Skip to main content
SpringerLink
Log in

Factors involved in production of helper virus-free retrovirus vectors

  • Published:
Somatic Cell and Molecular Genetics

Abstract

Retrovirus vectors allow efficient transfer of genetic material into cells. We describe an improved method for making cell lines which secrete broad host range retrovirus vectors in the absence of helper virus. This method was used to make virus-producing cell lines from several retrovirus vector constructions that encode dominant selectable markers. Virus titers from such lines exceeded 106 colony-forming units per milliliter of medium exposed to the cells. Cell lines that secreted certain vectors remained free of helper virus, while cell lines made using other vectors always secreted helper virus. Secretion of helper virus apparently depended on recombination between vector and the retrovirus packaging system, and factors involved in this event were investigated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature cited

  1. Joyner, A.L., and Bernstein, A. (1983).Mol. Cell. Biol. 3:2180–2190.

    PubMed  Google Scholar 

  2. Mann, R., Mulligan, R.C., and Baltimore, D. (1983).Cell 33:153–159.

    PubMed  Google Scholar 

  3. Miller, A.D., Jolly, D.J., Friedmann, T., and Verma, I.M. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:4709–4713.

    PubMed  Google Scholar 

  4. Miller, A.D., Law, M.F., and Verma, I.M. (1984).Mol. Cell. Biol. 5:431–437.

    Google Scholar 

  5. Shimotohno, K., and Temin, H.M. (1981).Cell 26:67–77.

    PubMed  Google Scholar 

  6. Tabin, C.J., Hoffmann, J.W., Goff, S.P., and Weinberg, R.I. (1982).Mol. Cell. Biol. 2:426–436.

    PubMed  Google Scholar 

  7. Wei, C.M., Gibson, M., Spear, P.G., and Scolnick, E.M. (1981).J. Virol. 39:935–944.

    PubMed  Google Scholar 

  8. Hellerman, J.G., Cone, R.C., Potts, J.T., Jr., Rich, A., Mulligan, R.C., and Kronenberg, H.M. (1984).Proc. Natl. Acad. Sci. U.S.A. 81:5340–5344.

    PubMed  Google Scholar 

  9. Miller, A.D., Ong, E.S., Rosenfeld, M.G., Verma, I.M., and Evans, R.M. (1984).Science 225:993–998.

    PubMed  Google Scholar 

  10. Weis, J.H., Reiss, C.S., Mulligan, R.C., Burakoff, S.J., and Seidman, J.G. (1985).Mol. Cell. Biol. 5:1379–1384.

    PubMed  Google Scholar 

  11. Episkopou, V., Murphy, A.J.M., and Efstratiadis, A. (1984).Proc. Natl. Acad. Sci. U.S.A. 81:4657–4661.

    PubMed  Google Scholar 

  12. Stuhlmann, H., Cone, R., Mulligan, R.C., and Jaenisch, R. (1984).Proc. Natl. Acad. Sci. U.S.A. 81:7151–7155.

    PubMed  Google Scholar 

  13. van der Putten, H., Botteri, F.M., Miller, A.D., Rosenfeld, M.G., Fan, H., Verma, I.M., and Evans, R.M. (1985).Proc. Natl. Acad. Sci. U.S.A. 82:6148–6152.

    PubMed  Google Scholar 

  14. Dick, J.E., Magli, M.C., Huszar, D., Phillips, R.A., and Bernstein, A. (1985).Cell 42:71–79.

    PubMed  Google Scholar 

  15. Keller, G., Paige, C., Gilboa, E., and Wagner, E.F. (1985).Nature 318:149–154.

    PubMed  Google Scholar 

  16. Miller, A.D., Eckner, R.J., Jolly, D.J., Friedmann, T., and Verma, I.M. (1984).Science 225:630–632.

    PubMed  Google Scholar 

  17. Williams, D.A., Lemischka, I.R., Nathan, D.G., and Mulligan, R.C. (1984).Nature 310:476–480.

    PubMed  Google Scholar 

  18. Cone, R.D., and Mulligan, R.C. (1984).Proc. Natl. Acad. Sci. U.S.A. 81:6349–6353.

    PubMed  Google Scholar 

  19. Mann, R., and Baltimore, D. (1985).J. Virol. 54:401–407.

    PubMed  Google Scholar 

  20. Sorge, J., Wright, D., Erdman, V.D., and Cutting, A.E. (1984).Mol. Cell. Biol. 4:1730–1737.

    PubMed  Google Scholar 

  21. Watanabe, S., and Temin, H.M. (1983).Mol. Cell. Biol. 3:2241–2249.

    PubMed  Google Scholar 

  22. Anderson, W.F. (1984).Science 226:401–409.

    PubMed  Google Scholar 

  23. Emerman, M., and Temin, H.M. (1984).J. Virol. 50:42–49.

    PubMed  Google Scholar 

  24. Emerman, M., and Temin, H.M. (1984).Cell 39:459–467.

    Google Scholar 

  25. Russell, W.C., Newman, C., and Williamson, D.H. (1975).Nature 253:461–462.

    PubMed  Google Scholar 

  26. Miller, A.D., and Verma, I.M. (1984).J. Virol. 49:214–222.

    PubMed  Google Scholar 

  27. Corsaro, C.M., and Pearson, M.L. (1981).Somat. Cell Genet. 7:603–616.

    PubMed  Google Scholar 

  28. Graham, F.L., and van der Eb, A.J. (1973).Virology 52:456–467.

    PubMed  Google Scholar 

  29. Hartley, J.W., and Rowe, W.P. (1976).J. Virol. 19:19–25.

    PubMed  Google Scholar 

  30. Colbère-Garapin, F., Horodniceanu, F., Kourilsky, P., and Garapin, A.-C. (1981).J. Mol. Biol. 150:1–14.

    PubMed  Google Scholar 

  31. Simonsen, C.C., and Levinson, A.D. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:2495–2499.

    PubMed  Google Scholar 

  32. Rasheed, S., Gardner, M.B., and Chan, E. (1976).J. Virol. 19:13–18.

    PubMed  Google Scholar 

  33. Hwang, L.S., and Gilboa, E. (1984).J. Virol. 50:417–424.

    PubMed  Google Scholar 

  34. Clark, S.P., and Mak, T.W. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:5037–5041.

    PubMed  Google Scholar 

  35. Shinnick, T.M., Lerner, R.A., and Sutcliffe, J.G. (1981).Nature 293:543–548.

    PubMed  Google Scholar 

  36. Rein, A., Schultz, A.M., Bader, J.P., and Bassin, R.H. (1982).Virology 119:185–192.

    PubMed  Google Scholar 

  37. Wolff, L., and Ruscetti, S. (1985).Science 228:1549–1552.

    PubMed  Google Scholar 

Download references

Author information

Author notes

  1. David R. Trauber

    Present address: Division of Hematology, Downstate Medical Center, State University of New York, 450 Clarkson Avenue, 11203, Brooklyn, New York

Authors and Affiliations

  1. The Fred Hutchinson Cancer Research Center, 1124 Columbia Street, 98104, Seattle, Washington

    A. Dusty Miller & Carol Buttimore

  2. Laboratory of Molecular Hematology, National Heart, Lung, and Blood Institute, 20892, Bethesda, Maryland

    David R. Trauber

Authors
  1. A. Dusty Miller
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. David R. Trauber
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Carol Buttimore
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Miller, A.D., Trauber, D.R. & Buttimore, C. Factors involved in production of helper virus-free retrovirus vectors. Somat Cell Mol Genet 12, 175–183 (1986). https://doi.org/10.1007/BF01560664

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01560664

Keywords

Search

Navigation