Thomas Mavromoustakos

  6. Department of Chemistry, York College and the Graduate Center of the City, University of New York, 94-20 Guy R. Brewer Blvd., Jamaica, NY, 11451, USA

    Thomas Mavromoustakos

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  1. Petros Chatzigeorgiou
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Chatzigeorgiou, P., Mourelatou, A., Pollatos, E. et al. Comparison of the thermal behavior and conformational changes in partially and fully hydrated dipalmitoylphosphatidylcholine systems. J Therm Anal Calorim 131, 887–898 (2018). https://doi.org/10.1007/s10973-017-6622-8

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