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Generation of Human Blastoids from Naive Pluripotent Stem Cells

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Embryo Models In Vitro

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2767))

Abstract

Under certain culture conditions, naive human pluripotent stem cells can generate human blastocyst-like structures (called human blastoids). Human blastoids serve as an accessible model for human blastocysts and are amenable for large-scale production. Here, we describe a detailed step-by-step protocol for the robust and high-efficient generation of human blastoids from naive human pluripotent stem cells.

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References

  1. Gafni O et al (2013) Derivation of novel human ground state naive pluripotent stem cells. Nature 504:282–286. https://doi.org/10.1038/nature12745

    Article  CAS  PubMed  Google Scholar 

  2. Fu J, Warmflash A, Lutolf MP (2021) Stem-cell-based embryo models for fundamental research and translation. Nat Mater 20:132–144. https://doi.org/10.1038/s41563-020-00829-9

    Article  CAS  PubMed  Google Scholar 

  3. Yu L et al (2021) Blastocyst-like structures generated from human pluripotent stem cells. Nature 591:620–626. https://doi.org/10.1038/s41586-021-03356-y

    Article  CAS  PubMed  Google Scholar 

  4. Yu L, et al (2022) Large scale production of human blastoids amenable to modeling blastocyst development and maternal-fetal crosstalk. bioRxiv, 2022.2009.2014.507946. https://doi.org/10.1101/2022.09.14.507946

  5. Theunissen TW et al (2014) Systematic identification of culture conditions for induction and maintenance of naive human pluripotency. Cell Stem Cell 15:471–487

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Logsdon DM, Kile RA, Schoolcraft WB, Krisher RL, Yuan Y (2020) Single cell collection of trophoblast cells in peri-implantation stage human embryos. J Vis Exp. https://doi.org/10.3791/61476

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Author information

Authors and Affiliations

  1. Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Yulei Wei, Carlos A. Pinzon-Arteaga, Leqian Yu & Jun Wu

  2. State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China

    Yulei Wei

  3. Colorado Center for Reproductive Medicine, Lone Tree, CO, USA

    Kun Liu, Deirdre Logsdon & Ye Yuan

  4. Paul M. Rady Department of Mechanical Engineering, University of Colorado, Boulder, CO, USA

    Kun Liu

  5. State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China

    Leqian Yu

  6. Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China

    Leqian Yu

  7. Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Jun Wu

  8. Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Jun Wu

Authors
  1. Yulei Wei
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  2. Kun Liu
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  3. Carlos A. Pinzon-Arteaga
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  4. Deirdre Logsdon
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  5. Leqian Yu
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  6. Ye Yuan
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  7. Jun Wu
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Editor information

Editors and Affiliations

  1. Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA

    Magdalena Zernicka-Goetz

  2. Ottawa, ON, Canada

    Kursad Turksen

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Wei, Y. et al. (2023). Generation of Human Blastoids from Naive Pluripotent Stem Cells. In: Zernicka-Goetz, M., Turksen, K. (eds) Embryo Models In Vitro. Methods in Molecular Biology, vol 2767. Humana, New York, NY. https://doi.org/10.1007/7651_2023_485

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  • DOI: https://doi.org/10.1007/7651_2023_485

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3685-5

  • Online ISBN: 978-1-0716-3686-2

  • eBook Packages: Springer Protocols

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