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Colletotrichum species associated with cultivated citrus in China

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Abstract

There have been considerable advances in the understanding of species concepts in the genus Colletotrichum. This has lead to the need to carry out fresh surveys of Colletotrichum species associated with important hosts. Colletotrichum species are associated with Citrus plants as saprobes, important pre-harvest and post-harvest pathogens, as well as endophytes. In this study, a total of 312 Colletotrichum strains were isolated from leaves, shoots and fruits of cultivated Citrus and Fortunella species with or without disease symptoms across the main citrus production areas in China. The morphology of all strains were studied and multilocus (ACT, TUB2, CAL, GAPDH, GS, ITS) phylogeny established. Strains were from four important species complexes of Colletotrichum, namely C. gloeosporioides species complex, C. boninense species complex, C. acutatum species complex and a final group including C. truncatum, which was rare on Citrus species. The species belonging to the C. gloeosporioides species complex comprised C. gloeosporioides and C. fructicola, the C. boninense complex comprised C. karstii and a new species C. citricola and the C. acutatum complex included a new species, C. citri. The ability of strains to cause anthracnose on citrus fruits was tested by inoculation and strains of Colletotrichum gloeosporioides, C. fructicola and C. truncatum were pathogenic.

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Acknowledgment

This work was supported by the China Agriculture Research System (CARS-27). L. Cai acknowledges grants KSCX2-YW-Z-1026 ⁄ NSFC31070020. Fang Liu is thanked for technical advices in the phylogenetic analyses. K.D. Hyde thanks the National Research Council of Thailand, Colletotrichum grant number 54201020003 for financial support.

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Authors and Affiliations

  1. Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China

    F. Huang, G. Q. Chen, X. Hou, Y. S. Fu & H. Y. Li

  2. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    L. Cai

  3. Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand

    K. D. Hyde

  4. School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand

    K. D. Hyde

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  1. F. Huang
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  2. G. Q. Chen
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Correspondence to H. Y. Li.

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Huang, F., Chen, G.Q., Hou, X. et al. Colletotrichum species associated with cultivated citrus in China. Fungal Diversity 61, 61–74 (2013). https://doi.org/10.1007/s13225-013-0232-y

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