Abstract
In this chapter, we detail the experimental protocol leading to the generation of stem cell-based mouse embryo-like structures termed “ETiX-embryoids.” ETiX-embryoids are formed from combined embryonic stem cells, trophoblast stem cells, and embryonic stem cells transiently induced to express Gata4. Cells are seeded into AggreWell dishes where they form aggregates that develop to resemble post-implantation mouse embryos following 4 days of culture. ETiX-embryoids establish an anterior signaling center and undergo gastrulation over the following 2 days. By day 7, ETiX-embryoids undergo neurulation and form an anterior-posterior axis with head folds at one end and a tail bud on the other. On day 8, they develop a brain and form a heart-like structure and a gut tube.
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Conflict of Interest
A patent application has been submitted by MZG, GA, and CH based on these results.
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Handford, C.E., Panda, P., Mohammad Choudhury, I.B., Amadei, G., Zernicka-Goetz, M. (2023). Generation of Stem Cell-Based Mouse Embryo-Like Structures. In: Zernicka-Goetz, M., Turksen, K. (eds) Embryo Models In Vitro. Methods in Molecular Biology, vol 2767. Humana, New York, NY. https://doi.org/10.1007/7651_2023_493
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DOI: https://doi.org/10.1007/7651_2023_493
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3685-5
Online ISBN: 978-1-0716-3686-2
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