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Protective Effect of Intestinal Trefoil Factor on Injury of Intestinal Epithelial Tight Junction Induced by Platelet Activating Factor

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Abstract

Intestinal barrier dysfunction plays an important role in the pathogenesis of inflammatory bowel disease (IBD). To evaluate the effect of intestinal trefoil factor (ITF) on increased intestinal permeability and its association with tight junction proteins, an in vitro intestinal epithelia barrier model was established with Caco-2 cells and treated with platelet-activating factor (PAF). We found that exposing cells to 0.3 M ITF (30 min before or 30 min after PAF treatment) attenuated the PAF-induced changes in transepithelial electrical resistance and Lucifer yellow flux. A quantitative RT-PCR and western blot analysis revealed that ITF suppressed PAF-induced downregulation of tight junction proteins claudin-1 and ZO-1 expression; furthermore, an abnormal localization and distribution of these proteins was inhibited, as assessed by immunofluorescence staining. These results suggest that ITF decreases mucosal permeability and shows potential as a therapy for treating IBD.

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Acknowledgments

This work was supported by a research fund for the doctoral program from the National Ministry of Education (RFDP, 20092104110010), Educational Commission of Liaoning Province (L2010624), Doctorial Start Fund of Liaoning Province (20101147), and Science and Technology Planning Project of Shenyang (F10-205-1-45).

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  1. Department of Pediatric, Affiliated Shengjing Hospital, China Medical University, Shenyang, 110004, Liaoning, China

    Ling-fen Xu, Xu Teng, Jing Guo & Mei Sun

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  1. Ling-fen Xu
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  2. Xu Teng
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  4. Mei Sun
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Correspondence to Mei Sun.

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Xu, Lf., Teng, X., Guo, J. et al. Protective Effect of Intestinal Trefoil Factor on Injury of Intestinal Epithelial Tight Junction Induced by Platelet Activating Factor. Inflammation 35, 308–315 (2012). https://doi.org/10.1007/s10753-011-9320-x

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