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Association of cotton leaf curl Multan betasatellite and Ageratum conyzoides symptomless alphasatellite with tomato leaf curl New Delhi virus in Luffa cylindrica in Pakistan

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Abstract

Luffa plants showing symptoms of begomovirus infection were collected from Vehari, Punjab in 2016. RCA and PCR amplifications of begomovirus genomic components showed the presence of a typical Old World bipartite begomovirus Tomato leaf curl New Delhi virus (ToLCNDV). Interestingly, Ageratum conyzoides symptomless alphasatellite and CLCuMB were also found associated with bipartite begomoviruses in the same host, however, no monopartite begomovirus was detected. To the best of our knowledge, this is the first report of ToLCNDV with associated satellites infecting L. cylindrica in Pakistan. The importance of begomovirus extended host range with different combinations of associated satellites has been discussed.

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Acknowledgments

This research was supported through research project No. 5584/Punjab/NRPU/R&D/HEC/2016 funded by Higher Education Commission (HEC) Pakistan, awarded to Khadim Hussain.

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Authors and Affiliations

  1. Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan

    Ifrah Anwar, Hanan Arif Bukhari, Imran Amin & Shahid Mansoor

  2. Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan

    Ifrah Anwar, Hanan Arif Bukhari, Nazia Nahid, Kamran Rashid & Khadim Hussain

  3. Department of Botany, Lahore College for Women University, Lahore, Pakistan

    Shabnum Shaheen

Authors
  1. Ifrah Anwar
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  2. Hanan Arif Bukhari
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  3. Nazia Nahid
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  4. Kamran Rashid
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  5. Imran Amin
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  6. Shabnum Shaheen
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  7. Khadim Hussain
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  8. Shahid Mansoor
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Contributions

Ifrah Anwar, Nazia Nahid, Imran Amin, Shabnum Shaheen, Khadim Hussain and Shahid Mansoor planned, designed and executed experimental work. Ifrah Anwar, Khadim Hussain conducted Data analysis. Ifrah Anwar, Khadim Hussain and Kamran Rashid wrote the manuscript.

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Correspondence to Khadim Hussain or Shahid Mansoor.

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Anwar, I., Bukhari, H.A., Nahid, N. et al. Association of cotton leaf curl Multan betasatellite and Ageratum conyzoides symptomless alphasatellite with tomato leaf curl New Delhi virus in Luffa cylindrica in Pakistan. Australasian Plant Pathol. 49, 25–29 (2020). https://doi.org/10.1007/s13313-019-00668-6

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  • DOI: https://doi.org/10.1007/s13313-019-00668-6

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