Abstract
The development of single-cell multiomics has provided the ability to systematically investigate cellular diversity and heterogeneity in different biological systems via comprehensive delineations of individual cellular states. Single-cell RNA sequencing in particular has served as a powerful tool to the study of the molecular circuitries underlying preimplantation embryonic development in both the mouse and human. Here we describe a method to elucidate the cellular dynamics of the embryo further by performing both single-cell RNA sequencing (Smart-Seq2) and single-cell small non-coding RNA sequencing (Small-Seq) on the same individual embryonic cell.
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Acknowledgments
This work was supported by grants from the Canadian Institutes of Health Research (PJT-178082), the Swedish Research Council (2016-01919), and Swedish Society for Medical Research (Dnr4-236-2107). SP holds the Canada Research Chair in Functional Genomics of Reproduction and Development (950-233204) and KV was granted a NSERC PGS D scholarship.
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Table S1
Petropoulos sncRNA seq Indexes (XLSX 18 kb)
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Biondic, S. et al. (2023). Single-Cell mRNA-sncRNA Co-sequencing of Preimplantation Embryos. In: Zernicka-Goetz, M., Turksen, K. (eds) Embryo Models In Vitro. Methods in Molecular Biology, vol 2767. Humana, New York, NY. https://doi.org/10.1007/7651_2023_487
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DOI: https://doi.org/10.1007/7651_2023_487
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