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Diversity and pathogenicity of Colletotrichum species isolated from soursop in Colombia

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Abstract

Anthracnose, caused by Colletotrichum species is a highly limiting disease for the production of the tropical fruit tree crop, soursop (Annona muricata L.). In this study, 83 single-spore isolates of Colletotrichum were obtained from diseased soursoup tissues and subjected to a species complex-specific PCR assay. The isolates were identified as C. gloeosporioides sensu lato (n = 60), C. boninense s. lat. (n = 22), or C. acutatum s. lat. (n = 1). A subset of 21 selected isolates was identified to species level by means of a multi-locus phylogenetic analysis using sequences from the ITS region and partial sequences of the actin, β-tubulin-2, glyceraldehyde-3-phosphate dehydrogenase, and chitin synthase-1 genes. The multi-locus phylogenetic analysis resolved C. theobromicola, C. tropicale, C. siamense, and C. gloeosporioides sensu stricto in the C. gloeosporioides complex; C. karstii and one undetermined species in the C. boninense complex; as well as one undetermined species in the C. acutatum complex. Significant differences in anthracnose severity were observed between Colletotrichum species when tested for pathogenicity on attached twigs of soursop cv. Elita. Colletotrichum theobromicola and C. tropicale were associated with high and intermediate virulence, respectively, whereas the remaining species were associated with low virulence.

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Acknowledgments

We are grateful to Corporación BIOTEC, Ministerio de Agricultura y Desarrollo Rural de Colombia, Agencia Colombiana de Cooperación Internacional (ACCI), and COLCIENCIAS for their financial support. We thank Dr Jairo Castaño-Zapata for his contributions to this research; Juan B. Cuasquer (Systems Engineer, CIAT); and Elizabeth L. McAdam and Matthew Blair for reviewing the manuscript.

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Authors and Affiliations

  1. Plant Pathology, Tropical Fruit Project, International Center for Tropical Agriculture (CIAT), A.A. 6713, Cali, Colombia

    Elizabeth Álvarez, Lederson Gañán, Alberto Rojas-Triviño, Juan F. Mejía, Germán A. Llano & Alonso González

  2. Laboratorio de Fitopatología, Departamento de Producción Agropecuaria, Universidad de Caldas, A.A. 275, Manizales, Colombia

    Lederson Gañán

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  1. Elizabeth Álvarez
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Correspondence to Elizabeth Álvarez.

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Álvarez, E., Gañán, L., Rojas-Triviño, A. et al. Diversity and pathogenicity of Colletotrichum species isolated from soursop in Colombia. Eur J Plant Pathol 139, 325–338 (2014). https://doi.org/10.1007/s10658-014-0388-7

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