Abstract
Distribution and taxonomic status of the Roosevelt’s Barking Deer (Muntiacus rooseveltorum) have remained poorly understood after more than 80 years since its description. All records of this species so far have been reported only from Lao PDR. During recent surveys in central Vietnam, we found several specimens from local hunting trophies morphologically resembling this species. Our molecular data, including both mitochondrial and nuclear genes, based on collected materials confirm for the first time that M. rooseveltorum is distributed in Vietnam. In addition, the phylogenetic analyses demonstrate that the Roosevelt’s Barking Deer represents a distinct evolutionary lineage closely related to the Truong Son Muntjac, in central Vietnam, and the Leaf Muntjac in Myanmar. Given the rarity of this species and the escalating hunting and habitat loss in the region, it is important to conduct field research to assess its population status. Such information is critically needed to design a conservation plan for this highly elusive and threatened taxon.
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References
Amato G, Egan MG, Rabinowitz A (1999a) A new species of muntjac, Muntiacus putaoensis (Artiodactyla: Cervidae) from northern Myanmar. Animal Conserv 2:1–7
Amato G, Egan MG, Schaller GB, Baker RH, Rosenbaum HC, Robichaud WG, Desalle R (1999b) Rediscovery of Roosevelt’s Barking Deer (Muntiacus rooseveltorum). J Mammal 80:639–643
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
Gatesy J (1997) More DNA support for a Cetacea/Hippopotamidae clade: the blood-clotting protein gene γ-fibrinogen. Mol Biol Evol 14:537–543
Giao PM, Tuoc D, Dung VV, Wikramanayake ED, Amato G, Arctander P, MacKinnon JR (1998) Description of Muntiacus truongsonensis, a new species of muntjac (Artiodactyla: Muntiacidae) from central Vietnam, and implications for conservation. Anim Conserv 1:61–68
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp 41:95–98
Hassanin A, Delsuc F, Ropiquet A, Hammer C, Jansen van Vuuren B, Matthee C, Ruiz-Garcia M, Catzeflis F, Areskoug V, Nguyen TT, Couloux A (2012) Pattern and timing of diversification of Cetartiodactyla (Mammalia, Laurasiatheria), as revealed by a comprehensive analysis of mitochondrial genomes. C R Biol 335:32–50
Hillis DM, Bull JJ (1993) An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Syst Biol 42:182–192
Huelsenbeck JP, Ronquist F (2001) MrBayes: Bayesian inference of phylogeny. Bioinformatics 17:754–755
Irwin DM, Kocher TD, Wilson AC (1991) Evolution of the cytochrome b gene of mammals. J Mol Evol 32:128–144
James J, Ramakrishnan U, Datta A (2008) Molecular evidence for the occurrence of the leaf deer Muntiacus putaoensis in Arunachal Pradesh, north-east India. Conserv Genet 9:927–931
Le TT, Le CV, Bui TD, Tran MH, Tran NQ, Nguyen SV, Monastyrskii AL, Eames JC (1999) A feasibility study for the establishment of Xuan Lien Nature Reserve, Thanh Hoa Province. Birdlife International Vietnam Programme, Hanoi
Le M, McCord WP, Iverson JB (2007) On the paraphyly of the genus Kachuga (Testudines: Geoemydidae). Mol Phylogenetics Evol 45:398–404
Osborn T, Fanning E, Grindley M (2000) Pu Hoat proposed nature reserve: biodiversity survey and conservation evaluation. Society for Environmental Education, London
Osgood W (1932) Mammals of the Kelley-Roosevelts and Delacour Asiatic expeditions. Field Museum of Natural History Publications 312. Zoological Series 18:192–339
Palumbi SR, Martin AP, Romano S, McMillan WO, Stice L, Grabowski G (1991) The simple fools guide to PCR, version 2.0. Privately published
Pang H, Liu W, Chen Y, Fang L, Zhang X, Cao X (2008) Identification of complete mitochondrial genome of the tufted deer. Mitochondrial DNA 19:411–417
Posada D, Crandall KA (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics 14:817–818
Randi E, Pierpaoli M, Danilkin A (1998) Mitochondrial DNA polymorphism in populations of Siberian and European roe deer (Capreolus pygargus and C. capreolus). Heredity 80:429–437
Swofford DL (2001) PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods), version 4. Sinauer Associates, Massachusetts
Timmins RJ, Duckworth JW, Long B (2008) Muntiacus rooseveltorum. In: IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. <www.iucnredlist.org>. Accessed 10 Sep 2013
Xiao CT, Zhang MH, Fu Y, Koh HS (2007) Mitochondrial DNA distinction of northeastern China roe deer, Siberian roe deer, and European roe deer, to clarify the taxonomic status of northeastern China roe deer. Biochem Genet 45:93–102
Acknowledgments
The Nagao Natural Environment Foundation, Japan, the SeaWorld and Busch Gardens Conservation Fund, Vietnam National University, and the Alfred P. Sloan Foundation generously provided funding for this project. Eleanor Sterling and Martha Hurley supported the early field work. M. Le was supported by the National Foundation for Science and Technology Development of Vietnam (NAFOSTED: Grant No. 106.15-2010.30). Comments from the associate editor and two anonymous reviewers helped improve the paper.
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Le, M., Nguyen, T.V., Duong, H.T. et al. Discovery of the Roosevelt’s Barking Deer (Muntiacus rooseveltorum) in Vietnam. Conserv Genet 15, 993–999 (2014). https://doi.org/10.1007/s10592-014-0581-4
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DOI: https://doi.org/10.1007/s10592-014-0581-4