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Determination of biogenic amines in Cheonggukjang using ultra high pressure liquid chromatography coupled with mass spectrometry

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Abstract

A rapid and reliable method for the simultaneous determination of 12 biogenic amines (BAs; agmatine, phenylethylamine, tyramine, putrescine, cadaverine, histamine, serotonin, tryptamine, spermidine, noreinephrine, dopamine, and spermine) in cheonggukjang was optimized and validated using ultra high pressure liquid chromatography-electrospray tandem mass spectrometry (UHPLC-ESI-MS/MS). The BAs were dansylated and separated on a C8 column under LC gradient of 7 min duration, and detected by MS/MS with the multiple reaction monitoring mode (MRM). This method exhibited excellent linearity for all of the analytes with correlation determination (R2) higher than 0.98. The limits of detection (LODs) were 10.8–39.6 μg/kg. The precision results were expressed as relative standard deviation (RSD), ranged from 0.3 to 14.3% for intra-day and from 0.9 to 15.4% for the interday. The proposed method will help to ensure for quality control by monitoring of BAs in cheonggukjang.

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References

  1. Silla Santos MH. Biogenic amines: Their importance in foods. Int. J. Food. Microbiol. 29: 213–231 (1996)

    Article  CAS  Google Scholar 

  2. Onal A. A review: Current analytical methods for the determination of biogenic amines in foods. Food. Chem. 103: 1475–1486 (2007)

    Article  Google Scholar 

  3. Romero R, Gázquez D, Bagur MG, Sánchez-Viñas M. Optimization of chromatographic parameters for the determination of biogenic amines in wines by reversed-phase high-performance liquid chromatography. J. Chromatogr. A 871: 75–83 (2000)

    Article  CAS  Google Scholar 

  4. Baek JG, Shim SM, Kwon DY, Choi HK, Lee CH, Kim YS. Metabolite profiling of cheonggukjang, a fermented soybean paste, inoculated with various Bacillus strains during fermentation. Biosci. Biotech. Bioch. 74: 1860–1868 (2010)

    Article  CAS  Google Scholar 

  5. Mayer HK, Fiechter G, Fischer E. A new ultra-pressure liquid chromatography method for the determination of biogenic amines in cheese. J. Chromatogr. A 1217: 3251–3257 (2010)

    Article  CAS  Google Scholar 

  6. Innocente N, Biasutti M, Padovese M, Moret S. Determination of biogenic amines in cheese using HPLC technique and direct derivatization of acid extract. Food. Chem. 101: 1285–1289 (2007)

    Article  CAS  Google Scholar 

  7. Cichy MA, Stegmeier DL, Veening H, Becker HD. High-performance liquid chromatographic separation of biogenic polyamines using 2-(1-pyrenyl)ethyl chloroformate as a new fluorogenic derivatizing reagent. J. Chromatogr. 613: 15–21 (1993)

    Article  Google Scholar 

  8. Hernández-Orte P, Peña-Gallego A, Ibarz MJ, Cacho J, Ferreira V. Determination of the biogenic amines in musts and wines before and after malolactic fermentation using 6-aminoquinolyl-Nhydroxysuccinimidyl carbamate as the derivatizing agent. J. Chromatogr. A 1129: 160–164 (2006)

    Article  Google Scholar 

  9. Pereira V, Pontes M, Câmara JS, Marques JC. Simultaneous analysis of free amino acids and biogenic amines in honey and wine samples using in loop orthophthalaldeyde derivatization procedure. J. Chromatogr. A 1189: 435–443 (2008)

    Article  CAS  Google Scholar 

  10. La Torre GL, Saitta M, Giorgia Potortì A, Di Bella G, Dugo G. High performance liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometry for sensitive determination of bioactive amines in donkey milk. J. Chromatogr. A 1217: 5215–5224 (2010)

    Article  Google Scholar 

  11. Latorre-Moratalla ML, Bosch-Fusté J, Lavizzari T, Bover-Cid S, Veciana-Nogués MT, Vidal-Carou MC. Validation of an ultra high pressure liquid chromatographic method for the determination of biologically active amines in food. J. Chromatogr. A 1216: 7715–7720 (2009)

    Article  CAS  Google Scholar 

  12. Cho TY, Han GH, Bahn KN, Son YW, Jang MR, Lee CH, Kim SH, Kim DB, Kim SB. Evaluation of biogenic amines in Korean commercial fermented foods. Korean. J. Food. Sci. Technol. 38: 730–737 (2006)

    Google Scholar 

  13. US DHHS. Center for Veterinary Medicine (CVM): Guidance for Industry, Bioanalytical Method. Center Evaluation and Research (CDER), Food and Drug Administration, US Department of Health and Human Service, Washington, DC, USA (2001)

    Google Scholar 

  14. ICH. Guidance for Industry, Q2B Validation of Analytical Procedures: Methodology. ICH-Q2B, Kuala Lumpur, Malaysia. pp. 71–76 (1996)

    Google Scholar 

  15. Guo K, Li L. Differential 12C-/13C-isotope dansylation labeling and fast liquid chromatography/mass spectrometry for absolute and relative quantification of the metabolome. Anal. Chem. 81: 3919–3932 (2009)

    Article  CAS  Google Scholar 

  16. Quirke JM, Adams CL, Van Berkel GV. Chemical derivatization for electrospray ionization mass spectrometry. 1. Alkyl halides, alcohols, phenols, thiols, and amines. Anal. Chem. 66: 1302–1315 (1994)

    Article  CAS  Google Scholar 

  17. Shukla S, Park HK, Kim JK, Kim M. Determination of biogenic amines in Korean traditional fermented soybean paste (doenjang). Food. Chem. Toxicol. 48: 1191–1195 (2010)

    Article  CAS  Google Scholar 

  18. Clausing P, Rushing LG, Newport GD, Bowyer JF. Determination of d-fenfluramine, d-norfenfluramine, and fluoxetuine in plasma, brain tissue, and brain microdialysate using high-performance liquid chromatography after precolumn derivatization with dansyl chloride. J. Chromatogr. B 592: 419–426 (1997)

    Google Scholar 

  19. Jones DP, Carlson JL, Samiec PS, Sternberg P Jr, Mody VC Jr, Reed RL, Brown LA. Glutathione measurement in human plasma Evaluation of sample collection, storage, and derivatization conditions for analysis of dansyl derivatives by HPLC. Clin. Chim. Acta 275: 175–184 (1998)

    Article  CAS  Google Scholar 

  20. Hernández-Borges J, D’Orazio G, Aturki Z, Fanali S. Nano-liquid chromatography analysis of dansylated biogenic amines in wines. J. Chromatogr. A 1147: 192–199 (2007)

    Article  Google Scholar 

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Authors and Affiliations

  1. Korea Food Research Institute, Seongnam, Gyeonggi, 463-746, Korea

    Sanghee Lee, Hye-Seon Eom, Miyoung Yoo, Yongsun Cho & Dongbin Shin

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  1. Sanghee Lee
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  2. Hye-Seon Eom
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  3. Miyoung Yoo
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  4. Yongsun Cho
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  5. Dongbin Shin
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Correspondence to Dongbin Shin.

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Lee, S., Eom, HS., Yoo, M. et al. Determination of biogenic amines in Cheonggukjang using ultra high pressure liquid chromatography coupled with mass spectrometry. Food Sci Biotechnol 20, 123–129 (2011). https://doi.org/10.1007/s10068-011-0017-6

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  • DOI: https://doi.org/10.1007/s10068-011-0017-6

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