Abstract
Induced pluripotent stem cells (iPSCs) are useful for the development of therapies in regenerative medicine, analysis of pathogenesis, and exploration of candidate drugs. We developed an alternative usage of iPSCs of which the MHC haplotype is matched to transplantable recipients in a cynomolgus macaque model. We established two cancer cell lines, embryonal carcinoma, and glioblastoma cell lines from cynomolgus monkey iPSCs. Here, we describe a method to induce the cancer cell lines including a technique for culture of the monkey iPSCs on feeder cells and the induction of hematopoietic stem cells and neural progenitor cells from monkey iPSCs.
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References
Navarrete EG, Liang P, Lan F, Sanchez-Freire V, Simmons C, Gong T et al (2013) Screening drug-induced arrhythmia using human induced pluripotent stem cell-derived cardiomyocytes and low-impedance microelectrode arrays. Circulation 128:S3–S13
Moretti A, Bellin M, Welling A, Jung CB, Lam JT, Bott-Flügel L et al (2010) Patient-specific induced pluripotent stem-cell models for long-QT syndrome. N Engl J Med 363:1397–1409
Paige SL, Thomas S, Stoick-Cooper CL, Wang H, Maves L, Sandstrom R et al (2012) A temporal chromatin signature in human embryonic stem cells identifies regulators of cardiac development. Cell 151:221–232
Ishigaki H, Maeda T, Inoue H, Akagi T, Sasamura T, Ishida H et al (2017) Transplantation of iPS-derived tumor cells with a homozygous MHC haplotype induces GRP94 antibody production in MHC-matched macaques. Cancer Res 77:6001–6010
Ishigaki H, Shiina T, Ogasawara K (2018) MHC-identical and transgenic cynomolgus macaques for preclinical studies. Inflamm Regen 38:30
Ebeling M, Küng E, See A, Broger C, Steiner G, Berrera M et al (2011) Genome-based analysis of the nonhuman primate Macaca fascicularis as a model for drug safety assessment. Genome Res 21:1746–1756
Yoshino N, Ami Y, Terao K, Tashiro F, Honda M (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp Anim 49:97–110
Uno Y, Iwasaki K, Yamazaki H, Nelson DR (2011) Macaque cytochromes P450: nomenclature, transcript, gene, genomic structure, and function. Drug Metab Rev 43:346–361
Donahue RE, Dunbar CE (2001) Update on the use of nonhuman primate models for preclinical testing of gene therapy approaches targeting hematopoietic cells. Hum Gene Ther 10:607–617
Casal M, Haskins M (2006) Large animal models and gene therapy. Eur J Hum Genet 14:266–272
Sasai K, Sukezane T, Yanagita E, Nakagawa H, Hotta A, Itoh T et al (2011) Oncogene-mediated human lung epithelial cell transformation produces adenocarcinoma phenotypes in vivo. Cancer Res 71:2541–2549
Okahara Narita J, Umeda R, Nakamura S, Mori T, Noce T, Torii R (2012) Induction of pluripotent stem cells from fetal and adult cynomolgus monkey fibroblasts using four human transcription factors. Primates 53:205–213
Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K et al (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861–872
Miller AD, Trauber DR, Buttimore C (1986) Factors involved in production of helper virus-free retrovirus vectors. Somat Cell Mol Genet 12:175–183
Akagi T (2004) Oncogenic transformation of human cells: shortcomings of rodent model systems. Trends Mol Med 10:542–548
Akagi T, Sasai K, Hanafusa H (2003) Refractory nature of normal human diploid fibroblasts with respect to oncogene-mediated transformation. Proc Natl Acad Sci U S A 100:13567–13572
Acknowledgments
This work was supported by Grants-in-Aid for Young Scientists (B) Grant number 23790442 and the Presidential Research Support 2010 and 2016 in Shiga University of Medical Science.
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Ishigaki, H., Itoh, Y. (2020). Embryonal Carcinoma and Glioblastoma Cell Lines Derived from Monkey Induced Pluripotent Stem Cells. In: Nagy, A., Turksen, K. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 2454. Humana, New York, NY. https://doi.org/10.1007/7651_2020_303
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DOI: https://doi.org/10.1007/7651_2020_303
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2118-9
Online ISBN: 978-1-0716-2119-6
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